Simple one-week method to construct gene-targeting vectors: Application to production of human knockout cell lines

BioTechniques

Volumn 41, Issue 3, 2006, Pages 311-316

  Iiizumi, Susumu ^a   Nomura, Yuji ^a   So, Sairei ^a   Uegaki, Koichi ^a   Aoki, Kayoko ^a   Shibahara, Kei Ichi ^b   Adachi, Noritaka ^a   Koyama, Hideki ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF GENETICS   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL CULTURE; DISEASES; GENES; MUTAGENESIS;

CELL LINES; EMBRYONIC STEM (ES) CELLS; GENOMIC FRAGMENTS; MUTATIONS;

CLONING;

ARTICLE; B LYMPHOCYTE; EMBRYONIC STEM CELL; GENE AMPLIFICATION; GENE DISRUPTION; GENE FUNCTION; GENE LOCUS; GENE TARGETING; GENE VECTOR; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; KNOCKOUT GENE; POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; BIOLOGICAL MODEL; CELL LINE; CHEMISTRY; CYTOLOGY; EMBRYO; GENETIC PROCEDURES; GENETICS; GENOME; HOMOZYGOTE; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; STEM CELL;

ANIMALIA; MAMMALIA;

POLYDEOXYRIBONUCLEOTIDE SYNTHASE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE (ATP); PRIMER DNA;

BASE SEQUENCE; CELL LINE; DNA LIGASES; DNA PRIMERS; EMBRYO; GENE TARGETING; GENETIC TECHNIQUES; GENETIC VECTORS; GENOME; HOMOZYGOTE; HUMANS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; STEM CELLS;

EID: 34047223844     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112233     Document Type: Article

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