The calmodulin-ubiquitin (CUB) genes of Trypanosoma cruzi are essential for parasite viability

Molecular and Biochemical Parasitology

Volumn 78, Issue 1-2, 1996, Pages 217-225

  Ajioka, Janet ^a   Swindle, John ^a  

^a UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

Author keywords

Gene; Replacement essential genes; Transformation; Trypanosoma cruzi

Indexed keywords

CALMODULIN; UBIQUITIN;

ARTICLE; CELL VIABILITY; GENE DELETION; GENE EXPRESSION; GENE NUMBER; NONHUMAN; PRIORITY JOURNAL; TRYPANOSOMA CRUZI;

TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 0029952564     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(96)02627-8     Document Type: Article

References (21)

1. Swindle, J., Ajioka, J., Eisen, H., Sanwal, B., Jacquemot, C., Browder, Z. and Buck, G. (1988) The genomic organization and transcription of the ubiquitin genes of Trypanosoma cruzi. EMBO J. 7, 1121-1127.
2. Chung, S.H. and Swindle, J. (1990) Linkage of the calmodulin and ubiquitin loci in Trypanosoma cruzi. Nucleic Acids Res. 18, 4561-4569.
3. Ajioka, J. and Swindle, J. (1993) The calmodulin-ubiquitin associated genes of Trypanosoma cruzi: their identification and transcription. Mol. Biochem. Parasitol. 57, 127-136.
4. Wong, S., Kretsinger, R.H. and Campbell, D.A. (1992) Identification of a new EF-hand superfamily member from Trypanosoma brucei. Mol. Gen. Genet. 233, 225-230.
5. Swindle, J., Gillespie, R.D., Hariharan, S., Chung, S.H. and Ajioka, J. (1995) Gene replacements and genetics in Trypanosoma cruzi. In: Molecular Approaches to Parasitology (Boothroyd, J.C. and Komuniecki, R., ed.), Wiley-Liss, New York, Vol. 12, pp. 269-280.
6. Hariharan, S., Ajioka, J. and Swindle, J. (1993) Stable transformation of Trypanosoma cruzi: inactivation of the PUB12.5 polyubiquitin gene by targeted gene disruption. Mol. Biochem. Parasitol. 57, 15-30.
7. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
8. Gritz, L. and Davies, J. (1983) Plasmid-encoded hygromycin B resistance: sequence of the hygromycin B phosphotransferase gene and its expression in Escherichia coli and Sccharomyces cerevisiae. Gene 25, 179-188.
9. Gillespie, R.D., Ajioka, J. and Swindle, J. (1993) Using simultaneous, tandem gene replacements to study expression of the multicopy ubiquitin-fusion (FUS) gene family of Trypanosoma cruzi. Mol. Biochem. Parasitol. 60, 281-292.
10. Mullis, K.B. and Faloona, F. (1987) Specific synthesis of DNA in vitro via a polymerase catalyzed chain reaction. Methods Enzymol. 155, 335-350.
11. Southern, P.J. and Berg, P. (1982) Transformation of mammalian cells to antibiotic resistance with a bacterial gene under the control of the SV40 early region promoter. J. Mol. Appl. Genet. 1, 327-332.
12. Chung, S.H., Gillespie, R.D. and Swindle, J. (1994) Analyzing expression of the calmodulin and ubiquitin-fusion genes of Trypanosoma cruzi using simultaneous, independent dual gene replacements. Mol. Biochem. Parasitol. 63, 95-107.
13. Cross, G.A. and Takle, G.B. (1993) The surface transsialidase family of Trypanosoma cruzi. Annu. Rev. Microbiol. 47, 385-411.
14. Chung, S.H. (1993) Molecular Genetic Analysis of the Calmodulin Gene Family of Trypanosoma cruzi. University of Tennessee, Memphis, TN.
15. Peterson, D.S., Fouts, D.L. and Manning, J.E. (1989) The 85-kd surface antigen gene of Trypanosoma cruzi is telomeric and a member of a multigene family. EMBO J. 8, 3911-3916.
16. Tschudi, C., Young, A.S., Ruben, L., Patton, C.L. and Richards, F.F. (1985) Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5′ leader sequence. Proc. Natl. Acad. Sci. USA 82, 3998-4002.
17. Cooper, R., Inverso, J.A., Espinosa, M., Nogueira, N. and Cross, G.A. (1991) Characterization of a candidate gene for GP72, an insect stage-specific antigen of Trypanosoma cruzi. Mol. Biochem. Parasitol. 49, 45-59.
18. Erondu, N.E. and Donelson, J.E. (1991) Characterization of trypanosome protein phosphatase 1 and 2A catalytic subunits. Mol. Biochem. Parasitol. 49, 303-314.
19. Taylor, M.C., Kelly, J.M., Chapman, C.J., Fairlamb, A.H. and Miles, M.A. (1994) The structure, organization, and expression of the Leishmania donovani gene encoding trypanothione reductase. Mol Biochem. Parasitol. 64, 293-301.
20. Erondu, N.E. and Donelson, J.E. (1992) Differential expression of two mRNAs from a single gene encoding an HMG1-like DNA binding protein of African trypanosomes. Mol. Biochem. Parasitol. 51, 111-118.
21. Cruz, A.K., Titus, R. and Beverley, S.M. (1993) Plasticity in chromosome number and testing of essential genes in Leishmania by targeting. Proc. Natl. Acad. Sci. USA 90, 1599-1603.