The two divergent PEP-carboxylase catalytic subunits in the green microalga Chlamydomonas reinhardtii respond reversibly to inorganic-N supply and co-exist in the high-molecular-mass, hetero-oligomeric Class-2 PEPC complex

FEBS Letters

Volumn 581, Issue 25, 2007, Pages 4871-4876

  Moellering, Eric R ^a   Ouyang, Yexin ^a   Mamedov, Tarlan G ^a   Chollet, Raymond ^a  

^a UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

Anaplerosis; C N assimilation; Chlamydomonas reinhardtii; Green microalgae; PEP carboxylase

Indexed keywords

PHOSPHOENOLPYRUVATE CARBOXYLASE; POLYPEPTIDE; TETRAMER;

ARTICLE; CHLAMYDOMONAS REINHARDTII; CULTURE MEDIUM; DOWN REGULATION; ENZYME LOCALIZATION; ENZYME SUBUNIT; EUKARYOTE; GENE EXPRESSION; GENE IDENTIFICATION; INORGANIC NUTRIENT; MICROALGA; MOLECULAR WEIGHT; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; UPREGULATION;

ALGAL PROTEINS; ANIMALS; ANTIBODY SPECIFICITY; CATALYTIC DOMAIN; CHLAMYDOMONAS REINHARDTII; CULTURE MEDIA; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOBLOTTING; PHOSPHOENOLPYRUVATE CARBOXYLASE; PROTEIN DENATURATION; PROTEIN SUBUNITS; QUATERNARY AMMONIUM COMPOUNDS; ZEA MAYS;

CHLAMYDOMONAS REINHARDTII; EUKARYOTA;

EID: 34948821244     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2007.09.015     Document Type: Article

References (22)

1. Chollet R., Vidal J., and O'Leary M.H. Phosphoenolpyruvate carboxylase: a ubiquitous, highly regulated enzyme in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47 (1996) 273-298
2. Ettema T.J.G., Makarova K.S., Jellema G.L., Gierman H.J., Koonin E.V., Huynen M.A., de Vos W.M., and van der Oost J. Identification and functional verification of archaeal-type phosphoenolpyruvate carboxylase, a missing link in archaeal central carbohydrate metabolism. J. Bacteriol. 186 (2004) 7754-7762
3. Izui K., Matsumura H., Furumoto T., and Kai Y. Phosphoenolpyruvate carboxylase: a new era of structural biology. Annu. Rev. Plant. Biol. 55 (2004) 69-84
4. Guy R.D., Vanlerberghe G.C., and Turpin D.H. Significance of phosphoenolpyruvate carboxylase during ammonium assimilation. Carbon isotope discrimination in photosynthesis and respiration by the N-limited green alga Selenastrum minutum. Plant Physiol. 89 (1989) 1150-1157
5. Schuller K.A., Plaxton W.C., and Turpin D.H. Regulation of phosphoenolpyruvate carboxylase from the green alga Selenastrum minutum. Properties associated with replenishment of tricarboxylic acid cycle intermediates during ammonium assimilation. Plant Physiol. 93 (1990) 1303-1311
6. + assimilation rate and in vivo phosphoenolpyruvate carboxylase activity. Regulation of anaplerotic carbon flow in the green alga Selenastrum minutum. Plant Physiol. 94 (1990) 284-290
7. Huppe H.C., and Turpin D.H. Integration of carbon and nitrogen metabolism in plant and algal cells. Annu. Rev. Plant Physiol. Plant Mol. Biol. 45 (1994) 577-607
8. Norici A., Dalsass A., and Giordano M. Role of phosphoenolpyruvate carboxylase in anaplerosis in the green microalga Dunaliella salina cultured under different nitrogen regimes. Physiol. Plant 116 (2002) 186-191
9. Giordano M., Norici A., Forssen M., Eriksson M., and Raven J.A. An anaplerotic role for mitochondrial carbonic anhydrase in Chlamydomonas reinhardtii. Plant Physiol. 132 (2003) 2126-2134
10. Mamedov T.G., Moellering E.R., and Chollet R. Identification and expression analysis of two inorganic C- and N-responsive genes encoding novel and distinct molecular forms of eukaryotic phosphoenolpyruvate carboxylase in the green microalga Chlamydomonas reinhardtii. Plant J. 42 (2005) 832-843
11. Chen J.H., and Jones R.F. Multiple forms of phosphoenolpyruvate carboxylase from Chlamydomonas reinhardtii. Biochim. Biophys. Acta 214 (1970) 318-325
12. Rivoal J., Dunford R., Plaxton W.C., and Turpin D.H. Purification and properties of four phosphoenolpyruvate carboxylase isoforms from the green alga Selenastrum minutum: evidence that association of the 102-kDa catalytic subunit with unrelated polypeptides may modify the physical and kinetic properties of the enzyme. Arch. Biochem. Biophys. 332 (1996) 47-57
13. Rivoal J., Plaxton W.C., and Turpin D.H. Purification and characterization of high- and low-molecular-mass isoforms of phosphoenolpyruvate carboxylase from Chlamydomonas reinhardtii. Kinetic, structural and immunological evidence that the green algal enzyme is distinct from the prokaryotic and higher plant enzyme. Biochem. J. 331 (1998) 201-209
14. Rivoal J., Trzos S., Gage D.A., Plaxton W.C., and Turpin D.H. Two unrelated phosphoenolpyruvate carboxylase polypeptides physically interact in the high molecular mass isoforms of this enzyme in the unicellular green alga Selenastrum minutum. J. Biol. Chem. 276 (2001) 12588-12597
15. Blonde J.D., and Plaxton W.C. Structural and kinetic properties of high and low molecular mass phosphoenolpyruvate carboxylase isoforms from the endosperm of developing castor oilseeds. J. Biol. Chem. 278 (2003) 11867-11873
16. Tripodi K.E., Turner W.L., Gennidakis S., and Plaxton W.C. In vivo regulatory phosphorylation of novel phosphoenolpyruvate carboxylase isoforms in endosperm of developing castor oil seeds. Plant Physiol. 139 (2005) 969-978
17. Harris E.H. The Chlamydomonas Sourcebook: A Comprehensive Guide to Biology and Laboratory Use (1989), Academic Press, San Diego, California
18. Rivoal J., Smith C.R., Moraes T.F., Turpin D.H., and Plaxton W.C. A method for activity staining after native polyacrylamide gel electrophoresis using a coupled enzyme assay and fluorescence detection: application to the analysis of several glycolytic enzymes. Anal. Biochem. 300 (2002) 94-99
19. Schägger H., Cramer W.A., and von Jagow G. Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal. Biochem. 217 (1994) 220-230
20. Camacho-Carvajal M.M., Wollscheid B., Aebersold R., Steimle V., and Schamel W.W.A. Two-dimensional blue native/SDS gel electrophoresis of multi-protein complexes from whole cellular lysates. Mol. Cell. Proteomics 3 (2004) 176-182
21. McLellan T. Electrophoresis buffers for polyacrylamide gels at various pH. Anal. Biochem. 126 (1982) 94-99
22. Sánchez R., Flores A., and Cejudo F.J. Arabidopsis phosphoenolpyruvate carboxylase genes encode immunologically unrelated polypeptides and are differentially expressed in response to drought and salt stress. Planta 223 (2006) 901-909