Ratiometric fluorescent biosensor for real-time and label-free monitoring of fine saccharide metabolic pathways

ChemBioChem

Volumn 9, Issue 1, 2008, Pages 25-28

  Nakata, Eiji ^a   Wang, Hangxiang ^a   Hamachi, Itaru ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Biosensors; Fluorescent probes; Lectin; Ratiometric sensing; Saccharometabolism

Indexed keywords

CARBOHYDRATE; CARBOHYDRATE BINDING PROTEIN; CONCANAVALIN A; FLUORESCENT DYE; GLUCOKINASE; GLUCOSE; GLYCOLIPID; GLYCOPROTEIN; HYDROXYL GROUP; LECTIN; MANNOSE; MONOSACCHARIDE; NUCLEOTIDE; OLIGOSACCHARIDE; PHOSPHATE; PHOSPHORUS;

AGING; ARTICLE; BIOSENSOR; CARBOHYDRATE ANALYSIS; CARBOHYDRATE METABOLISM; CATALYSIS; FLUORESCENCE SPECTROSCOPY; GLYCOLYSIS; OXIDATIVE STRESS; PH MEASUREMENT; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; ULTRAVIOLET RADIATION;

ALKALINE PHOSPHATASE; BIOSENSING TECHNIQUES; CONCANAVALIN A; FLUORESCENT DYES; GLUCOSE; HYDROGEN-ION CONCENTRATION; KINETICS; LIGHT; MANNOSE; MASS SPECTROMETRY; METABOLIC NETWORKS AND PATHWAYS; MOLECULAR STRUCTURE; MONOSACCHARIDE TRANSPORT PROTEINS; MONOSACCHARIDES; PROTEIN STRUCTURE, QUATERNARY; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 38149121419     PISSN: 14394227     EISSN: 14397633     Source Type: Journal    
DOI: 10.1002/cbic.200700364     Document Type: Article

References (31)

1. a) C. R. Bertozzi, L. L. Kiessling, Science 2001, 291, 2357-2364;
2. b) R. A. Dwek, Chem. Rev. 1996, 96, 683-720;
3. c) D. B. Werz, P. H. Seeberger, Chem. Eur. J. 2005, 11, 3194-3206;
4. d) C. T. Campbell, K. J. Yarema, Genome Biol. 2005, 6, 236;
5. e) Y. Nagai, Glycoconjugate J. 2002, 19, 161-163.
6. a) C. Wu, S. A. Khan, A. J. Lange, Exp. Gerontol. 2005, 40, 894-899;
7. b) H. Kondoh, M. E. Lleonart, J. Gil, D. Beach, G. Peters, Drug Discovery Today Dis. Mech. 2005, 2, 263-267.
8. H. Schachter, J. Clin. Invest. 2001, 108, 1579 1582.
9. R. Jelinek, S. Kolusheva, Chem. Rev 2004, 104, 5987-6015.
10. S. Roseman, J. Biol. Chem. 2001, 276, 41527-41542.
11. a) R. P. Haugland in Handbook of Fluorescent Probes and Research Products, 9th ed., Molecular Probes, Eugene, 2002, pp. 833-836;
12. b) K. Honda, E. Nakata, A. Ojida, I. Hamachi, Chem. Commun. 2006, 4024-4026.
13. a) H. Lis, N. Sharon, Chem. Rev. 1998, 98, 637-674;
14. b) T. K. Dam, C. F. Brewer, Chem. Rev. 2002, 102, 387-429;
15. c) H. Rüdiger, H. J. Gabius, Glycoconjugate J. 2001, 18, 589-613.
16. a) I. Hamachi, T. Nagase, S, Shinkai, J. Am. Chem. Soc. 2000, 122, 12065-12066;
17. b) T. Nagase, S. Shinkai, I. Hamachi, Chem. Commun. 2001, 229-230;
18. c) T. Nagase, E. Nakata, S. Shinkai, I. Hamachi, Chem. Eur. J. 2003, 9, 3660-3669;
19. d) E. Nakata, T, Nagase, S, Shinkai, I. Hamachi. J. Am. Chem. Soc. 2004, 126, 490-495;
20. e) Y. Koshi, E. Nakata, I. Hamachi, ChemBioChem 2005, 6, 1349-1352;
21. f) E. Nakata, Y. Koshi, E, Koga, Y. Katayama, I. Hamachi, J. Am. Chem. Soc. 2005, 127, 13253-13261;
22. g) Y. Takaoka, H. Tsutsumi, N. Kasagi, E. Nakata, I. Hamachi, J. Am. Chem. Soc. 2006, 128, 3273-3280.
23. J. S. Rush, C. J. Waechter, Anal. Biochem. 1995, 224, 494-501.
24. [8b,f] cannot distinguish between the anomeric residues of sugars. In this point, SNARF-Con A is regarded to be a more sophisticated biosensor.
25. A. Nakata, M. Yamaguchi, K. Izutani, M, Amemura, J. Bacteriol. 1978, 134, 287-294.
26. [8f] was not able to monitor such reactions (See Figure S6 c). These control experiments clearly indicated the advantage of SNARF-Con A.
27. S. Karamohamed, G. Guidotti, BioTechniques 2001, 31, 420-425.
28. H. Hengartner, H. Zuber, FEBS Lett. 1973, 37, 212-216.
29. G. G. Hardy, Infect. Immun. 2001, 69, 2309-2317.
30. a) C. R. Goward, R. Hartwell, T. Atkinson, M. D. Scawen, Biochem. J. 1986, 237, 415-420;
31. b) O. H. Lowry, J. V. Passonneau, J. Biol. Chem. 1969, 244, 910-916.