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Volumn 126, Issue 2, 2004, Pages 490-495

Coupling a Natural Receptor Protein with an Artificial Receptor to Afford a Semisynthetic Fluorescent Biosensor

Author keywords

[No Author keywords available]

Indexed keywords

BIOSENSORS; FLUORESCENCE; PROTEINS;

EID: 0346456883     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja035631i     Document Type: Article
Times cited : (65)

References (25)
  • 14
    • 0043032818 scopus 로고    scopus 로고
    • (c) The conventional peptide digestion, HPLC separation, and tandem mass spectroscopy indicate that the photolabeling site can be assigned to Y100, a proximal amino acid in the saccharide-binding pocket. It is clear that the photoaffinity labeling process of the present P-PALM method is remarkably site-selective on a protein surface. Nagase, T.; Nakata, E.; Shinkai, S.; Hamachi, I. Chem.-Eur. J. 2003, 9, 3660.
    • (2003) Chem.-Eur. J. , vol.9 , pp. 3660
    • Nagase, T.1    Nakata, E.2    Shinkai, S.3    Hamachi, I.4
  • 15
    • 11544358874 scopus 로고    scopus 로고
    • The crystal data of ConA complexed with Man-tri showed the extended interaction between Man-tri and the ConA surface using all the OH groups of Man-tri and several amino acid residues of the ConA surface, The lessened flexibility of the branched Man units of Man-tri due to such an extended interaction may be a factor for no sensing to Man-tri. Lis, H.; Sharon, N. Chem. Rev. 1998, 98, 637.
    • (1998) Chem. Rev. , vol.98 , pp. 637
    • Lis, H.1    Sharon, N.2
  • 25
    • 0346464981 scopus 로고    scopus 로고
    • note
    • Since the fluorescence signal from APET-ConA is produced by a saccharide-binding event at the PBA site, not at the native ConA pocket (Figure 1a), the binding constants are evaluated on the basis of the saccharide binding at the PBA-modified ConA site. Although the other unmodified binding site (i.e., without PBA) may bind saccharides with a weak affinity, it does not affect the determination of binding constants under conditions of the excess amount of saccharide (for Benesi-Hildebrandt analysis). In case of the strong binding affinity observed at the almost stoichiometric amount of the saccharide, the affinity may be slightly underestimated because the native binding pocket binds the corresponding saccharide without fluorescence sensing so as to decrease the free concentration of the saccharide.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.