The comet assay: A sensitive method for detecting DNA damage in individual cells

Methods

Volumn 48, Issue 1, 2009, Pages 46-53

  Liao, Wenjuan ^a   McNutt, Michael A ^a   Zhu, Wei Guo ^a  

^a PEKING UNIVERSITY HEALTH SCIENCE CENTER   (China)

Author keywords

Comet assay; DNA damage and repair; DNA strand breaks; Single cell gel

Indexed keywords

CANCER RESEARCH; COMET ASSAY; DNA DAMAGE; DNA DETERMINATION; DNA STRAND BREAKAGE; GENETIC TOXICOLOGY; HUMAN; PRIORITY JOURNAL; REVIEW; TECHNIQUE;

APOPTOSIS; AZACITIDINE; CELL LINE, TUMOR; COMET ASSAY; DNA DAMAGE; DNA REPAIR; DOSE-RESPONSE RELATIONSHIP, DRUG; ETHIDIUM; FLUORESCENT DYES; HUMANS; HYDROGEN-ION CONCENTRATION; LUNG NEOPLASMS; MICROSCOPY, FLUORESCENCE; MUTAGENICITY TESTS; SENSITIVITY AND SPECIFICITY; SMALL CELL LUNG CARCINOMA;

EID: 65649138401     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2009.02.016     Document Type: Review

References (40)

1. Ostling O., and Johanson K.J. Biochem. Biophys. Res. Commun. 123 (1984) 291-298
2. Collins A.R. Mol. Biotechnol. 26 (2004) 249-261
3. Hoeijmakers J.H. Nature 411 (2001) 366-374
4. Olive P.L., and Durand R.E. Cytometry A 66 (2005) 1-8
5. Zhu W.G., Lakshmanan R.R., Beal M.D., and Otterson G.A. Cancer Res. 61 (2001) 1327-1333
6. Chai G., Li L., Zhou W., Wu L., Zhao Y., Wang D., Lu S., Yu Y., Wang H., McNutt M.A., Hu Y.G., Chen Y., Yang Y., Wu X., Otterson G.A., and Zhu W.G. PLoS ONE 3 (2008) e2445
7. Zhu W.G., Hileman T., Ke Y., Wang P., Lu S., Duan W., Dai Z., Tong T., Villalona-Calero M.A., Plass C., and Otterson G.A. J. Biol. Chem. 279 (2004) 15161-15166
8. Tice R.R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J.C., and Sasaki Y.F. Environ. Mol. Mutagen. 35 (2000) 206-221
9. Singh N.P., McCoy M.T., Tice R.R., and Schneider E.L. Exp. Cell Res. 175 (1988) 184-191
10. Olive P.L., Banath J.P., and Durand R.E. J. Natl. Cancer Inst. 82 (1990) 779-783
11. Collins A.R., Duthie S.J., and Dobson V.L. Carcinogenesis 14 (1993) 1733-1735
12. Anderson D., Yu T.W., and Browne M.A. Mutat. Res. 390 (1997) 69-77
13. Santos S.J., Singh N.P., and Natarajan A.T. Exp. Cell Res. 232 (1997) 407-411
14. Collins A.R., Dusinska M., Horvathova E., Munro E., Savio M., and Stetina R. Mutagenesis 16 (2001) 297-301
15. Singh N.P., Stephens R.E., and Schneider E.L. Int. J. Radiat. Biol. 66 (1994) 23-28
16. Olive P.L. Radiat. Res. 117 (1989) 79-92
17. Gedik C.M., Ewen S.W., and Collins A.R. Int. J. Radiat. Biol. 62 (1992) 313-320
18. Tebbs R.S., Cleaver J.E., Pedersen R.A., and Hartmann A. Mutagenesis 14 (1999) 437-438
19. Kizilian N., Wilkins R.C., Reinhardt P., Ferrarotto C., McLean J.R., and McNamee J.P. Biotechniques 27 (1999) 926-930
20. Olive P.L., Banath J.P., and Durand R.E. Radiat. Res. 122 (1990) 86-94
21. Collins A.R., Oscoz A.A., Brunborg G., Gaivao I., Giovannelli L., Kruszewski M., Smith C.C., and Stetina R. Mutagenesis 23 (2008) 143-151
22. Karpf A.R., Moore B.C., Ririe T.O., and Jones D.A. Mol. Pharmacol. 59 (2001) 751-757
23. Wang H., Zhao Y., Li L., McNutt M.A., Wu L., Lu S., Yu Y., Zhou W., Feng J., Chai G., Yang Y., and Zhu W.G. J. Biol. Chem. 283 (2008) 2564-2574
24. Rothkamm K., and Lobrich M. Proc. Natl. Acad. Sci. USA 100 (2003) 5057-5062
25. MacPhail S.H., Banath J.P., Yu Y., Chu E., and Olive P.L. Radiat. Res. 159 (2003) 759-767
26. Olive P.L., and Banath J.P. Exp. Cell Res. 221 (1995) 19-26
27. Henderson L., Wolfreys A., Fedyk J., Bourner C., and Windebank S. Mutagenesis 13 (1998) 89-94
28. Kiffe M., Christen P., and Arni P. Mutat. Res. 537 (2003) 151-168
29. Rundell M.S., Wagner E.D., and Plewa M.J. Environ. Mol. Mutagen. 42 (2003) 61-67
30. O'Callaghan Y.C., Woods J.A., and O'Brien N.M. Biochem. Pharmacol. 61 (2001) 1217-1226
31. Wilkins R.C., Kutzner B.C., Truong M., Sanchez-Dardon J., and McLean J.R. Cytometry 48 (2002) 14-19
32. Olive P.L., Vikse C.M., and Vanderbyl S. Radiother. Oncol. 50 (1999) 113-119
33. Gedik C.M., and Collins A. FASEB J. 19 (2005) 82-84
34. Pfuhler S., and Wolf H.U. Environ. Mol. Mutagen. 27 (1996) 196-201
35. Collins A.R., Mitchell D.L., Zunino A., de Wit J., and Busch D. Environ. Mol. Mutagen. 29 (1997) 152-160
36. McGlynn A.P., Wasson G., O'Connor J., McKerr G., McKelvey-Martin V.J., and Downes C.S. Cancer Res. 59 (1999) 5912-5916
37. Choucroun P., Gillet D., Dorange G., Sawicki B., and Dewitte J.D. Mutat. Res. 478 (2001) 89-96
38. Wang T.S., Chung C.H., Wang A.S., Bau D.T., Samikkannu T., Jan K.Y., Cheng Y.M., and Lee T.C. Chem. Res. Toxicol. 15 (2002) 1254-1258
39. Smith C.C., O'Donovan M.R., and Martin E.A. Mutagenesis 21 (2006) 185-190
40. Y. Yang, Y. Zhao, W. Liao, J. Yang, L. Wu, Z. Zheng, Y. Yu, W. Zhou, L. Li, J. Feng, H. Wang, W.G. Zhu, Neoplasia 11 (2009) 313-324.