Purification and characterization of keratinase from recombinant Pichia and Bacillus strains

Protein Expression and Purification

Volumn 64, Issue 1, 2009, Pages 24-31

  Radha, Selvaraj ^a   Gunasekaran, Paramasamy ^a  

^a Center for Excellence in Genomic Sciences   (India)

Author keywords

Affinity chromatography; Bacillus megaterium; Keratinase; Pichia pastoris; Purification

Indexed keywords

BACTERIAL PROTEIN; CATION; ENZYME INHIBITOR; HEAVY METAL; KERATINASE; PEPTIDE HYDROLASE; RECOMBINANT PROTEIN; SOLVENT;

ARTICLE; BACILLUS MEGATERIUM; BACTERIAL GENE; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; GLYCOSYLATION; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR WEIGHT; PH; PICHIA; PLASMID; SENSITIVITY AND SPECIFICITY; TEMPERATURE;

BACILLUS MEGATERIUM; BACTERIAL PROTEINS; CATIONS; CLONING, MOLECULAR; ENZYME INHIBITORS; ENZYME STABILITY; GENES, BACTERIAL; GLYCOSYLATION; HYDROGEN-ION CONCENTRATION; KINETICS; METALS, HEAVY; MOLECULAR WEIGHT; PEPTIDE HYDROLASES; PICHIA; PLASMIDS; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SOLVENTS; SUBSTRATE SPECIFICITY; TEMPERATURE;

BACILLUS LICHENIFORMIS; BACILLUS MEGATERIUM; PICHIA; PICHIA PASTORIS;

EID: 57649083803     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.10.008     Document Type: Article

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