Kinesin's cover-neck bundle folds forward to generate force

Proceedings of the National Academy of Sciences of the United States of America

Volumn 105, Issue 49, 2008, Pages 19247-19252

  Khalil, Ahmad S ^a   Appleyard, David C ^a   Labno, Anna K ^b   Georges, Adrien ^a   Karplus, Martin ^c,d   Belcher, Angela M ^a   Hwang, Wonmuk ^e   Lang, Matthew J ^a  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c HARVARD UNIVERSITY   (United States)

^d UNIVERSITÉ LOUIS PASTEUR   (France)

^e Texas A and M University   (United States)

Author keywords

Biological motor; Force generation; Motor protein; Optical trap; Power stroke

Indexed keywords

KINESIN; MOLECULAR MOTOR;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DROSOPHILA MELANOGASTER; GENE MUTATION; KINETICS; MECHANOTRANSDUCTION; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; SIMULATION;

ANIMALS; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; KINESIN; MODELS, CHEMICAL; MUTAGENESIS; OPTICAL TWEEZERS; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY;

EID: 58049202168     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0805147105     Document Type: Article

References (31)

1. Vale RD (2003) The molecular motor toolbox for intracellular transport. Cell 112:467-480.
2. Kull FJ, Sablin EB, Lau R, Fletterick RJ, Vale RD (1996) Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature 380:550-555.
3. Vale RD, Milligan RA (2000) The way things move: Looking under the hood of molecular motor proteins. Science 288:88-95.
4. Geeves MA, Holmes KC (2005) The molecular mechanism of muscle contraction. Adv Protein Chem 71:161-193.
5. Rice S, et al. (1999)Astructural change in the kinesin motor protein that drives motility. Nature 402:778-784.
6. Case RB, Rice S, Hart CL, Ly B, Vale RD (2000) Role of the kinesin neck linker and catalytic core in microtubule-based motility. Curr Biol 10:157-160.
7. Rice S, et al. (2003) Thermodynamic properties of the kinesin neck-region docking to the catalytic core. Biophys J 84:1844-1854.
8. Hwang W, Lang MJ, Karplus M (2008) Force generation in kinesin hinges on cover-neck bundle formation. Structure 6:62-71.
9. Kikkawa M, et al. (2001) Switch-based mechanism of kinesin motors. Nature 411:439-445.
10. Sindelar CV, et al. (2002) Two conformations in the human kinesin power stroke defined by x-ray crystallography and EPR spectroscopy. Nat Struct Biol 9:844-848.
11. Block SM, Asbury CL, Shaevitz JW, Lang MJ (2003) Probing the kinesin reaction cycle with a 2D optical force clamp. Proc Natl Acad Sci USA 100:2351-2356.
12. Hwang W (2007) Calculation of conformation-dependent biomolecular forces. J Chem Phys 127:175104.
13. Block SM (2007) Kinesin motor mechanics: Binding, stepping, tracking, gating, and limping. Biophys J 92:2986-2995.
14. Berliner E, Young EC, Anderson K, Mahtani HK, Gelles J (1995) Failure of a single-headed kinesin to track parallel to microtubule protofilaments. Nature 373:718-721.
15. Khalil AS, et al. (2007) Single M13 bacteriophage tethering and stretching. Proc Natl Acad Sci USA 104:4892-4897.
16. Kojima H, Muto E, Higuchi H, Yanagida T (1997) Mechanics of single kinesin molecules measured by optical trapping nanometry. Biophys J 73:2012-2022.
17. Altman D, Sweeney HL, Spudich JA (2004) The mechanism of myosin VI translocation and its load-induced anchoring. Cell 116:737-749.
18. Wang MD, et al. (1998) Force and velocity measured for single molecules of RNA polymerase. Science 282:902-907.
19. Fisher ME, Kolomeisky AB (2001) Simple mechanochemistry describes the dynamics of kinesin molecules. Proc Natl Acad Sci USA 98:7748-7753.
20. Rosenfeld SS, Fordyce PM, Jefferson GM, King PH, Block SM (2003) Stepping and stretching: How kinesin uses internal strain to walk processively. J Biol Chem 278:18550-18556.
21. Mori T, Vale RD, Tomishige M (2007) How kinesin waits between steps. Nature 450:750-754.
22. Tomishige M, Stuurman N, Vale RD (2006) Single-molecule observations of neck linker conformational changes in the kinesin motor protein. Nat Struct Mol Biol 13:887-894.
23. Kikkawa M (2008) The role of microtubules in processive kinesin movement. Trends Cell Biol 18:128-135.
24. Sindelar CV, Downing KH (2007) The beginning of kinesin's force-generating cycle visualized at 9-Å resolution. J Cell Biol 177:377-385.
25. Endow SA, Waligora KW (1998) Determinants of kinesin motor polarity. Science 281:1200-1202.
26. Krishna MMG, Englander SW (2005) The N-terminal to C-terminal motif in protein folding and function. Proc Natl Acad Sci USA 102:1053-1058.
27. Mohanty S, Meinke JH, Zimmermann O, Hansmann UHE (2008) Simulation of Top7- CFr: A transient helix extension guides folding. Proc Natl Acad Sci USA 105:8004-8007.
28. Berliner E, et al. (1994) Microtubule movement by a biotinylated kinesin bound to streptavidin-coated surface. J Biol Chem 269:8610-8615.
29. Asbury CL, Fehr AN, Block SM (2003) Kinesin moves by an asymmetric hand-over- hand mechanism. Science 302:2130-2134.
30. Lang MJ, Asbury CL, Shaevitz JW, Block SM (2002) An automated two-dimensional optical force clamp for single molecule studies. Biophys J 83:491-501.
31. Svoboda K, Block SM (1994) Biological applications of optical forces. Annu Rev Biophys Biomol Struct 23:247-285.