How kinesin waits between steps

Nature

Volumn 450, Issue 7170, 2007, Pages 750-754

  Mori, Teppei ^a   Vale, Ronald D ^b   Tomishige, Michio ^a,b  

^a UNIVERSITY OF TOKYO   (Japan)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; KINESIN; MOLECULAR MOTOR; TUBULIN;

MOTILITY; PROTEIN;

ARTICLE; CONFORMATIONAL TRANSITION; FLUORESCENCE RESONANCE ENERGY TRANSFER; MICROTUBULE; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL;

EID: 36749068925     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature06346     Document Type: Article

References (28)

1. Vale, R. D. The molecular motor toolbox for intracellular transport. Cell 112, 467-480 (2003).
2. Hirokawa, N. & Takemura, R. Molecular motors and mechanisms of directional transport in neurons. Nature Rev. Neurosci. 6, 201-214 (2005).
3. Hackney, D. D. Evidence for alternating headcatalysis bykinesin during microtubule-stimulated ATP hydrolysis. Proc. Natl Acad. Sci. USA 91, 6865-6869 (1994).
4. Svoboda, K., Schmidt, C. F., Schnapp, B. J. & Block, S. M. Direct observation of kinesin stepping by optical trapping interferometry. Nature 365, 721-727 (1993).
5. Asbury, C. L., Fehr, A. N. & Block, S. M. Kinesin moves by an asymmetric hand-over-hand mechanism. Science 302, 2130-2134 (2003).
6. Kaseda, K., Higuchi, H. & Hirose, K. Alternate fast and slow stepping of a heterodimeric kinesin molecule. Nature Cell Biol. 5, 1079-1082 (2003).
7. Yildiz, A., Tomishige, M., Vale, R. D. & Selvin, P. R. Kinesin walks hand-over-hand. Science 303, 676-678 (2004).
8. Vale, R. D. & Milligan, R. A. The way things move: looking under the hood of molecular motor proteins. Science 288, 88-95 (2000).
9. Rice, S. et al. A structural change in the kinesin motor protein that drives motility. Nature 402, 778-784 (1999).
10. Skiniotis, G. et al. Nucleotide-induced conformations in the neck region of dimeric kinesin. EMBO J. 22, 1518-1528 (2003).
11. Asenjo, A. B., Weinberg, Y.&Sosa, H. Nucleotide binding and hydrolysis induces a disorder-order transition in the kinesin neck-linker region. Nature Struct. Mol. Biol. 13, 648-654 (2006).
12. Tomishige, M., Stuurman, N. & Vale, R. D. Single-molecule observations of neck linker conformational changes in the kinesin motor protein. Nature Struct. Mol. Biol. 13, 887-894 (2006).
13. Carter, N. J. & Cross, R. A. Kinesin's moonwalk. Curr. Opin. Cell Biol. 18, 61-67 (2006).
14. Valentine, M. T. & Gilbert, S. P. To step or not to step? How biochemistry and mechanics influence processivity in Kinesin and Eg5. Curr. Opin. Cell Biol. 19, 75-81 (2007).
15. Hackney, D. D. Processive motor movement. Science 316, 58-59 (2007).
16. Block, S. M. Kinesin motor mechanics: binding, stepping, tracking, gating, and limping. Biophys. J. 92, 2986-2995 (2007).
17. Kawaguchi, K. & Ishiwata, S. Nucleotide-dependent single- to double-headed binding of kinesin. Science 291, 667-669 (2001).
18. Uemura, S. et al. Kinesin-microtubule binding depends on both nucleotide state and loading direction. Proc. Natl Acad. Sci. USA 99, 5977-5981 (2002).
19. Carter, N. J. & Cross, R. A. Mechanics of the kinesin step. Nature 435, 307-312 (2005).
20. Auerbach, S. D. & Johnson, K. A. Alternating site ATPase pathway of rat conventional kinesin. J. Biol. Chem. 280, 37048-37060 (2005).
21. Alonso, M. C. et al. An ATP gate controls tubulin binding by the tethered head of kinesin-1. Science 316, 120-123 (2007).
22. Asenjo, A. B., Krohn, N.&Sosa, H. Configuration of the two kinesin motor domains during ATP hydrolysis. Nature Struct. Biol. 10, 836-842 (2003).
23. Hackney, D. D. The tethered motor domain of a kinesin-microtubule complex catalyzes reversible synthesis of bound ATP. Proc. Natl Acad. Sci. USA 102, 18338-18343 (2005).
24. Woehlke, G. et al. Microtubule interaction site of the kinesin motor. Cell 90, 207-216 (1997).
25. Kaseda, K., Higuchi, H. & Hirose, K. Coordination of kinesin's two heads studied with mutant heterodimers. Proc. Natl Acad. Sci. USA 99, 16058-16063 (2002).
26. Guydosh, N. R. & Block, S. M. Backsteps induced by nucleotide analogs suggest the front head of kinesin is gated by strain. Proc. Natl Acad. Sci. USA 103, 8054-8059 (2006).
27. Tomishige, M., Klopfenstein, D. R. & Vale, R. D. Conversion of Unc104/KIF1A kinesin into a processive motor after dimerization. Science 297, 2263-2267 (2002).
28. Sablin, E. P. & Fletterick, R. J. Coordination between motor domains in processive kinesins. J. Biol. Chem. 279, 15707-15710 (2004).