Cloning and expression of keratinase gene in Bacillus megaterium and optimization of fermentation conditions for the production of keratinase by recombinant strain

Journal of Applied Microbiology

Volumn 103, Issue 4, 2007, Pages 1301-1310

  Radha, S ^a   Gunasekaran, P ^a  

^a MADURAI KAMARAJ UNIVERSITY   (India)

Author keywords

Bacillius megaterium; Bacillus licheniformis; Gene expression; Keratinase; Response surface methodology

Indexed keywords

BACTERIOLOGY; CLONING; ENZYME ACTIVITY; ESCHERICHIA COLI; FERMENTATION; POLYMERASE CHAIN REACTION; SURFACE PROPERTIES;

BACILLIUS MEGATERIUM; BACILLUS LICHENIFORMIS; BACILLUS MEGATERIUM; FERMENTATION CONDITIONS; GENES EXPRESSION; KERATINASE; MEGATERIUM; OPTIMISATIONS; RECOMBINANT STRAINS; RESPONSE-SURFACE METHODOLOGY;

GENE EXPRESSION;

BACTERIAL DNA; KERATIN; KERATINASE; XYLOSE;

BACTERIUM; CLONE; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; OPTIMIZATION; RECOMBINATION;

ARTICLE; BACILLUS LICHENIFORMIS; BACILLUS MEGATERIUM; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL STRAIN; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXTRACELLULAR SPACE; FERMENTATION OPTIMIZATION; GENE AMPLIFICATION; GENE EXPRESSION; GENE FUNCTION; GENE SEQUENCE; INOCULATION; INTRACELLULAR SPACE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROMOTER REGION; RECOMBINANT PLASMID; RESPONSE SURFACE METHOD; SHUTTLE VECTOR; WILD TYPE;

BACILLUS LICHENIFORMIS; BACILLUS MEGATERIUM; ESCHERICHIA COLI;

EID: 34848859511     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03372.x     Document Type: Article

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