Molecular diagnosis of transgenic tomato with osmotin gene using multiplex polymerase chain reaction

Current Science

Volumn 96, Issue 5, 2009, Pages 689-694

  Randhawa, Gurinder Jit ^a   Singh, Monika ^a   Chhabra, Rashmi ^a   Guleria, Smriti ^a   Sharma, Ruchi ^a  

^a NATIONAL BUREAU OF PLANT GENETIC RESOURCES   (India)

Author keywords

Genetically modified tomato; Molecular diagnosis; Multiplex PCR; Osmotin gene

Indexed keywords

CAULIFLOWER MOSAIC VIRUS; LYCOPERSICON ESCULENTUM; NICOTIANA TABACUM;

EID: 67649240600     PISSN: 00113891     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (33)

1. James, C., Global status of commercialised biotech/GM crops 2008. ISAAA Brief No. 39, ISAAA, Ithaca, NY, 2008.
2. Frewer, L., Lassen, J., Kettlitz, B., Scholderer, J., Beekman, V. and Berdal, K. G., Social aspects of genetically modified foods. Food Chem. Toxicol., 2004, 42, 1181-1193.
3. Tao, Z., Cai, X. F., Yang, S. L. and Gong, Y., Detection of exogenous genes in genetically modified plants with multiplex polymerase chain reaction. Plant Mol. Biol. Rep., 2001, 19, 289-298.
4. Matsuoka, T. et al., A method of detecting recombinant DNAs from four lines of genetically modified maize. J. Food Hyg. Soc. Jpn., 2000, 41, 137-143.
5. Hubner, P., Waiblinger, H. U., Pietsch, K. and Brodmann, P., Validation of PCR methods for quantitation of genetically modified plants in food. J. AOAC Int., 2001, 84, 1855-1864.
6. Akiyama, H., Sugimoto, K., Matsumoto, M., Isuzugawa, K., Shibuya, M., Goda, Y. and Toyoda, M., A detection method of recombinant DNA from genetically modified potato (NewLeaf Y-Potato). J. Food Hyg. Soc. Jpn., 2002, 44, 24-29.
7. Matsuoka, T. et al., Detection of recombinant DNA segments introduced to genetically modified maize (Zea mays). J. Agric. Food Chem., 2002, 50, 2100-2109.
8. Permingeat, H. R., Reggiardo, M. I. and Vallejos, R. H., Detection and quantification of transgenes in grains by multiplex and real time PCR. J. Agric. Food Chem., 2002, 50, 4431-4436.
9. Watanabe, T., Kasama, K., Wakui, C., Shibuya, M., Matsuki, A., Akiyama, H. and Maitani, T., Laboratory-performance study of the notified methods to detect genetically modified maize (CBH351) and potato (NewLeaf Plus and NewLeaf Y). Shokuhin Eiseigaku Zasshi (in Japanese), 2003, 44, 281-288.
10. Yoshimura, T. et al., Applicability of the quantification of genetically modified organisms to foods processed from maize and soy. J. Agric. Food Chem., 2005, 53, 2052-2059.
11. Randhawa, G. J. and Firke, P. K., Detection of transgenes in genetically modified soybean and maize using polymerase chain reaction. Indian J. Biotechnol., 2006, 5, 510-513.
12. Sarad, N., Rathore, M., Singh, N. K. and Kumar, N., Genetically engineered tomatoes: New vista for sustainable agriculture in high altitude regions. In New Directions for a Diverse Planet: Proceedings of the 4th International Crop Science Congress, Brisbane, Australia, 26 September - 1 October 2004.
13. Tayal, D., Srivastava, P. S. and Bansal, K. C., Transgenic crops for abiotic stress tolerance. In Plant Biotechnology and Molecular Markers, Springer, Netherlands, 2006, pp. 346-365.
14. Development of transgenic crops for biotic and abiotic stress resistance. ICAR News, 2006, 12, 9.
15. Bressan, R. A., Singh, N. K., Handa, A. K., Mount, R., Clithero, J. and Hasegawa, P. M., Stability of altered gene expression in cultured plant cells adapted to salt. In Drought Resistance in Plants, Physiological and Genetic Aspects (eds Monti, L. and Porceddu, E.), Commission of the European Communities, Brussels, 1987, pp. 41-57.
16. Barthakur, S., Babu, V. and Bansal, K. C., Over-expression of osmotin induces proline accumulation and confers tolerance to osmotic stress in transgenic tobacco. J. Plant Biochem. Biotechnol., 2001, 10, 31-37.
17. Singh, N. K. et al., Characterization of osmotin: a thaumatin-like protein associated with osmotic adaptation in plant cells. Plant Physiol., 1987, 85, 529-536.
18. Ouyang, B., Chen, Y. H., Li, H. X., Qian, C. J., Huang, S. L. and Ye, Z. B., Transformation of tomatoes with osmotin and chitinase genes and their resistance to Fusarium wilt. J. Hortic. Sci. Biotechnol., 2005, 80, 517-522.
19. Bonfini, L., Petra, H., Kay, S. and Van den Eede, G., Review of GMO detection and quantification techniques. EUR 20384 EN, 2002.
20. Twell, D., Yamaguchi, J. and McCormick, S., Pollen-specific gene expression in transgenic plants: coordinate regulation of two different tomato gene promoters during microsporogenesis. Development, 1990, 109, 705-713.
21. Yang, L. et al., Validation of a tomato-specific gene, LAT52, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic tomatoes. J. Agric. Food Chem., 2005, 53, 183-190.
22. Chamberlain, J. S., Gibbs, R. A., Ranier, J. E., Nguyen, P. N. and Caskey, C. T., Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res., 1988, 16, 11141-11156.
23. Burns, M., Shanahan, D., Valdivia, H. and Harris N., Quantitative event-specific multiplex PCR detection of Roundup Ready soya using LabChip technology. Eur. Food Res. Technol., 2003, 216, 428-433.
24. Dainese, E., Angelucci, C., de Santis, P., Maccarrone, M. and Cozzani, I., A multiplex PCR-based assay for the detection of genetically modified soybean. Anal. Lett., 2004, 37, 1139-1150.
25. Germini, A., Zanetti, A., Salati, C., Rossi, S., Forré, C., Schmid, S. and Marchelli, R., Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods. J. Agric. Food Chem., 2004, 52, 3275-3280.
26. Forte, V. T., Pinto, A. D., Martino, C., Tantillo, G. M., Grasso, G. and Schena, F. P., A general multiplex-PCR assay for the general detection of genetically modified soya and maize. Food Control, 2005, 16, 535-539.
27. Delano, J., Anna-Mary, S., Erika, W., Margaret, G. and Saad, M., Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis. J. Agric. Food Chem., 2003, 51, 5829-5834.
28. Wall, E., Lawrence, T., Green, M. and Rott, M., Detection and identification of transgenic virus resistant papaya and squash by multiplex PCR. Eur. Food Res. Technol., 2004, 219, 90-96.
29. Yang, L. et al., Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531. Transgenic Res., 2005, 14, 817-831.
30. Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A. and Allard, R. W., Ribosomal DNA spacer length polymorphism in barley, Mendelian inheritance, chromosomal location and population dynamics. Proc. Natl. Acad. Sci. USA, 1984, 81, 8014-8019.
31. Lipp, M., Brodmann, P., Pietsch, K., Pauwels, J. and Anklam, E., IUPAC collaborative trial study of a method to detect genetically modified soybeans and maize in dried powder. J. AOAC Int., 1999, 82, 923-928.
32. Matsuoka, T. et al., A multiplex PCR method of detecting recombinant DNAs from five lines of genetically modified maize. J. Food Hyg. Soc. Jpn., 2001, 42, 24-32.
33. Yang, L., Shen, H., Pan, A., Chen, J., Huang, C. and Zhang, D., Screening and construct-specific detection methods of transgenic Huafan No 1 tomato by conventional and real-time PCR. J. Sci. Food Agric., 2005, 85, 2159-2166.