Xenobiotic intrinsic clearance in freshly isolated hepatocytes from rainbow trout (Oncorhynchus mykiss): Determination of trout hepatocellularity, optimization of cell concentrations and comparison of serum and serum-free incubations

Aquatic Toxicology

Volumn 89, Issue 1, 2008, Pages 11-17

  Han, Xing ^a   Mingoia, Robert T ^a   Nabb, Diane L ^a   Yang, Ching Hui ^a   Snajdr, Suzanne I ^a   Hoke, Robert A ^a  

^a DUPONT COMPANY   (United States)

Author keywords

Bioaccumulation assessment; Bioconcentration factor; Hepatic clearance; Hepatocellularity; In vitro prediction; Trout hepatocytes

Indexed keywords

2,4 DI(TERT BUTYL)PHENOL; 4 NONYLPHENOL; 4,4 BIS(DIMETHYLAMINO)BENZOPHENONE; BENZO[A]PYRENE; MOLINATE; XENOBIOTIC AGENT;

ASSESSMENT METHOD; BIOACCUMULATION; CELL ORGANELLE; COMPARATIVE STUDY; METABOLISM; OPTIMIZATION; PLASMA; POLLUTION EFFECT; SALMONID; SERUM; XENOBIOTICS;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BIOACCUMULATION; CELL ISOLATION; CELL METABOLISM; CONTROLLED STUDY; IN VITRO STUDY; INCUBATION TIME; LIVER CELL; LIVER CLEARANCE; MALE; NONHUMAN; PRIORITY JOURNAL; RAINBOW TROUT;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; CULTURE MEDIA; HEPATOCYTES; MALE; ONCORHYNCHUS MYKISS;

ONCORHYNCHUS MYKISS; RATTUS; SALMONIDAE;

EID: 47149086363     PISSN: 0166445X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aquatox.2008.05.010     Document Type: Article

References (27)

1. Arnot J.A., and Gobas F.A. A generic QSAR for assessing the bioaccumulation potential of organic chemicals in aquatic food webs. QSAR Comb. Sci. 22 (2003) 337-345
2. Arnot J.A., and Gobas F.A. A food web bioaccumulation model for organic chemicals in aquatic ecosystems. Environ. Toxicol. Chem. 23 (2004) 2343-2355
3. Bachmann K., Byers J., and Ghosh R. Prediction of in vivo hepatic clearance from in vitro data using cryopreserved human hepatocytes. Xenobiotica 33 (2003) 475-483
4. Blanchard N., Richert L., Notter B., Delobel F., David P., Coassolo P., and Lave T. Impact of serum on clearance predictions obtained from suspensions and primary cultures of rat hepatocytes. Eur. J. Pharm. Sci. 23 (2004) 189-199
5. Blanchard N., Alexandre E., Abadie C., Lave T., Heyd B., Mantion G., Jaeck D., Richert L., and Coassolo P. Comparison of clearance predictions using primary cultures and suspensions of human hepatocytes. Xenobiotica 35 (2005) 1-15
6. Blanchard N., Hewitt N.J., Silber P., Jones H., Coassolo P., and Lave T. Prediction of hepatic clearance using cryopreserved human hepatocytes: a comparison of serum and serum-free incubations. J. Pharm. Pharmacol. 58 (2006) 633-641
7. Grime K., and Riley R.J. The impact of in vitro binding on in vitro-in vivo extrapolations, projections of metabolic clearance and clinical drug-drug interactions. Curr. Drug Metab. 7 (2006) 251-264
8. Hampton J.A., Lantz R.C., and Hinton D.E. Functional units in rainbow trout (Salmo gairdneri, Richardson) liver: III. Morphometric analysis of parenchyma, stroma, and component cell types. Am. J. Anat. 185 (1989) 58-73
9. Han X., Nabb D.L., Mingoia R.T., and Yang C.H. Determination of xenobiotic intrinsic clearance in freshly isolated hepatocytes from rainbow trout (Oncorhynchus mykiss) and rat and its application in bioaccumulation assessment. Environ. Sci. Technol. 41 (2007) 3269-3276
10. Houston J.B. Utility of in vitro drug metabolism data in predicting in vivo metabolic clearance. Biochem. Pharmacol. 47 (1994) 1469-1479
11. Jones H.M., and Houston J.B. Substrate depletion approach for determining in vitro metabolic clearance: time dependencies in hepatocyte and microsomal incubations. Drug Metab. Dispos. 32 (2004) 973-982
12. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
13. Ludden L.K., Ludden T.M., Collins J.M., Pentikis H.S., and Strong J.M. Effect of albumin on the estimation, in vitro, of phenytoin Vmax and Km values: implications for clinical correlation. J. Pharmacol. Exp. Ther. 282 (1997) 391-396
14. Nabb D.L., Mingoia R.T., Yang C.H., and Han X. Comparison of basal level metabolic enzyme activities of freshly isolated hepatocytes from rainbow trout (Oncorhynchus mykiss) and rat. Aquat. Toxicol. 80 (2006) 52-59
15. Nichols J.W., McKim J.M., Andersen M.E., Gargas M.L., Clewell III H.J., and Erickson R.J. A physiologically based toxicokinetic model for the uptake and disposition of waterborne organic chemicals in fish. Toxicol. Appl. Pharmacol. 106 (1990) 433-447
16. Nichols J.W., Schultz I.R., and Fitzsimmons P.N. In vitro-in vivo extrapolation of quantitative hepatic biotransformation data for fish I. A review of methods, and strategies for incorporating intrinsic clearance estimates into chemical kinetic models. Aquat. Toxicol. 78 (2006) 74-90
17. Nichols J.W., Fitzsimmons P.N., and Burkhard L.P. In vitro-in vivo extrapolation of quantitative hepatic biotransformation data for fish II. Modeled effects on chemical bioaccumulation. Environ. Toxicol. Chem. 26 (2007) 1304-1319
18. Obach R.S. The importance of nonspecific binding in in vitro matrices, its impact on enzyme kinetic studies of drug metabolism reactions, and implications for in vitro-in vivo correlations. Drug Metab. Dispos. 24 (1996) 1047-1049
19. Obach R.S. Nonspecific binding to microsomes: impact on scale-up of in vitro intrinsic clearance to hepatic clearance as assessed through examination of warfarin, imipramine, and propranolol. Drug Metab. Dispos. 25 (1997) 1359-1369
20. Riley R.J., McGinnity D.F., and Austin R.P. A unified model for predicting human hepatic, metabolic clearance from in vitro intrinsic clearance data in hepatocytes and microsomes. Drug Metab. Dispos. 33 (2005) 1304-1311
21. Rowland Yeo K., Howgate E.M., Tucker G.T., and Rostami-Hodjegan A. Predicting the clearance of CYP2C9 substrates. Drug Metab. Dispos. 32 (2004) 1522-1523 author reply
22. Shibata Y., Takahashi H., and Ishii Y. A convenient in vitro screening method for predicting in vivo drug metabolic clearance using isolated hepatocytes suspended in serum. Drug Metab. Dispos. 28 (2000) 1518-1523
23. Shibata Y., Takahashi H., Chiba M., and Ishii Y. Prediction of hepatic clearance and availability by cryopreserved human hepatocytes: an application of serum incubation method. Drug Metab. Dispos. 30 (2002) 892-896
24. Soars M.G., McGinnity D.F., Grime K., and Riley R.J. The pivotal role of hepatocytes in drug discovery. Chem. Biol. Interact. 168 (2007) 2-15
25. Sohlenius-Sternbeck A.K. Determination of the hepatocellularity number for human, dog, rabbit, rat and mouse livers from protein concentration measurements. Toxicol. In Vitro 20 (2006) 1582-1586
26. Weisbrod A.V., Burkhard L.P., Arnot J., Mekenyan O., Howard P.H., Russom C., Boethling R., Sakuratani Y., Traas T., Bridges T., Lutz C., Bonnell M., Woodburn K., and Parkerton T. Workgroup report: review of fish bioaccumulation databases used to identify persistent, bioaccumulative, toxic substances. Environ. Health Perspect. 115 (2007) 255-261
27. Worboys P.D., Bradbury A., and Houston J.B. Kinetics of drug metabolism in rat liver slices II. Comparison of clearance by liver slices and freshly isolated hepatocytes. Drug Metab. Dispos. 24 (1996) 676-681