Comparison of basal level metabolic enzyme activities of freshly isolated hepatocytes from rainbow trout (Oncorhynchus mykiss) and rat

Aquatic Toxicology

Volumn 80, Issue 1, 2006, Pages 52-59

  Nabb, Diane L ^a   Mingoia, Robert T ^a   Yang, Ching Hui ^a   Han, Xing ^a  

^a DUPONT COMPANY   (United States)

Author keywords

Bioaccumulation; Biotransformation; Cytochrome P450; Enzyme activity; Trout hepatocytes

Indexed keywords

7 METHOXYRESORUFIN O DEALKYLASE; CELL PROTEIN; CHLORZOXAZONE 6 HYDROXYLASE; CYTOCHROME P450; CYTOCHROME P450 1A; CYTOCHROME P450 3A4; ENZYME; ETHOXYRESORUFIN DEETHYLASE; GLUTATHIONE TRANSFERASE; LAURIC ACID 11 HYDROXYLASE; LAURIC ACID DERIVATIVE; PENTOXYRESORUFIN DEALKYLASE; UNCLASSIFIED DRUG; XENOBIOTIC AGENT;

BIOACCUMULATION; BIOTRANSFORMATION; COMPARATIVE STUDY; DETOXIFICATION; ENZYME ACTIVITY; RODENT; SALMONID; XENOBIOTICS;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BIOTRANSFORMATION; CELL ISOLATION; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; LIVER CELL; METABOLIC REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN BLOOD LEVEL; RAINBOW TROUT; RAT;

ANIMALS; BIOTRANSFORMATION; CYTOCHROME P-450 ENZYME SYSTEM; GLUTATHIONE TRANSFERASE; HEPATOCYTES; MALE; ONCORHYNCHUS MYKISS; RATS; RATS, SPRAGUE-DAWLEY; XENOBIOTICS;

MAMMALIA; ONCORHYNCHUS MYKISS; SALMONIDAE;

EID: 33748784049     PISSN: 0166445X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.aquatox.2006.07.012     Document Type: Article

References (47)

1. Andersson T., and Koivusaari U. Oxidative and conjugative metabolism of xenobiotics in isolated liver cells from thermally acclimated rainbow trout. Aquat. Toxicol. 8 (1986) 85-92
2. Andersson T., Pesonen M., and Johansson C. Differential induction of cytochrome P-450-dependent monooxygenase, epoxide hydrolase, glutathione transferase and UDP glucuronosyl transferase activities in the liver of the rainbow trout by beta-naphthoflavone or Clophen A50. Biochem. Pharmacol. 34 (1985) 3309-3314
3. Arnot J.A., and Gobas F.A. A food web bioaccumulation model for organic chemicals in aquatic ecosystems. Environ. Toxicol. Chem. 23 (2004) 2343-2355
4. Bars R.G., Mitchell A.M., Wolf C.R., and Elcombe C.R. Induction of cytochrome P-450 in cultured rat hepatocytes. The heterogeneous localization of specific isoenzymes using immunocytochemistry. Biochem. J. 262 (1989) 151-158
5. Bauermeister A., Lewendon A., Ramage P.I., and Nimmo I.A. Distribution and some properties of the glutathione S-transferase and gamma-glutamyl transpeptidase activities of rainbow trout. Comp. Biochem. Physiol. C 74 (1983) 89-93
6. Bogaards J.J., Bertrand M., Jackson P., Oudshoorn M.J., Weaver R.J., van Bladeren P.J., and Walther B. Determining the best animal model for human cytochrome P450 activities: a comparison of mouse, rat, rabbit, dog, micropig, monkey and man. Xenobiotica 30 (2000) 1131-1152
7. Buhler D.R., Miranda C.L., Deinzer M.L., Griffin D.A., and Henderson M.C. The regiospecific hydroxylation of lauric acid by rainbow trout (Oncorhynchus mykiss) cytochrome P450s. Drug Metab. Dispos. 25 (1997) 1176-1183
8. Buhler D.R., and Wang-Buhler J.L. Rainbow trout cytochrome P450s: purification, molecular aspects, metabolic activity, induction and role in environmental monitoring. Comp. Biochem. Physiol. C: Pharmacol. Toxicol. Endocrinol. 121 (1998) 107-137
9. Burke M.D., and Mayer R.T. Ethoxyresoruf: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene. Drug Metab. Dispos. 2 (1974) 583-588
10. De-Oliveira A.C., Ribeiro-Pinto L.F., and Paumgartten J.R. In vitro inhibition of CYP2B1 monooxygenase by beta-myrcene and other monoterpenoid compounds. Toxicol. Lett. 92 (1997) 39-46
11. Dimitrov S., Dimitrova N., Parkerton T., Comber M., Bonnell M., and Mekenyan O. Base-line model for identifying the bioaccumulation potential of chemicals. SAR QSAR Environ. Res. 16 (2005) 531-554
12. Donato M.T., Viitala P., Rodriguez-Antona C., Lindfors A., Castell J.V., Raunio H., Gomez-Lechon M.J., and Pelkonen O. CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers. Drug Metab. Dispos. 28 (2000) 1321-1326
13. Furimsky M., Moon T.W., and Perry S.F. Evidence for the role of a Na(+)/HCO(3)(-) cotransporter in trout hepatocyte pHi regulation. J. Exp. Biol. 203 (2000) 2201-2208
14. Gebhardt R., Hengstler J.G., Muller D., Glockner R., Buenning P., Laube B., Schmelzer E., Ullrich M., Utesch D., Hewitt N., Ringel M., Hilz B.R., Bader A., Langsch A., Koose T., Burger H.J., Maas J., and Oesch F. New hepatocyte in vitro systems for drug metabolism: metabolic capacity and recommendations for application in basic research and drug development, standard operation procedures. Drug Metab. Rev. 35 (2003) 145-213
15. George S.G. Enzymology and molecular biology of phase II xenobiotic-conjugating enzymes in fish. In: Malins D.C., and Ostrander G.K. (Eds). Aquatic Toxicology: Molecular, Biochemical and Cellular Perspectives (1994), Lewis Publisher, CRC press 37-85
16. Gonzalez F.J. Molecular genetics of the P-450 superfamily. Pharmacol. Ther. 45 (1990) 1-38
17. Habig W.H., Pabst M.J., and Jakoby W.B. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J. Biol. Chem. 249 (1974) 7130-7139
18. Hanioka N., Tatarazako N., Jinno H., Arizono K., and Ando M. Determination of cytochrome P450 1A activities in mammalian liver microsomes by high-performance liquid chromatography with fluorescence detection. J. Chromatogr. B: Biomed. Sci. Appl. 744 (2000) 399-406
19. Hengstler J.G., Ringel M., Biefang K., Hammel S., Milbert U., Gerl M., Klebach M., Diener B., Platt K.L., Bottger T., Steinberg P., and Oesch F. Cultures with cryopreserved hepatocytes: applicability for studies of enzyme induction. Chem. Biol. Interact. 125 (2000) 51-73
20. Iwata H., Yoshinari K., Negishi M., and Stegeman J.J. Species-specific responses of constitutively active receptor (CAR)-CYP2B coupling: lack of CYP2B inducer-responsive nuclear translocation of CAR in marine teleost, scup (Stenotomus chrysops). Comp. Biochem. Physiol. C: Toxicol. Pharmacol. 131 (2002) 501-510
21. Klaunig J.E., Ruch R.J., and Goldblatt P.J. Trout hepatocyte culture: isolation and primary culture. In Vitro Cell. Dev. Biol. 21 (1985) 221-228
22. Kramer H.J., van den Berg M., Delang R.J., Brandsma L., and Dejongh J. Biotransformation rates of Ugilec 141 (tetrachlorobenzyltoluenes) in rat and trout microsomes. Chemosphere 40 (2000) 1283-1288
23. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265-275
24. Miyamoto M., Saito S., Takimoto Y., and Matsuo M. Effect of metabolism on bioconcentration of geometric isomers of d-phenothrin in fish. Chemosphere 24 (1992) 2001-2007
25. Namkung M.J., Yang H.L., Hulla J.E., and Juchau M.R. On the substrate specificity of cytochrome P450IIIA1. Mol. Pharmacol. 34 (1988) 628-637
26. Nerurkar P.V., Park S.S., Thomas P.E., Nims R.W., and Lubet R.A. Methoxyresorufin and benzyloxyresoruf: substrates preferentially metabolized by cytochromes P4501A2 and 2B, respectively, in the rat and mouse. Biochem. Pharmacol. 46 (1993) 933-943
27. Nichols J.W., Schultz I.R., and Fitzsimmons P.N. In vitro-in vivo extrapolation of quantitative hepatic biotransformation data for fish. I. A review of methods, and strategies for incorporating intrinsic clearance estimates into chemical kinetic models. Aquat. Toxicol. 78 (2006) 74-90
28. Oesch F., Wagner H., Platt K.L., and Arand M. Improved sample preparation for the testosterone hydroxylation assay using disposable extraction columns. J. Chromatogr. 582 (1992) 232-235
29. Pedersen R.T., and Hill E.M. Biotransformation of the xenoestrogen 4-tert-octylphenol in hepatocytes of rainbow trout (Oncorhynchus mykiss). Xenobiotica 30 (2000) 867-879
30. Peter R., Bocker R., Beaune P.H., Iwasaki M., Guengerich F.P., and Yang C.S. Hydroxylation of chlorzoxazone as a specific probe for human liver cytochrome P-450IIE1. Chem. Res. Toxicol. 3 (1990) 566-573
31. Pohl R.J., and Fouts J.R. A rapid method for assaying the metabolism of 7-ethoxyresorufin by microsomal subcellular fractions. Anal. Biochem. 107 (1980) 150-155
32. Powell P.K., Wolf I., and Lasker J.M. Identification of CYP4A11 as the major lauric acid omega-hydroxylase in human liver microsomes. Arch. Biochem. Biophys. 335 (1996) 219-226
33. Quistorff B., Dich J., and Grunnet N. Preparation of isolated rat liver hepatocytes. In: Pollard J.W., and Walker J.M. (Eds). Methods in Molecular Biology: Animal Cell Culture, vol. 5 (1989), Humana Press, Clifton, New Jersey 151-160
34. Ringel M., Oesch F., Gerl M., Klebach M., Quint M., Bader A., Bottger T., and Hengstler J.G. Permissive and suppressive effects of dexamethasone on enzyme induction in hepatocyte co-cultures. Xenobiotica 32 (2002) 653-666
35. Ronis M.J., and Walker C.H. Species variations in the metabolism of liposoluble organochlorine compounds by hepatic microsomal monooxygenase: comparative kinetics in four vertebrate species. Comp. Biochem. Physiol. C 82 (1985) 445-449
36. Schultz I.R., and Hayton W.L. Interspecies scaling of the bioaccumulation of lipophilic xenobiotics in fish: an example using trifluralin. Environ. Toxicol. Chem. 18 (1999) 1440-1449
37. Starcevic S.L., and Zielinski B.S. Glutathione and glutathione S-transferase in the rainbow trout olfactory mucosa during retrograde degeneration and regeneration of the olfactory nerve. Exp. Neurol. 146 (1997) 331-340
38. Stegeman J.J., and Hahn M.E. Biochemistry and molecular biology of monooxygenase current perspective on forms, functions, and regulation of cytochrome P450 in aquatic species. In: Malins D.C., and Ostrander G.K. (Eds). Aquatic Toxicology: Molecular, Biochemical, and Cellular Perspectives (1994), CRC press, Boca Raton 87-206
39. Steward A.R., Elmarakby S.A., Stuart K.G., Kumar S., and Sikka H.C. Metabolism of 2-acetylaminofluorene by hepatocytes isolated from rainbow trout. Toxicol. Appl. Pharmacol. 130 (1995) 188-196
40. Stuchal, L.D., Kleinow, K.M., Stegeman, J.J., James, M.O., in press. Demethylation of the pesticide methoxychlor in liver and intestine from untreated, methoxychlor-treated and 3-methylcholanthrene-treated channel catfish (Ictalurus punctatus): evidence for roles of CYP1 and 3A family isozymes. Drug Metab. Dispos.
41. van der Oost R., Beyer J., and Vermeulen N.P.E. Fish bioaccumulation and biomarkers in environmental risk assessment: a review. Environ. Toxicol. Pharmacol. 13 (2003) 57-149
42. Wallace K.B., and Dargan J.E. Intrinsic metabolic clearance of parathion and paraoxon by livers from fish and rodents. Toxicol. Appl. Pharmacol. 90 (1987) 235-242
43. Waxman D.J., Attisano C., Guengerich F.P., and Lapenson D.P. Human liver microsomal steroid metabolism: identification of the major microsomal steroid hormone 6 beta-hydroxylase cytochrome P-450 enzyme. Arch. Biochem. Biophys. 263 (1988) 424-436
44. Whyte J.J., Jung R.E., Schmitt C.J., and Tillitt D.E. Ethoxyresorufin-O-deethylase (EROD) activity in fish as a biomarker of chemical exposure. Crit. Rev. Toxicol. 30 (2000) 347-570
45. Wikie M.P., Simmons H.E., and Wood C.M. Physiological adaptations of rainbow trout to chronically elevated water pH (pH = 9.5). J. Exp. Zool. 274 (1996) 1-14
46. Williams D.E., Becker R.R., Potter D.W., Guengerich F.P., and Buhler D.R. Purification and comparative properties of NADPH-cytochrome P-450 reductase from rat and rainbow trout: differences in temperature optima between reconstituted and microsomal trout enzymes. Arch. Biochem. Biophys. 225 (1983) 55-65
47. Williams D.E., Okita R.T., Buhler D.R., and Masters B.S. Regiospecific hydroxylation of lauric acid at the (omega-1) position by hepatic and kidney microsomal cytochromes P-450 from rainbow trout. Arch. Biochem. Biophys. 231 (1984) 503-510