An automated two-dimensional optical force clamp for single molecule studies

Biophysical Journal

Volumn 83, Issue 1, 2002, Pages 491-501

  Lang, Matthew J ^a   Asbury, Charles L ^a   Shaevitz, Joshua W ^a   Block, Steven M ^a,b  

^a STANFORD UNIVERSITY   (United States)

^b Mail Code 5020 ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

KINESIN; PROTEIN;

ARTICLE; AUTOMATION; CALIBRATION; CLAMP; COMPUTER; FORCE; MICROSCOPY; MICROTUBULE; MOLECULAR DYNAMICS; MOLECULE; MOTION; OPTICAL INSTRUMENTATION; PROTEIN ANALYSIS;

EID: 0036284131     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(02)75185-0     Document Type: Article

References (36)

1. Two-dimensional tracking of ncd motility by back focal plane interferometry
2. Criteria for noise and vibration in buildings and vehicles
3. Bead movement by single kinesin molecules studied with optical tweezers
4. Analysis of high resolution recordings of motor movement
5. Single myosin molecule mechanics: Piconewton forces and nanometre steps
6. Photonic force microscope based on optical tweezers and two-photon excitation for biological applications
7. Scanning-force microscope based on an optical trap
8. Interference model for back-focal-plane displacement detection in optical tweezers
9. Steps and fluctuations of Listeria monocytogenes during actin-based motility
10. Optical chopsticks: Digital synthesis of multiple optical traps
11. Movement and force produced by a single myosin head
12. Determining relative and axial displacement in optical traps
13. Characterization of photodamage to escherichia coli in optical traps
14. Sequence-dependent pauses observed for single lambda exonuclease molecules
15. Three-dimensional high-resolution particle tracking for optical tweezers by forward scattered light
16. Structure of the actin-myosin complex and its implications for muscle contraction
17. Three-dimensional structure of myosin subfragment-1: A molecular motor
18. A structural change in the kinesin motor protein that drives motility
19. Myosin-V stepping kinetics: A molecular model for processivity
20. Optical trapping of dielectric particles in arbitrary fields
21. X-ray structure of motor and neck domains from rat brain kinesin
22. Kinesin hydrolyses one ATP per 8-nm step
23. ADP-induced rocking of the kinesin motor domain revealed by single-molecule fluorescence polarization microscopy
24. Biological applications of optical forces
25. Force and velocity measured for single kinesin molecules
26. Direct observation of kinesin stepping by optical trapping interferometry
27. Single molecule imaging of fluorophores and enzymatic reactions achieved by objective-type total internal reflection fluorescence microscopy
28. The way things move: Looking under the hood of molecular motor proteins
29. The stiffness of rabbit skeletal actomyosin cross-bridges determined with an optical tweezers transducer
30. Versatile optical traps with feedback control
31. Construction of multiple-beam optical traps with nanometer-resolution position sensing
32. Single kinesin molecules studied with a molecular force clamp
33. Force and velocity measured for single molecules of RNA polymerase
34. The light chain binding domain of expressed smooth muscle heavy meromyosin acts as a mechanical lever
35. Parametric study of the forces on microspheres held by optical tweezers