Quenched ligand-directed tosylate reagents for one-step construction of turn-on fluorescent biosensors

Journal of the American Chemical Society

Volumn 131, Issue 25, 2009, Pages 9046-9054

  Tsukiji, Shinya ^a   Wang, Hangxiang ^a   Miyagawa, Masayoshi ^a   Tamura, Tomonori ^a   Takaoka, Yousuke ^a   Hamachi, Itaru ^a,b  

^a KYOTO UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY LIGANDS; ANALYTES; ANALYTICAL TOOL; BACTERIAL CELL LYSATE; BIOLOGICALLY RELEVANT MOLECULES; CARBONIC ANHYDRASE II; FLUORESCENCE QUENCHERS; LABELED PROTEINS; LABELING STEPS; NATURAL LIGANDS; NATURAL PROTEINS; NOVEL METHODS; ORGANIC DYE; PHOSPHOTYROSINE; PROTEIN FRAMEWORKS; PROTEIN LABELING; PROTEIN LIGANDS; SH2 DOMAIN; SPECIFIC DETECTION; SRC HOMOLOGY 2 DOMAINS; SYNTHETIC PROBES;

AMINES; FLUORESCENCE; LABELING; LIGANDS; PROTEINS; QUENCHING; SIGNAL TRANSDUCTION;

BIOSENSORS;

FLUORESCENT DYE; LIGAND; REAGENT; SULFONAMIDE; TOLUENESULFONIC ACID DERIVATIVE;

ARTICLE; BIOSENSOR; CHEMICAL STRUCTURE; CONJUGATION; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGAND BINDING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROTEIN ENGINEERING;

1-PHOSPHATIDYLINOSITOL 3-KINASE; AMINO ACID SEQUENCE; BIOSENSING TECHNIQUES; CARBONIC ANHYDRASE II; ENZYME INHIBITORS; ESCHERICHIA COLI; FLUORESCENT DYES; HUMANS; LIGANDS; MODELS, MOLECULAR; PHOSPHOPEPTIDES; PHOSPHOTYROSINE; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; TOSYL COMPOUNDS;

EID: 67649628228     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja902486c     Document Type: Article

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