A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii

Current Genetics

Volumn 53, Issue 6, 2008, Pages 381-388

  Shao, Ning ^a   Bock, Ralph ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

Author keywords

Bioluminescence; Chlamydomonas reinhardtii; Gaussia princeps; Heat inducible expression; Luciferase; Reporter gene

Indexed keywords

LUCIFERASE; NUCLEAR PROTEIN;

ARTICLE; BIOLUMINESCENCE; CHLAMYDOMONAS REINHARDTII; CODON; CODON USAGE; CONTROLLED STUDY; COPEPOD; ENVIRONMENTAL STRESS; FUNCTIONAL GENOMICS; GENE EXPRESSION; GENE EXPRESSION PROFILING; GENETIC ANALYSIS; GENETIC ENGINEERING; GENETIC TRANSFORMATION; GENETIC VARIABILITY; GREEN ALGA; HIGH THROUGHPUT SCREENING; IN VIVO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT GENETICS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN LOCALIZATION; PROTEIN PROTEIN INTERACTION; REPORTER GENE; SIGNAL TRANSDUCTION; TRANSGENE;

ANIMALS; CHLAMYDOMONAS REINHARDTII; CODON; COPEPODA; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENES, REPORTER; LUCIFERASES;

ALGAE; CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; CHLOROPHYTA; COPEPODA; GAUSSIA (COPEPOD); GAUSSIA PRINCEPS (T. SCOTT, 1894);

EID: 44349192711     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-008-0189-7     Document Type: Article

References (23)

1. Barnes D, Franklin S, Schultz J, Henry R, Brown E, Coragliotti A, Mayfield SP (2005) Contribution of 5′- and 3′-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes. Mol Genet Genomics 274:625-636
2. Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991-1995
3. Fischer N, Rochaix J-D (2001) The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii. Mol Genet Genomics 265:888-894
4. Fuhrmann M, Oertel W, Hegemann P (1999) A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii. Plant J 19:353-361
5. Fuhrmann M, Hausherr A, Ferbitz L, Schödl T, Heitzer M, Hegemann P (2004) Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene. Plant Mol Biol 55:869-881
6. Gutman BL, Niyogi KK (2004) Chlamydomonas and Arabidopsis. A dynamic duo. Plant Physiol 135:607-610
7. Harris EH (1989) The Chlamydomonas sourcebook. Academic Press, San Diego
8. Harris EH (2001) Chlamydomonas as a model organism. Annu Rev Plant Physiol Plant Mol Biol 52:363-406
9. Hippler M, Redding K, Rochaix J-D (1998) Chlamydomonas genetics, a tool for the study of bioenergetic pathways. Biochim Biophys Acta 1367:1-62
10. Kindle KL (1990) High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 87:1228-1232
11. Kropat J, Beck CF (1998) Characterization of photoreceptor and signaling pathway for light induction of the Chlamydomonas heat-shock gene HSP70A. Photochem Photobiol 68:414-419
12. Kropat J, Oster U, Rüdiger W, Beck CF (1997) Chlorophyll precursors are signals of chloroplast origin involved in light induction of nuclear heat-shock genes. Proc Natl Acad Sci USA 94:14168-14172
13. Mayfield SP, Schultz J (2004) Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast. Plant J 37:449-458
14. Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Maréchal-Drouard L, Marshall WF, Qu L-H, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Chlamydomonas Annotation Team, JGI Annotation Team, Grigoriev IV, Rokhsar DS, Grossman AR (2007) The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science 318:245-251
15. Minko I, Holloway SP, Nikaido S, Carter M, Odom OW, Johnson CH, Herrin DL (1999) Renilla luciferase as a vital reporter for chloroplast gene expression in Chlamydomonas. Mol Gen Genet 262:421-425
16. Porra RJ, Thompson WA, Kriedemann PE (1989) Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim Biophys Acta 975:384-394
17. Pröschold T, Harris EH, Coleman AW (2005) Portrait of a species: Chlamydomonas reinhardtii. Genetics 170:1601-1610
18. Remacle C, Cardol P, Coosemans N, Gaisne M, Bonnefoy N (2006) High-efficiency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes. Proc Natl Acad Sci USA 103:4771-4776
19. Remy I, Michnick SW (2006) A highly sensitive protein-protein interaction assay based on Gaussia luciferase. Nat Methods 3:977-979
20. Schroda M, Blöcker D, Beck CF (2000) The HSP70A promoter as a tool for the improved expression of transgenes in Chlamydomonas. Plant J 21:121-131
21. Shao N, Krieger-Liszkay A, Schroda M, Beck CF (2007) A reporter system for the individual detection of hydrogen peroxide and singlet oxygen: its use for the assay of reactive oxygen species produced in vivo. Plant J 50:475-487
22. Tannous BA, Kim D-E, Fernandez JL, Weissleder R, Breakefield XO (2005) Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol Therapy 11:435-443
23. von Gromoff ED, Treier U, Beck CF (1989) Three light-inducible heat shock genes of Chlamydomonas reinhardtii. Mol Cell Biol 9:3911-3918