Liver microsomes and S9 from rainbow trout (oncorhynchus mykiss): Comparison of basal-level enzyme activities with rat and determination of xenobiotic intrinsic clearance in support of bioaccumulation assessment

Environmental Toxicology and Chemistry

Volumn 28, Issue 3, 2009, Pages 481-488

  Han, Xing ^a   Nabb, Diane L ^a   Yang, Ching Hui ^a   Snajdr, Suzanne I ^a   Mingoia, Robert T ^a  

^a DUPONT COMPANY   (United States)

Author keywords

Bioaccumulation assessment; Bioconcentration factor; Trout liver microsomes; Trout liver s9

Indexed keywords

BIOCONCENTRATION FACTOR; GLUTATHIONE-S-TRANSFERASE; HEPATOCYTES; HYDROXYLASES; IN-VITRO; LIVER MICROSOMES; ONCORHYNCHUS MYKISS; PER UNIT; PROTEIN CONTENTS; RAINBOW TROUT; RAT LIVER MICROSOMES; REFERENCE COMPOUNDS; SCALING FACTORS; STANDARD DEVIATION; TROUT LIVER MICROSOMES; TROUT LIVER S9;

BIOACCUMULATION; BIOCHEMISTRY; ENVIRONMENTAL IMPACT; FORECASTING;

LIVER;

CYTOCHROME P450 3A4; ETHOXYRESORUFIN DEETHYLASE; XENOBIOTIC AGENT; CELL EXTRACT;

BIOACCUMULATION; BIOLOGICAL ANALYSIS; ENZYME ACTIVITY; FISH; METABOLISM; NUMERICAL MODEL; PROTEIN; XENOBIOTICS;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOACCUMULATION; CONTROLLED STUDY; ENZYME ACTIVITY; IN VITRO STUDY; IN VIVO STUDY; LIVER CELL; LIVER CLEARANCE; LIVER MICROSOME; MALE; NONHUMAN; PREDICTION; PRIORITY JOURNAL; PROTEIN CONTENT; RAINBOW TROUT; RAT; XENOBIOTIC METABOLISM; ANIMAL; BIOASSAY; CHEMISTRY; COMPARATIVE STUDY; CULTURE MEDIUM; ENVIRONMENTAL MONITORING; METABOLISM; POLLUTANT;

ONCORHYNCHUS MYKISS; RATTUS; SALMONIDAE;

ANIMALS; BIOLOGICAL ASSAY; CELL EXTRACTS; CULTURE MEDIA; ENVIRONMENTAL MONITORING; ENVIRONMENTAL POLLUTANTS; HEPATOCYTES; MALE; MICROSOMES, LIVER; ONCORHYNCHUS MYKISS; RATS; XENOBIOTICS;

EID: 66649121198     PISSN: 07307268     EISSN: 15528618     Source Type: Journal    
DOI: 10.1897/08-269.1     Document Type: Article

References (46)

1. Weisbrod AV, Burkhard LP, Arnot J, Mekenyan O, Howard PH, Russom C, Boethling R, Sakuratani Y, Traas T, Bridges T, Lutz C, Bonneil M, Woodburn K, Parkerton T. 2007 Workgroup report: Review of fish bioaccumulation databases used to identify persistent, bioaccumulative, toxic substances Environ Health Perspect 115: 255-261.
2. Nichols JW, Fitzsimmons PN, Burkhard LP. 2007 In vitro-in vivo extrapolation of quantitative hepatic biotransformation data for fish. II. Modeled effects on chemical bioaccumulation Environ Toxicol Chem. 26: 1304-1319.
3. Han X, Nabb DL, Mingoia RT, Yang CH. 2007 Determination of xenobiotic intrinsic clearance in freshly isolated hepatocytes from rainbow trout (Oncorhynchus mykiss) and rat and its application in bioaccumulation assessment Environ Sci Technol 41: 3269-3276.
4. Arnot JA, Gobas FA. 2004 A food web bioaccumulation model for organic chemicals in aquatic ecosystems Environ Toxicol Chem 23: 2343-2355.
5. Han X, Nabb DL, Yang CH, Snajdr SI, Hoke RA, Mingoia RT. 2008 Xenobiotic intrinsic clearance in freshly isolated hepatocytes from rainbow trout (Oncorhynchus mykiss): Determination of trout hepatocellularity, optimization of cell concentrations and comparison of serum and serum-free incubations Aquat Toxicol 89: 11-17.
6. Mohutsky MA, Chien JY, Ring BJ, Wrighton SA. 2006 Predictions of the in vivo clearance of drugs from rate of loss using human liver microsomes for phase I and phase II biotransformations Pharm Res 23: 654-662.
7. Austin RP, Barton P, Cockroft SL, Wenlock MC, Riley RJ. 2002 The influence of nonspecific microsomal binding on apparent intrinsic clearance, and its prediction from physicochemical properties Drug Metab Dispos 30: 1497-1503.
8. Carlile DJ, Hakooz N, Bayliss MK, Houston JB. 1999 Microsomal prediction of in vivo clearance of CYP2C9 substrates in humans Br J Clin Pharmacol 47: 625-635.
9. Naritomi Y, Terashita S, Kimura S, Suzuki A, Kagayama A, Sugiyama Y. 2001 Prediction of human hepatic clearance from in vivo animal experiments and in vitro metabolic studies with liver microsomes from animals and humans Drug Metab Dispos 29: 1316-1324.
10. Obach RS. 1999 Prediction of human clearance of twenty-nine drugs from hepatic microsomal intrinsic clearance data: An examination of in vitro half-life approach and nonspecific binding to microsomes Drug Metab Dispos 27: 1350-1359.
11. Brown HS, Griffin M, Houston JB. 2007 Evaluation of cryopreserved human hepatocytes as an alternative in vitro system to microsomes for the prediction of metabolic clearance Drug Metab Dispos 35: 293-301.
12. Ito K, Houston JB. 2005 Prediction of human drug clearance from in vitro and preclinical data using physiologically based and empirical approaches Pharm Res 22: 103-112.
13. Cowan-Ellsberry CE, Dyer SD, Erhardt S, Bernhard MJ, Roe AL, Dowty ME, Weisbrod AV 2008 Approach for extrapolating in vitro metabolism data to refine bioconcentration factor estimates Chemosphere 70: 1804-1817.
14. Statham CN, Szyjka SP, Menahan LA, Lech JJ. 1977 Fractionation and subcellular localization of marker enzymes in rainbow trout liver Biochem Pharmacol 26: 1395-1400.
15. Nichols JW, Schultz IR, Fitzsimmons PN. 2006 In vitro-in vivo extrapolation of quantitative hepatic biotransformation data for fish. I. A review of methods, and strategies for incorporating intrinsic clearance estimates into chemical kinetic models Aquat Toxicol 78: 74-90.
16. Nabb DL, Mingoia RT, Yang CH, Han X. 2006 Comparison of basal level metabolic enzyme activities of freshly isolated hepatocytes from rainbow trout (Oncorhynchus mykiss) and rat Aquat Toxicol 80: 52-59.
17. Castren M, Oikari A. 1983 Optimal assay conditions for liver UDP-glucuronosyltransferase from the rainbow trout, Salmo gairdneri Comp Biochem Physiol C Toxicol Pharmacol 76: 365-369.
18. Bock KW, Burchell B, Dutton GJ, Hanninen O, Mulder GJ, Owens IS, Siest G, Tephly TR. 1983 UDP-glucuronosyltransferase activities. Guidelines for consistent interim terminology and assay conditions Biochem Pharmacol 32: 953-955.
19. Habig WH, Pabst MJ, Jakoby WB. 1974 Glutathiones-transferases. The first enzymatic step in mercapturic acid formation J Biol Chem. 249: 7130-7139.
20. Jones HM, Houston JB. 2004 Substrate depletion approach for determining in vitro metabolic clearance: Time dependencies in hepatocyte and microsomal incubations Drug Metab Dispos 32: 973-982.
21. Nichols JW, McKim JM, Andersen ME, Gargas ML, Clewell HJ III, Erickson RJ. 1990 A physiologically based toxicokinetic model for the uptake and disposition of waterborne organic chemicals in fish Toxicol Appl Pharmacol 106: 433-447.
22. Arnot JA, Gobas FA. 2003 A generic QSAR for assessing the bioaccumulation potential of organic chemicals in aquatic food webs QSAR & Combinatorial Science 22: 337-345.
23. De Buck SS, Sinha VK, Fenu LA, Nijsen MJ, Mackie CE, Gilissen RA. 2007 Prediction of human, pharmacokinetics using physiologically based modeling: A. retrospective analysis of 26 clinically tested drugs Drug Metab Dispos 35: 1766-1780.
24. Sohlenius-Sternbeck AK. 2006 Determination of the hepatocellularity number for human, dog, rabbit, rat and mouse livers from protein concentration measurements Toxicol In Vitro 20: 1582-1586.
25. Foti RS, Fisher MB. 2004 Impact of incubation conditions on bufuralol human clearance predictions: Enzyme lability and nonspecific binding Drug Metab Dispos 32: 295-304.
26. Obach RS. 1996 The importance of nonspecific binding in in vitro matrices, its impact on enzyme kinetic studies of drug metabolism reactions, and implications for in vitro-in vivo correlations Drug Metab Dispos 24: 1047-1049.
27. Obach RS. 1997 Nonspecific binding to microsomes: impact on scale-up of in vitro intrinsic clearance to hepatic clearance as assessed through examination of warfarin, imipramine, and propranolol Drug Metab Dispos 25: 1359-1369.
28. Riley RJ, McGinnity DF, Austin RP. 2005 A unified model for predicting human hepatic, metabolic clearance from in vitro intrinsic clearance data in hepatocytes and microsomes Drug Metab Dispos 33: 1304-1311.
29. Rowland Yeo K, Howgate EM, Tucker GT, Rostami-Hodjegan A. 2004 Predicting the clearance of CYP2C9 substrates Drug Metab Dispos 32: 1522 (author reply: 1523).
30. Kalvass JC, Tess DA, Giragossian C, Linhares MC, Maurer TS. 2001 Influence of microsomal concentration on apparent intrinsic clearance: Implications for scaling in vitro data Drug Metab Dispos 29: 1332-1336.
31. Grime K, Riley RJ. 2006 The impact of in vitro binding on in vitro-in vivo extrapolations, projections of metabolic clearance and clinical drug-drug interactions Curr Drug Metab 7: 251-264.
32. Gertz M, Kilford PJ, Houston JB, Galetin A. 2008 Drug lipophilicity and microsomal protein concentration as determinants in the prediction of the fraction unbound in microsomal incubations Drug Metab Dispos 36: 535-542.
33. Kilford PJ, Gertz M, Houston JB, Galetin A. 2008 Hepatocellular binding of drugs: Correction for unbound fraction in hepatocyte incubations using microsomal binding or drug lipophilicity data Drug Metab Dispos 36: 1194-1197.
34. Lauren DJ, Halarnkar PP, Hammcock BD, Hinton DE. 1989 Microsomal and cytosolic epoxide hydrolase and glutathione-S-transferase activities in the gill, liver, and kidney of the rainbow trout, Salmo gairdneri. Baseline levels and optimization of assay conditions Biochem Pharmacol 38: 881-887.
35. Casalino E, Sblano C, Landriscina V, Calzaretti G, Landriscina C. 2004 Rat liver glutathione-5-transferase activity stimulation following acute cadmium or manganese intoxication Toxicology 200: 29-38.
36. Lundqvist G, Morgenstern R. 1992 Studies on the activation of rat liver microsomal glutathione transferase in isolated hepatocytes Biochem Pharmacol 43: 131-135.
37. Oesch F, Wolf CR. 1989 Properties of the microsomal and cytosolic glutathione transferases involved in hexachloro-1:3-butadiene conjugation Biochem Pharmacol 38: 353-359.
38. Baars AJ, Jansen M, Breimer DD. 1978 The influence of phenobarbital, 3-methylcholanthrene and 2,3,7,8-tetrachlorodibenzo-p-dioxin on glutathione-5-transferase activity of rat liver cytosol Biochem. Pharmacol 27: 2487-2497.
39. Fisher MB, Campanale K, Ackermann BL, VandenBranden M, Wrighton SA. 2000 In vitro glucuronidation using human liver microsomes and the pore-forming peptide alamethicin Drug Metab Dispos 28: 560-566.
40. Fulceri R, Banhegyi G, Gamberucci A, Giunti R, Mandl J, Benedetti A. 1994 Evidence for the intraluminal positioning of pnitrophenol UDP- glucuronosyltransferase activity in rat liver microsomal vesicles Arch Biochem Biophys 309: 43-46.
41. Lett E, Kriszt W, de Sandro V, Ducrotoy G, Richert L. 1992 Optimal detergent activation of rat liver microsomal UDP-glucuronosyl transferases toward morphine and 1-naphthol: Contribution to induction and latency studies Biochem Pharmacol 43: 1649-1653.
42. Burchell B, Coughtrie MW 1989 UDP-glucuronosyltransferases Pharmacol Ther 43: 261-289.
43. Faber KN, Muller M, Jansen PL. 2003 Drug transport proteins in the liver Adv Drug Delivery Rev 55: 107-124.
44. Ito K, Houston JB. 2004 Comparison of the use of liver models for predicting drug clearance using in vitro kinetic data from hepatic microsomes and isolated hepatocytes Pharm Res 21: 785-792.
45. Rawden HC, Carlile DJ, Tindall A, Hallifax D, Galetin A, Ito K, Houston JB. 2005 Microsomal prediction of in vivo clearance and associated interindividual variability of six benzodiazepines in humans Xenobiotica 35: 603-625.
46. Parkerton. TF, Arnot JA, Weisbrod AV, Russom C, Hoke RA, Woodburn K, Traas T, Bunnell M, Burkhard LP, Lampit MA. 2008 Guidance for evaluating in vivo fish bioaccumulation data Integr Environ Assess Manag 4: 139-155.