Polymerase chain reaction-based gene disruption in Trypanosoma brucei

Molecular and Biochemical Parasitology

Volumn 87, Issue 1, 1997, Pages 113-115

  Gaud, Anne ^a   Carrington, Mark ^b   Deshusses, Jacques ^a   Schaller, Dominique R G ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b Department of Biochemistry ^*  (United Kingdom)

Author keywords

Gene disruption; Homologous recombination; Knockout; PCR; Trypanosoma brucei

Indexed keywords

ARTICLE; GENE DISRUPTION; GENETIC ANALYSIS; MICROBIAL GENETICS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; TRYPANOSOMA BRUCEI;

ANIMALS; CELLS, CULTURED; GENE TARGETING; GENES, PROTOZOAN; POLYMERASE CHAIN REACTION; TRYPANOSOMA BRUCEI BRUCEI;

TRYPANOSOMA; TRYPANOSOMA BRUCEI;

EID: 0030741031     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(97)00048-0     Document Type: Article

References (7)

1. A. Baudin, O. Ozier-Kalogeropoulos, A. Denouel, F. Lacroute, C. Culin, A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae, Nucleic Acids Res. 21 (1993) 3329-3330.
2. A. Wach, A. Brachat, R. Pohlmann, P. Philippsen, New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae, Yeast 10 (1994) 1793-1808.
3. A.L.M.A. ten Asbroek, C.A.A.M. Mol, R. Kieft, P. Borst, Stable transformation of Trypanosoma brucei, Mol. Biochem. Parasitol. 59 (1993) 133-142.
4. D.A. Parsell, Y. Sanchez, J.D. Stitzel, S. Lindquist, Hsp104 is a highly conserved protein with two essential nucleotide-binding sites, Nature 353 (1991) 270-273.
5. S. Gottesman, C. Squires, E. Pichersky, M. Carrington, M. Hobbs, J.S. Mattick, B. Dalrymple, H. Kuramitsu, T. Shiroza, T. Foster, W.P. Clark, B. Ross, C.L. Squires, M.R. Maurizi, Conservation of the regulatory subunit for the C1p ATP-dependent protease in prokaryotes and eukaryotes. GenBank accession number, M92325, Proc. Natl. Acad. Sci. USA 87 (1990) 3513-3517.
6. R. Brun, M. Schonenberger, Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei, Acta Trop. 36 (1979) 289-292.
7. J.C.B.M. Zomerdijk, M. Ouellette, A.L.M.A. ten Asbroek, R. Kieft, A.M.M. Bommer, C.E. Clayton, P. Borst, The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei, EMBO J. 8 (1990) 2791-2801.