Silver-stained comet assay for detection of apoptosis

BioTechniques

Volumn 27, Issue 5, 1999, Pages 926-930

  Kizilian, Narine ^a,b   Wilkins, R C ^a   Reinhardt, P ^a   Ferrarotto, C ^a   McLean, J R N ^a   McNamee, J P ^a  

^a HEALTH CANADA   (Canada)

^b HEALTH CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

APOPTOSIS; AQUEOUS SOLUTION; ARTICLE; CELL CULTURE; CELL VIABILITY; DNA DAMAGE; ELECTROPHORESIS; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MEASUREMENT; SILVER STAINING;

EID: 0032748896     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99275bm10     Document Type: Article

References (8)

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2. Merril, C.R. and M.E. Pratt 1986. A silver stain for the rapid quantitative detection of protein or nucleic acids on membranes or thin layer plates. Anal. Cell Res. 221:19-26.
3. Olive, P.L., G. Frazer and J.P. Banath. 1993. Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat. Res. 136:130-136.
4. Olive, P.L., D. Wlodek, R.E. Durand and J.P. Banath. 1992. Factors influencing DNA migration from individual cells subjected to gel electrophoresis.Exp. Cell Res. 198:259-267.
5. Ostling, O. and K.J. Johanson. 1984. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123:291-298.
6. Singh, N.P, M.T. McCoy, R.R. Tice and E.L. Schneider. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175:184-191.
7. Strauss, G.H.S. 1991. Non-random cell killing in cryopreservation: implications for performance of the battery of leukocyte tests (BLT), I. Toxic and immunotoxic effects. Mutat. Res. 252:1-15.
8. Wyllie, A.H, J.F.R. Kerr and A.R. Currie. 1980. Cell death: the significance of apoptosis. Int. Rev. Cytol. 65:251-306.