Taqman real-time PCR for the detection and enumeration of Saccharomyces cerevisiae in wine

Food Microbiology

Volumn 26, Issue 3, 2009, Pages 328-332

  Salinas, Francisco ^a   Garrido, Daniel ^a   Ganga, Angélica ^a   Veliz, Guido ^b   Martínez, Claudio ^a  

^a UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

^b Productos Roche Limitada ^*  (Chile)

Author keywords

Real time PCR; Refermentation; Saccharomyces cerevisiae

Indexed keywords

FUNGAL DNA;

ARTICLE; BACTERIAL COUNT; CHEMISTRY; DNA FRAGMENTATION; FERMENTATION; FOOD CONTROL; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; POLYMERASE CHAIN REACTION; QUALITY CONTROL; RANDOM AMPLIFIED POLYMORPHIC DNA; REPRODUCIBILITY; SACCHAROMYCES CEREVISIAE; SENSITIVITY AND SPECIFICITY; SPECIES DIFFERENCE; STANDARD; TIME; WINE;

COLONY COUNT, MICROBIAL; DNA FRAGMENTATION; DNA, FUNGAL; FERMENTATION; FOOD MICROBIOLOGY; POLYMERASE CHAIN REACTION; QUALITY CONTROL; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; REPRODUCIBILITY OF RESULTS; SACCHAROMYCES CEREVISIAE; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TIME FACTORS; WINE;

SACCHAROMYCES CEREVISIAE;

EID: 61649094947     PISSN: 07400020     EISSN: 10959998     Source Type: Journal    
DOI: 10.1016/j.fm.2008.12.001     Document Type: Article

References (20)

1. Bisson L. The biotechnology of wine yeast. Food Biotechnol. 18 (2004) 63-69
2. Besnard V., Federighi M., and Cappelier J.M. Development of a direct viable count procedure for the investigation of VBNC state in Listeria monocytogenes. Lett. Appl. Microbiol. 31 (2000) 77-81
3. Bleve G., Rizzotti L., Dellaglio F., and Torriani S. Development of reverse transcription (RT)-PCR and real-time RT-PCR assays for rapid detection and quantification of viable yeasts and molds contaminating yogurts and pasteurized food products. Appl. Environ. Microbiol. 69 (2003) 4116-4122
4. Del Mar Lleó M., Pierobon S., Tafi M.C., Signoretto C., and Canepari P. mRNA detection by reverse transcription-PCR for monitoring viability over time in an Enterococcus faecalis viable but nonculturable population maintained in a laboratory microcosm. Appl. Environ. Microbiol. 66 (2000) 4564-4567
5. Divol B., Miot-Sertier C., and Lonvaud-Funel A. Genetic characterization of strains of Saccharomyces cerevisiae responsible for 'refermentation' in Botrytis-affected wines. J. Appl. Microbiol. 100 (2006) 516-526
6. Divol B., and Lonvaud-Funel A. Evidence for viable but nonculturable yeasts in botrytis-affected wine. J. Appl. Microbiol. 99 (2005) 85-93
7. Fernandez-Espinar M., Barrio E., and Querol A. Analysis of the genetic variability in the species of the Saccharomyces sensu stricto complex. Yeast 20 (2003) 1213-1226
8. Ganga M.A., and Martínez C. Effect of wine yeast monoculture practice on the biodiversity of non-Saccharomyces yeasts. J. Appl. Microbiol. 96 (2004) 76-83
9. Hierro N., Esteve-Zarzoso B., Gonzalez A., Mas A., and Guillamon J.M. Real-time quantitative PCR (QPCR) and reverse transcription-QPCR for detection and enumeration of total yeasts in wine. Appl. Environ. Microbiol. 72 (2006) 7148-7155
10. Martorell P., Querol A., and Fernandez-Espinar M.T. Rapid identification and enumeration of Saccharomyces cerevisiae cells in wine by real-time PCR. Appl. Environ. Microbiol. 71 (2005) 6823-6830
11. McDougald D., Rice S.A., Weichart D., and Kjellberg S. Nonculturability: adaptation or debilitation. FEMS Microbiol. Ecol. 25 (1998) 1-9
12. Mills D.A., Johannsen E.A., and Cocolin L. Yeast diversity and persistence in botrytis-affected wine fermentations. Appl. Environ. Microbiol. 68 (2002) 4884-4893
13. Millet V., and Lonvaud-Funel A. The viable but non-culturable state of wine micro-organisms during storage. Lett. Appl. Microbiol. 30 (2000) 136-141
14. Mulè G., Susca A., Logrieco A., Stea G., and Visconti A. Development of a quantitative real-time PCR assay for the detection of Aspergillus carbonarius in grapes. Int. J. Food Microbiol. 111 (2006) S28-S34
15. Oliver J.D. The Public Health Significance of Viable But Nonculturable Bacteria. In: Colwell R.R., and Grimes D.J. (Eds). Nonculturable Microorganisms in the Environment (2000), American Society for Microbiology Press, Washington, DC 277-299
16. Phister T.G., and Mills D.A. Real-time PCR assay for detection and enumeration of Dekkera bruxellensis in wine. Appl. Environ. Microbiol. 69 (2003) 7430-7434
17. Pretorius I.S. Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking. Yeast 16 (2000) 675-729
18. Rasmussen R. Quantification on the LightCycler. In: Meuer S., Wittwer C., and Nakagawara K. (Eds). Rapid Cycle Real-time PCR, Method and Applications (2001), Springer Press, Heidelberg 21-34
19. Rawsthorne H., and Phister T.G. A real-time PCR assay for the enumeration and detection of Zygosaccharomyces bailii from wine and fruit juices. Int. J. Food Microbiol. 112 (2006) 1-7
20. Xufre A., Albergaria H., Inacio J., Spencer-Martins I., and Girio F. Application of fluorescence in situ hybridisation (FISH) to the analysis of yeast population dynamics in winery and laboratory grape must fermentations. Int. J. Food Microbiol. 108 (2006) 376-384