Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531

Transgenic Research

Volumn 14, Issue 6, 2005, Pages 817-831

  Yang, Litao ^a,b   Pan, Aihu ^c   Zhang, Kewei ^c   Yin, Changsong ^c   Qian, Bingjun ^a   Chen, Jianxiu ^b   Huang, Cheng ^b   Zhang, Dabing ^a,c  

^a SHANGHAI JIAO TONG UNIVERSITY   (China)

^b NANJING UNIVERSITY   (China)

^c INSTITUTE OF EDIBLE FUNGI   (China)

Author keywords

Genetically modified cotton; Mon1445; Mon531; Multiplex PCR; Reference molecule; TaqMan real time PCR

Indexed keywords

HERBICIDE; PLANT DNA; VEGETABLE PROTEIN;

ANIMAL; ARTICLE; BIOLOGICAL PEST CONTROL; CHEMISTRY; COMPARATIVE STUDY; COTTON; DRUG RESISTANCE; GENETICS; INSECT; INSECTICIDE RESISTANCE; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PLANT SEED; POLYMERASE CHAIN REACTION; STANDARD; TRANSGENIC PLANT;

ANIMALS; BASE SEQUENCE; DNA, PLANT; DRUG RESISTANCE; GOSSYPIUM; HERBICIDES; INSECTICIDE RESISTANCE; INSECTS; MOLECULAR SEQUENCE DATA; PEST CONTROL, BIOLOGICAL; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; SEEDS;

GOSSYPIUM HIRSUTUM; INSECTA;

EID: 28244481915     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-005-0010-z     Document Type: Article

References (41)

1. E Anklam F Gadani P Heinze H Pijnenburg G Van den Eede 2002 Analytical methods for detection and determination of genetically modified organism in agricultural crops and plant-derived food products Eur Food Res Technol 214 3 26
2. K Arumuganathan ED Earle 1991 Nuclear DNA content of some important plant species Plant Mol Biol Rep 9 208 218
3. Beazleyn KA, Hillyard JR, Pang S and Roberts JK (2004) Cotton event PV-GHBK04 (757) and compositions and methods for detection thereof. US Patent. Pub. No.: US2004/0045054 A1
4. ® soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses Eur Food Res Technol 213 432 438
5. J Ding J Jia L Yang H Wen C Zhang W Liu D Zhang 2004 Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes J Agric Food Chem 52 3372 3377
6. European Commission, 2003a Commission Regulation (EC) No. 1829/2003 of September 22, 2003, Concerning on genetically modified food and feed Off. J. Eur. Commun L268 1 23
7. European Commission, 2003b Commission Regulation (EC) No. 1830/2003 of September 22, 2003, Concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC Off. J. Eur. Commun L268 24 28
8. G Gaskell MW Bauer J Durant NC Allum 1999 Worlds apart? The reception of genetically modified foods in Europe and the U. S Science 285 384 386
9. JL Harwood 1988 Fatty acid metabolism Ann Rev Plant Physiol Plant Mol Biol 39 101 138
10. M Hernández A Río T Esteve S Prat M Pla 2001 A rapeseed-specific gene, Acetyl-CoA Carboxylase, can be used as a reference for qualitative and real-Time quantitative PCR detection of transgenes from mixed food samples J Agric Food Chem 49 3622 3627
11. ® based on the 3′-transgene integration sequence Transgenic Res 12 179 189
12. A Holck M Vaitilingom L Didierjean K Rudi 2002 5′-Nuclease PCR for quantitative event-specific detection of the genetically modified Mon810 MaisGard maize Eur Food Res Technol 214 449 454
13. HY Huang TM Pan 2004 Detection of genetically modified maize MON810 and NK603 by multiplex and real-time polymerase chain reaction methods J Agric Food Chem 52 3264 3268
14. J Huang S Rozelle C Pray Q Wang 2002 Plant biotechnology in China Science 295 674 677
15. E Ido M Hayami 1997 Construction of T-tailed vectors derived from a pUC plasmid: a rapid system for direct cloning of unmodified PCR products Biosci Biotechnol Biochem 61 1766 1767
16. James C (2005) Global status of commercialized Biotech/GM crops. ISAAA Briefs No. 32
17. D James AM Schmidt E Wall M Green S Masri 2003 Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis J Agric Food Chem 51 5829 5834
18. A Jankiewicz H Broll J Zagon 1999 The official method for the detection of genetically modified soybeans (German Food Act LMBG § 35): a semi-quantitative study of sensitivity limits with glyphosate-tolerant soybeans (Roundup Ready) and insect-resistant Bt maize (Maximizer) Eur Food Res Technol 209 77 82
19. H Kuribara Y Shindo T Matsuoka K Takubo S Futo N Aoki T Hirao H Akiyama Y Goda M Toyoda A Hino 2002 Novel reference molecules for quantitation of genetically modified maize and soybean J AOAC Int 85 1077 1189
20. Q Liu SP Singh PJ Sharp AG Green DR Marshall 1996 Nucleotide sequence of a cDNA from Gossypium hirsutum encoding a stearoyl-acyl carrier protein desaturase Plant Physiol 110 1436
21. Matsuoka T (2001) GMO labeling and detection methods in Japan. APEC-JIRCAS Joint Symposium and Workshop on Agricultural Biotechnology
22. Ministry of Agriculture and Forestry (2000) Guidelines for Labeling of Genetically Modified Agricultural Products. MAF Notification No. 31
23. Monsanto Company (2002a) Safety Assessment of Roundup Ready® Cotton Event 1445
24. Monsanto Company (2002b) Safety Assessment of Bollgard® Cotton Event 531
25. A Pan D Zhang L Pan J Chen Z Yuan W Liang 2003 A rapid PCR method for detection of Glyphosate resistant transgenic rape Agric Sci China 36 856 860
26. D Proudnikov V Yuferov Y Zhou KS LaForge A Ho MJ Kreek 2003 Optimizing primer-probe design for fluorescent PCR J Neurosci Methods 123 31 45
27. Rangwala TS and Ye M (2002) Cotton event PV-GHGT07 (1445) and compositions and methods for detection thereof. International Patent Pub. No.: WO 02/34946 A2
28. JK Rho T Lee SI Jung TS Kim YH Park YM Kim 2004 Qualitative and quantitative PCR methods for detection of three lines of genetically modified potatoes J Agric Food Chem 52 3269 3274
29. SB Rønning KG Berdal A Holst-Jensen 2003 Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays) Eur Food Res Technol 216 347 354
30. DS Smith PW Maxwell SH De Boer 2004 Method for the detection of synthetic cry3A in transgenic potatoes J Agric Food Chem 52 809 815
31. I Taverniers P Windels E Van Bockstaele M De Loose 2001 Use of cloned DNA fragments for event-specific quantification of genetically modified organisms in pure and mixed food products Eur Food Res Technol 213 417 424
32. C Terry N Harris 2001 Event-specific detection of Roundup Ready Soya using two different real time PCR detection chemistries Eur Food Res Technol 213 425 431
33. H Weng A Pan L Yang C Zhang Z Liu D Zhang 2004 Estimating number of transgene copies in transgenic rapeseed by real-time PCR assay With HMG I/Y as an endogenous reference gene Plant Mol Biol Rep 22 289 300
34. H Weng L Yang Z Liu J Ding A Pan D Zhang 2005 A novel reference gene, highmobility-group protein I/Y, can be used in qualitative and real-time quantitative PCR detection of transgenic rapeseed cultivars J AOAC Int 88 577 584
35. P Windels S Bertrand A Depicker W Moens E Van Bockstaele M De Loose 2003 Qualitative and event-specific PCR real-time detection methods for StarLink™ maize Eur Food Res Technol 216 259 263
36. C Wolf M Scherzinger A Wurz U Pauli P Hubner J Luthy 2000 Detection of cauliflower mosaic virus by the polymerase chain reaction: testing of food components for false-positive 35S promoter screening results Eur Food Res Technol 210 367 372
37. www.agbios.com (2005)
38. www.biotech.org.cn (2005)
39. LT Yang JX Chen H Cheng SR Jia YH Liu LW Pan DB Zhang 2005a Validation of a cotton specific gene, Sad1, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic cottons Plant Cell Rep 24 237 245
40. LT Yang AH Pan JW Jia JY Ding JX Chen H Cheng CM Zhang DB Zhang 2005b Validation of a tomato specific gene, LAT52, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic tomatoes J Agric Food Chem 53 183 190
41. LT Yang JY Ding CM Zhang JW Jia HB Weng WX Liu DB Zhang 2005c Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR Plant Cell Rep 23 759 763