Large scale refolding and purification of the catalytic domain of human BACE-2 produced in E. coli

Protein and Peptide Letters

Volumn 16, Issue 2, 2009, Pages 121-131

  Emmons, T L ^a   Mildner, A M ^b   Lull, J M ^c   Leone, J W ^a   Fischer, H D ^a   Heinrikson, R L ^c   Tomasselli, A G ^a  

^a PFIZER INC   (United States)

^b Jardines de Los Soles II   (Argentina)

^c Proteos Inc   (United States)

Author keywords

Affinity column chromatography; BACE 2; Expression; Inclusion bodies; Protein refolding

Indexed keywords

ASPARTIC PROTEINASE; BACE2 PROTEIN, HUMAN; RECOMBINANT PROTEIN; SECRETASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CELL INCLUSION; CHEMISTRY; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENETICS; HUMAN; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PROTEIN FOLDING; SEQUENCE ANALYSIS; TEMPERATURE;

AMINO ACID SEQUENCE; AMYLOID PRECURSOR PROTEIN SECRETASES; ASPARTIC ENDOPEPTIDASES; CATALYTIC DOMAIN; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; INCLUSION BODIES; MOLECULAR SEQUENCE DATA; PROTEIN FOLDING; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; TEMPERATURE;

ESCHERICHIA COLI;

EID: 63449130335     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986609787316180     Document Type: Article

References (29)

1. Hardy, J.A. and Selkoe, D.J. The amyloid hypothesis of Alzheimer's disease: progress and problems on the road to therapeutics. (2002) Science, 297, 353-356.
2. Gandy, S. The role of cerebral amyloid β accumulation in common forms of Alzheimer disease. (2005) J. Clin. Invest., 115, 1121-1129.
3. Liu, K., Doms, R.W. and Lee, V.M. Glull Site Cleavage and N-Terminally Truncated Aβ Production upon BACE Overexpression. (2002) Biochemistry, 41, 3128-3136.
4. Iwata, N., Tsubuki, S., Takaki, Y., Shirotani, K., Lu, B., Gerard, N.P., Gerard, C., Hama, E., Lee, H.-J. and Saido, T.C. Metabolic regulation of brain Aβ by neprilysin. (2001) Science, 292, 1550-1552.
5. Kuroshkin, I.V. and Goto, S. Alzheimer's β-amyloid peptide specifically interacts with and is degraded by insulin degrading enzyme. (1994) FEBS Lett., 345, 33-37.
6. Allinson, T.M., Parkin, E.T., Turner, A.J. and Hooper, N.M. AD-AMs family members as amyloid precursor protein α-secretases. (2003) J. Neurosci. Res., 74, 342-352.
7. Fahrenholz, F. and Postina, R. α-Secretase activation - an approach to Alzheimer's disease therapy. (2006) Neurodegener. Dis., 3, 255-261.
8. Christensen, D.D. Changing the course of Alzheimer's disease: anti-amyloid disease-modifying treatments on the horizon. (2007) Prime Care Companion J. Clin. Psychiatry, 9, 32-41.
9. Takasugi, N., Tomita, T., Hayashi, I., Tsuruoka, M., Niimura, M., Takahashi, Y., Thinakaran, G. and Iwatsubo, T. The role of prese-nilin cofactors in the γ-secretase complex. (2003) Nature, 422, 438-441.
10. Kimberly, W.T., LaVoie, M.J., Ostaszewski, B.L., Ye, W., Wolfe, M.S. and Selkoe, D.J. γ-Secretase is a membrane protein complex comprised of presenilin, nicastrin, aph-1, and pen-2. (2003) Proc. Natl. Acad. Sci. USA, 100, 6382-6387.
11. Edbauer, D., Winkler, E., Regula, J.T., Pesold, B., Steiner, H. and Haass, C. Reconstitution of y-secretase activity. (2003) Nat. Cell Biol., 5, 486-488.
12. Yan, R., Bienkowski, M.J., Shuck, M.E., Miao, H., Tory, M.C., Pauley, A.M., Brashier, J.R., Stratman, N.C., Mathews, W.R., Buhl, A.E., Carter, D.B., Tomasselli, A.G., Parodi, L.A., Heinrik-son, R.L. and Gurney, M.E. Membrane-anchored aspartyl protease with Alzheimer's disease β-secretase activity. (1999) Nature, 402, 533-537.
13. Vassar, R., Bennett, B.D., Babu-Khan, S., Kahn, S., Mendiaz, E.A., Denis, P., Teplow, D.B., Ross, S., Amarante, P., Loeloff, R., Luo, Y., Fisher, S., Fuller, J., Edenson, S., Lile, J., Jarosinski, M.A., Biere, A.L., Curran, E., Burgess, T., Louis, J.C., Collins, F., Tre-anor, J., Rogers, G. and Citron, M. β-Secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. (1999) Science, 286, 735-741.
14. Sinha, S., Anderson, J.P., Barbour, R., Basi, G.S., Caccavello, R., Davis, D., Doan, M., Dovey, H.F., Frigon, N., Hong, J., Jacobson-Croak, K., Jewett, N., Keim, P., Knops, J., Lieberburg, I., Power, M., Tan, H., Tatsuno, G., Tung, J., Schenk, D., Seubert, P., Suomensaari, S.M., Wang, S., Walker, D. and John, V. Purification and cloning of amyloid precursor protein β-secretase from human brain. (1999) Nature, 402, 537-540.
15. Hussain, I., Powell, D., Howlett, D.R., Tew, D.G., Meek, T.D., Chapman, C., Gloger, I.S., Murphy, K.E., Southan, C.D., Ryan, D.M., Smith, T.S., Simmons, D.L., Walsh, F.S., Dingwall, C. and Christie, G. Identification of a novel aspartic protease (Asp 2) as β-secretase. (1999) Mol. Cell Neurosci., 14, 419-427.
16. Yan, R., Munzner, J.B., Shuck, M.E. and Bienkowski, M.J., BACE2 functions as an alternative α-secretase in cells. (2001) J. Biol. Chem., 276, 34019-34027.
17. Farzan, M., Schnitzler, C.E., Vasilieva, N., Leung, D. and Choe, H. BACE2, a β-secretase homolog, cleaves at the β site and within the amyloid- β region of the amyloid- β precursor protein. (2000) Proc. Natl. Acad. Sci. USA, 97, 9712-9717.
18. Basi, G., Frigon, N., Barbour, R., Doan, T., Gordon, G., McCon-logue, L., Sinha, S. and Zeller, M. Antagonistic effects of β-site amyloid precursor protein-cleaving enzymes 1 and 2 on β-amyloid peptide production in cells. (2003) J. Biol. Chem., 278, 31512-31520.
19. Hong, L., Koelsch, G., Lin, X., Wu, S., Terzyan, S., Ghosh, A.K., Zhang, X.C. and Tang, J. Structure of the protease domain of me-mapsin 2 (β - secretase) complexed with inhibitor. (2000) Science, 290, 150-153.
20. Patel, S., Vuillard, L., Cleasby, A., Murray, C.W. and Yon, J. Apo and inhibitor complex structures of BACE (β - secretase). (2004) J. Mol. Biol., 343, 407-416.
21. Stachel, S.J., Coburn, C.A, Steele, T.G., Jones, K.G., Loutzenhiser, E.F., Gregro, A.R., Rajapakse, H.A., Lai, M-T., Crouthamel, M-C., Xu, M., Tugusheva, K., Lineberger, J.E., Pietrak, B.L., Espeseth, A.S., Shi, X-P., Chen-Dodson, E., Holloway, M.K., Munshi, S., Simon, A.J., Kuo, L. and Vacca, J.P. Structure-based design of potent and selective cell-permeable inhibitors of human β-secretase (BACE-1). (2004) J. Med. Chem., 47, 6447-6450.
22. Ghosh, A.K., Bilcer, G., Hong, L., Koelsch, G. and Tang, J. Me-mapsin 2 (beta-secretase) inhibitor drug, between fantasy and reality. (2007) Curr. Alzheimer Res., 4, 418-422.
23. Ostermann, N., Eder, J., Eidhoff, U., Zink, F., Hassiepen, U., Wor-penberg, S., Maibaum, J., Simic, O., Hommel, U. and Gerhartz, B. Crystal structure of human BACE2 in complex with a hy-droxyethylamine transition-state inhibitor. (2006) J. Mol. Biol., 355, 249-261.
24. Tomasselli, A.G., Paddock, D.J., Emmons, T.L., Mildner, A.M., Leone, J.W., Lull, J.M., Cialdella, J.I., Prince, D.B., Fischer, H.D., Heinrikson, R.L. and Benson, T.E. High yield expression of human BACE constructs in Eschericia coli for refolding, purification, and high resolution diffracting crystal forms. (2008) Protein Pept. Lett., 15, 131-143.
25. Cole, S.R. and Vassar, R. The Alzheimer's disease beta-secretase enzyme, BACE1. (2007) Mol. Neurodegener., 2, 22-47.
26. nd ed), 1, 64-66.
27. Kim, Y.T., Downs, D., Wu, S., Dashti, A., Pan, Y., Zhai, P., Wang, X., Zhang, X.C. and Lin, X. Enzymic properties of recombinant BACE2. (2002) Eur. J. Biochem., 269, 5668-5677.
28. Lin, X., Koelsch, G., Wu, S., Downs, D., Dashti, A. and Tang, J. Human aspartic protease memapsin 2 cleaves the β-secretase site of β-amyloid precursor protein. (2000) Proc. Natl. Acad. Sci. USA, 97, 1456-1460.
29. Hussain, I., Christie, G., Schneider, K., Moore, S. and Dingwall, C. Prodomain processing of Aspl (BACE2) is autocatalytic. (2001) J. Biol. Chem., 276, 23322-328.