Mechanics of single kinesin molecules measured by optical trapping nanometry

Biophysical Journal

Volumn 73, Issue 4, 1997, Pages 2012-2022

  Kojima, Hiroaki ^a,d   Muto, Etsuko ^a   Higuchi, Hideo ^a   Yanagida, Toshio ^b,c  

^a JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c OSAKA UNIVERSITY MEDICAL SCHOOL   (Japan)

^d Ministry of Posts and Telecommunications   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; KINESIN; RHODAMINE;

ARTICLE; AXONEME; CATTLE; CHLAMYDOMONAS REINHARDTII; KINETICS; MICROTUBULE; MODEL; MUSCLE FORCE; NONHUMAN; OPTICS; VELOCITY;

BOS TAURUS; CHLAMYDOMONAS REINHARDTII;

EID: 0030824684     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(97)78231-6     Document Type: Article

References (25)

1. Block, S. M., and K. Svoboda. 1995. Analysis of high resolution recordings of motor movement. Biophys. J. 68:230s-241s.
2. Coppin, C. M., J. T. Finer, J. A. Spudich, and R. D. Vale. 1996. Detection of sub-8-nm movements of kinesin by high-resolution optical-trap microscopy. Proc. Natl. Acad. Sci. USA. 93:1913-1917.
3. Dupuis, D. E., W. H. Guilford, J. Wu, and D. M. Warshaw. 1997. Actin filament mechanics in the laser trap. J. Muscle Res. Cell Motil. 18: 17-30.
4. Finer, J. T., R. M. Simmons, and J. A. Spudich. 1994. Single myosin molecule mechanics: piconewton forces and nanometre steps. Nature. 368:113-119.
5. Funatsu, T., Y. Harada, M. Tokunaga, K. Saito, and T. Yanagida. 1995. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution. Nature. 374:555-559.
6. Harada, Y., K. Sakurada, T. Aoki, D. D. Thomas, and T. Yanagida. 1990. Mechanochemical coupling in actomyosin energy transduction studied by in vitro movement assay. J. Mol. Biol. 216:49-68.
7. Higuchi, H., E. Muto, Y. Inoue, and T. Yanagida. 1997. Kinetics of force generation by single kinesin molecules activated by laser photolysis of caged ATP. Proc. Natl. Acad. Sci. USA. 94:4395-4400.
8. Hirose, K., A. Lockhart, R. A. Cross, and L. A. Amos. 1996. Three-dimensional cryoelectron microscopy of dimeric kinesin and ncd motor domains on microtubules. Proc. Natl. Acad. Sci. USA. 93:9539-9544.
9. Howard, J., A. J. Hudspeth, and R. D. Vale. 1989. Movement of microtubules by single kinesin molecules. Nature. 342:154-158.
10. Huang, T-G., J. Suhan, and D. D. Hackney. 1994. Drosophila kinesin motor domain extending to amino acid position 392 is dimeric when expressed in Escherichia coli. J. Biol. Chem. 269:16502-16507.
11. Inoue, Y., Y. Y. Toyoshima, A. H. Iwane, S. Morimoto, H. Higuchi, and T. Yanagida. 1997. Movements of truncated kinesin fragments with a short or an artificial flexible neck. Proc. Natl. Acad. Sci. USA. 97: 7275-7280.
12. Ishijima, A., T. Doi, K. Sakurada, and T. Yanagida. 1991. Sub-piconewton force fluctuations of actomyosin in vitro. Nature. 352:301-306.
13. Ishijima, A., Y. Harada, H. Kojima, T. Funatsu, H. Higuchi, and T. Yanagida. 1994. Single-molecule analysis of the actomyosin motor using nano-manipulation. Biochem. Biophys. Res. Commun. 199: 1057-1063.
14. Ishijima, A., H. Kojima, H. Higuchi, Y. Harada, T. Funatsu, and T. Yanagida. 1996. Multiple- and single-molecule analysis of the actomyosin motor by nanometer-piconewton manipulation with a microneedle: unitary steps and forces. Biophys. J. 70:383-400.
15. Kagami, O., and R. Kamiya. 1992. Translocation and rotation of microtubules caused by multiple species of Chlamydomonas inner-arm dynein J. Cell. Sci. 103:653-664.
16. Kull, F. J., E. P. Sablin, R. Lau, R. J. Fretterick, and R. D. Vale. 1996. Crystal structure of the kinesin motor domain reveals a structural similarity to myosin. Nature. 380:550-555.
17. Kuznetsov, S. A., E. A. Vaisberg, N. A. Shanina, N. N. Magretova, V. Y. Chernyak, and V. I. Gelfand. 1988. The quaternary structure of bovine brain kinesin. EMBO J. 7:353-356.
18. Meyhoefer, E., and J. Howard. 1995. The force generated by a single kinesin molecule against an elastic load. Proc. Natl. Acad. Sci. USA. 92:574-578.
19. Song, Y., and E. Mandelkow. 1993. Recombinant kinesin motor domain binds to β-tubulin and decrates microtubules with a B surface lattice. Proc. Natl. Acad. Sci. USA. 90:1671-1675.
20. Svoboda, K., and S. M. Block. 1994a. Force and velocity measured for single kinesin molecules. Cell. 77:773-784.
21. Svoboda, K., and S. M. Block. 1994b. Biological applications of optical forces. Annu. Rev. Biophys. Biomol. Struct. 23:247-285.
22. Svoboda, K., C. F. Schmidt, B. J. Schnapp, and S. M. Block. 1993. Direct observation of kinesin stepping by optical trapping interferometry. Nature. 365:721-727.
23. Vale, R. D., T. Funatsu, D. W. Pierce, L. Romberg, Y. Harada, and T. Yanagida. 1996. Direct observation of single kinesin molecules moving along microtubules. Nature. 380:451-453.
24. Vale, R. D., T. S. Reese, and M. P. Sheets. 1985. Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Cell. 42:39-50.
25. Witman, G. B., J. Plummer, and G. Sander. 1978. Chlamydomonas flagellar mutants lacking radial spokes and central tubules. J. Cell Biol. 76:729-747.