Generation of a stable mammalian cell line for simultaneous expression of multiple genes by using 2A peptide-based lentiviral vector

Biotechnology Letters

Volumn 31, Issue 3, 2009, Pages 353-359

  Hu, Tao ^a   Fu, Qiong ^a   Chen, Ping ^b   Zhang, Ke ^a   Guo, Deyin ^a  

^a WUHAN UNIVERSITY   (China)

^b ZHENGZHOU UNIVERSITY   (China)

Author keywords

2A Peptide; Co expression; Fluorescence activated cell sorting; Lentiviral vector; Mammalian cell line; Multiple genes

Indexed keywords

AMINES; ANIMAL CELL CULTURE; CELL CULTURE; CYTOLOGY; FLUORESCENCE; GENES; MAMMALS; PLASMA (HUMAN); PROTEINS; VECTORS;

2A PEPTIDE; CO-EXPRESSION; FLUORESCENCE ACTIVATED CELL SORTING; LENTIVIRAL VECTOR; MAMMALIAN CELL LINE; MULTIPLE GENES;

CELLS;

ENHANCED GREEN FLUORESCENT PROTEIN; FIREFLY LUCIFERASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN; RECOMBINANT PROTEIN; RED FLUORESCENT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CELL LINE; CULTURE TECHNIQUE; GENE EXPRESSION; GENE FUSION; GENE VECTOR; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; LENTIVIRINAE; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; REPORTER GENE;

AMINO ACID SEQUENCE; ARTIFICIAL GENE FUSION; BASE SEQUENCE; CELL CULTURE TECHNIQUES; CELL LINE; GENE EXPRESSION; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; LENTIVIRUS; LUCIFERASES, FIREFLY; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; RECOMBINANT PROTEINS;

LENTIVIRUS; MAMMALIA;

EID: 59149104555     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-008-9882-3     Document Type: Article

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