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Volumn 6, Issue 8, 2005, Pages 1349-1352

Luminescent saccharide biosensor by using lanthanide-bound lectin labeled with fluorescein

Author keywords

Biosensors; Lanthanides; Lectins; Luminescence; Protein engineering

Indexed keywords

CARBOHYDRATE; CARBOHYDRATE BINDING PROTEIN; FLUORESCEIN; FLUORESCENT DYE; GLYCOCONJUGATE; LANTHANIDE; LECTIN;

EID: 23644434252     PISSN: 14394227     EISSN: None     Source Type: Journal    
DOI: 10.1002/cbic.200500072     Document Type: Article
Times cited : (28)

References (32)
  • 6
    • 4544326532 scopus 로고    scopus 로고
    • Angew. Chem. Int. Ed. 2004, 43, 3682-3685;
    • (2004) Angew. Chem. Int. Ed. , vol.43 , pp. 3682-3685
  • 19
    • 33645170011 scopus 로고    scopus 로고
    • note
    • The fluorescence quantum yield (QY) of fluorescein is not greatly lessened by attachment to a Con A surface, that is, QY of fluorescein - 0.95, QY of fluorescein-5-maleimide - 0.47, QY of FI-Con A 0.52 (see Figure S2 in the Supporting Information).
  • 24
    • 33645184802 scopus 로고    scopus 로고
    • note
    • 7-Dimetylaminocomarin-4-acetic acid (see Figure S5a in the Supporting Information).
  • 25
    • 33645169913 scopus 로고    scopus 로고
    • note
    • As a standard method, the wavelength at 490 nm (energy donor) was used for quantitative analysis. However, the emission peak at 490 nm overlaps partially with the FI emission to make the precise analysis difficult. Thus, we normalized the spectra using the peak at 544 nm instead of 490 nm in this study.
  • 26
    • 33645171145 scopus 로고    scopus 로고
    • note
    • It is known that UV excitation is sometimes problematic because of the UV-induced strong photobleaching. However, we confirmed that the photobleaching of the emission of FI-Con A by 280 nm excitation scarcely occurred under our measurement conditions (see Figure S6 in the Supporting Information). Thus, it is considered that the luminescent ratio change in the titration experiments was not affected by this factor.
  • 27
    • 33645175852 scopus 로고    scopus 로고
    • note
    • III in these titrations, because the luminescence spectra of native Con A/Tb were never changed by the addition of Man-1 (see Figure S8 in the Supporting Information).
  • 28
    • 33645176300 scopus 로고    scopus 로고
    • note
    • When Fl-Con A was titrated against Man type saccharides, the fluorescence intensity was gradually lessened and saturated (e.g. Man-5, see Figure 59 in the Supporting Information). This spectral change is the same as that of IAEDANS-Con A, and thus the mechanism for the fluorescence change, that is, the fluorophore being pushed out by the sugar, as with IAEDANS-Con A, is suggested in this case.
  • 30
    • 0014027154 scopus 로고
    • a) Y. T. Li, J. Biol. Chem. 1966, 247, 1010-1012;
    • (1966) J. Biol. Chem. , vol.247 , pp. 1010-1012
    • Li, Y.T.1
  • 32
    • 33645173765 scopus 로고    scopus 로고
    • note
    • The reaction was also monitored by MALDI-TOF MS. The time course was in agreement with that monitored by LRET.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.