Activation of endogenous Cdc42 visualized in living cells

Science

Volumn 305, Issue 5690, 2004, Pages 1615-1619

  Nalbant, Perihan ^a   Hodgson, Louis ^a   Kraynov, Vadim ^a   Toutchkine, Alexei ^a   Hahn, Klaus M ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSENSORS; CELLS; DYNAMICS;

SIGNALING PROTEINS; TRANS-GOLGI APPARATUS;

PROTEINS;

FLUORESCENT DYE; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE TRIPHOSPHATASE; PROTEIN CDC42; RAC PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; ACTIN; GREEN FLUORESCENT PROTEIN; MEROCYANINE DYE; PHOTOPROTEIN; PROTEIN; PYRIMIDINONE DERIVATIVE; RHO GUANINE NUCLEOTIDE BINDING PROTEIN; RHOQ PROTEIN, HUMAN; RHOQ PROTEIN, MOUSE; WAS PROTEIN, HUMAN; WAS PROTEIN, MOUSE; WISKOTT ALDRICH SYNDROME PROTEIN;

BIOLOGY;

ANIMAL CELL; ARTICLE; BIOSENSOR; CELL ADHESION; CELL FUNCTION; CELL SPREADING; CELL STRAIN 3T3; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DISSOCIATION CONSTANT; ENZYME ACTIVATION; ENZYME KINETICS; FLUORESCENCE SPECTROSCOPY; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN INTERACTION; TRANS GOLGI NETWORK; UMBILICAL VEIN; ALGORITHM; ANIMAL; CELL LINE; CELL MEMBRANE; CELL POLARITY; CELL SURFACE; CHEMISTRY; ENDOTHELIUM CELL; FIBROBLAST; FLUORESCENCE; GENETIC PROCEDURES; LEUKOCYTE ACTIVATION; METABOLISM; MICROTUBULE; NEUTROPHIL; PSEUDOPODIUM; SENSITIVITY AND SPECIFICITY; ULTRASTRUCTURE;

ANIMALIA;

ACTINS; ALGORITHMS; ANIMALS; BIOSENSING TECHNIQUES; CDC42 GTP-BINDING PROTEIN; CELL ADHESION; CELL LINE; CELL MEMBRANE; CELL POLARITY; CELL SURFACE EXTENSIONS; ENDOTHELIAL CELLS; FIBROBLASTS; FLUORESCENCE; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; LUMINESCENT PROTEINS; MICE; MICROTUBULES; NEUTROPHIL ACTIVATION; NEUTROPHILS; PROTEINS; PSEUDOPODIA; PYRIMIDINONES; RHO GTP-BINDING PROTEINS; SENSITIVITY AND SPECIFICITY; TRANS-GOLGI NETWORK; WISKOTT-ALDRICH SYNDROME PROTEIN;

EID: 4644266043     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1100367     Document Type: Article

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33. We are grateful to P. Hordijk, G. Bokoch, and L. Dehmelt for critical reading of the manuscript; to G. Bokoch for help with neutrophil stimulation assays; and to M. Berba for secretarial assistance. This work was funded by grants from the Deutsche Forschungsgemeinschaft (P.N.), the Arthritis Foundation (V.K.), the Leukemia and Lymphoma Society (A.T.), and by the NIH (grants GM57464 and GM64346) (K.M.H.). K.M.H. has a financial interest in and is a consultant for and member of the Scientific Advisory Board for Cenospectra Company, which seeks to develop products that precisely characterize molecular events that occur in cells.