A Bacillus megaterium plasmid system for the production, export, and one-step purification of affinity-tagged heterologous levansucrase from growth medium

Applied and Environmental Microbiology

Volumn 72, Issue 2, 2006, Pages 1677-1679

  Malten, Marco ^a   Biedendieck, Rebekka ^a   Gamer, Martin ^a   Drews, Ann Christin ^a   Stammen, Simon ^a   Buchholz, Klaus ^a   Dijkhuizen, Lubbert ^b   Jahn, Dieter ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

AFFINITY CHROMATOGRAPHY; ENZYMES; POLYPEPTIDES; PROTEINS; PURIFICATION;

AFFINITY-TAGGED HETEROLOGOUS LEVANSUCRASE; BACILLUS MEGATERIUM; BACILLUS MEGATERIUM PLASMID SYSTEM; MEGATERIUM ESTERASE LIPA SIGNAL PEPTIDE;

BACTERIA;

ESTERASE; LEVANSUCRASE; LIPA PROTEIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

BIOTECHNOLOGY;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACILLUS MEGATERIUM; CULTURE MEDIUM; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SYNTHESIS; NONHUMAN; PLASMID;

AFFINITY LABELS; BACILLUS MEGATERIUM; BACTERIAL PROTEINS; CHROMATOGRAPHY, AFFINITY; CULTURE MEDIA; GENE EXPRESSION; GENES, BACTERIAL; GENETIC VECTORS; HEXOSYLTRANSFERASES; LACTOBACILLUS REUTERI; PLASMIDS; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS;

BACILLUS MEGATERIUM; LACTOBACILLUS REUTERI;

EID: 33144482376     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.72.2.1677-1679.2006     Document Type: Article

References (7)

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