Similarities and specificities of fungal keratinolytic proteases: Comparison of keratinases of Paecilomyces marquandii and Doratomyces microsporus to some known proteases

Applied and Environmental Microbiology

Volumn 71, Issue 7, 2005, Pages 3420-3426

  Gradišar, Helena ^a   Friedrich, Jožica ^a   Križaj, Igor ^b   Jerala, Roman ^a  

^a NATIONAL INSTITUTE OF CHEMISTRY   (Slovenia)

^b JO EF STEFAN INSTITUTE   (Slovenia)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOCHEMISTRY; FUNGI; MICROPOROUS MATERIALS; PH EFFECTS; REACTION KINETICS; SKIN;

COLLAGENASE; ELASTASE; PROTEASES; TRYPSIN;

ENZYMES;

ALKALINE PROTEINASE; AROMATIC AMINO ACID; CHYMOTRYPSIN; COLLAGENASE; ELASTASE; FUNGAL ENZYME; KERATIN; KERATINASE; PROTEINASE; PROTEINASE K; SERINE PROTEINASE; SUBTILISIN; TRYPSIN;

ENZYME ACTIVITY; FUNGUS;

AMINO TERMINAL SEQUENCE; ARTICLE; DORATOMYCES MICROSPORUS; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; FUNGUS; HYDROLYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PAECILOMYCES; PAECILOMYCES MARQUANDII; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STRAIN DIFFERENCE; TEMPERATURE SENSITIVITY;

AMINO ACID SEQUENCE; ANIMALS; ASCOMYCOTA; CASEINS; CATTLE; HUMANS; HYDROGEN-ION CONCENTRATION; KERATINS; KINETICS; MOLECULAR SEQUENCE DATA; PAECILOMYCES; PEPTIDE HYDROLASES; SUBSTRATE SPECIFICITY; TEMPERATURE;

DORATOMYCES MICROSPORUS; FUNGI; PAECILOMYCES MARQUANDII;

EID: 22144488135     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.7.3420-3426.2005     Document Type: Article

References (39)

1. Beynon, R. J., and J. S. Bond. 1989. Proteolytic enzymes, a practical approach. Oxford University Press, Oxford, United Kingdom.
2. Böckle, B., B. Galunsky, and R. Müller. 1995. Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530. Appl. Environ. Microbiol. 61:3705-3710.
3. Bressollier, P., F. Letourneau, M. Urdaci, and B. Verneuil. 1999. Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus. Appl. Environ. Microbiol. 65:2570-2576.
4. Cheng, S. W., H. M. Hu, S. W. Shen, H. Takagi, M. Asano, and Y. C. Tsai. 1995. Production and characterization of keratinase of a feather-degrading Bacillus licheniformis PWD-1. Biosci. Biotechnol. Biochem. 59:2239-2243.
5. Dozie, I. N. S., C. N. Okeke, and N. C. Unaeze. 1994. A thermostable, alkaline-active, keratinolytic proteinase from Chrysosporium keratinophilum. World J. Microbiol. Biotechnol. 10:563-567.
6. Evans, K. L., J. Crowder, and E. S. Miller. 2000. Subtilisins of Bacillus spp. hydrolyze keratin and allow growth on feathers. Can. J. Microbiol. 46:1004-1011.
7. Farag, A. M., and M. A. Hassan. 2004. Purification, characterization and immobilization of a keratinase from Aspergilius oryzae. Enzyme Microb. Technol. 34:85-93.
8. Friedrich, A., and G. Antranikian. 1996. Keratin degradation by Fervidobacterium pennavorans, a novel thermophilic anaerobic species of the order Thermotogales. Appl. Environ. Microbiol. 62:2875-2882.
9. Friedrich, J., H. Gradišar, D. Mandin, and J. P. Chaumont. 1999. Screening fungi for synthesis of keratinolytic enzymes. Lett. Appl. Microbiol. 28:127-130.
10. Friedrich, J., and S. Kern. 2003. Hydrolysis of native proteins by keratinolytic protease of Doratomyces microsporus. J. Mol. Catal. B Enzymol. 21:35-37.
11. Fuchs, E. 1995. Keratins and the skin. Annu. Rev. Cell Dev. 11:123-153.
12. Gradišar, H., S. Kern, and J. Friedrich. 2000. Keratinase of Doratomyces microsporus. Appl. Microbiol. Biotechnol. 53:196-200.
13. Gregg, K., S. D. Wilson, D. A. D. Parry, and G. E. Rogers. 1984. A comparison of genomic coding sequences for feather and scale keratins: Structural and evolutionary implications. EMBO J. 3:175-178.
14. Han, X. Q., and S. Damodaran. 1998. Purification and characterization of Protease Q: a detergent- and urea-stable serine endopeptidase from Bacillus pumilus. J. Agric. Food Chem. 46:3596-3603.
15. Hublin, A., H. Gradišar, J. Friedrich, and D. Vasić- Raćki. 2002. Stability and stabilization of Doratomyces microsporus keratinase. Biocatal. Biotransform. 20:329-336.
16. Kunert, J. 1992. Effect of reducing agents on proteolytic and keratinolytic activity of enzymes of Microsporum gypseum. Mycoses 35:343-348.
17. Kunert, J., and E. Kasafirek. 1988. Preliminary characterization of extracellular proteolytic enzymes of dermatophytes by chromogenic substrates. J. Med. Vet. Mycol. 26:187-194.
18. Kunert, J., and Z. Stransky. 1988. Thiosulfate production from cystine by keratinolytic prokaryote Streptomyces fradiae. Arch. Microbiol. 150:600-601.
19. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
20. Lee, H., D. B. Suh, J. H. Hwang, and H. J. Suh. 2002. Characterization of a keratinolytic metalloprotease from Bacillus sp. SCB-3. Appl. Biochem. Biotechnol. 97:123-133.
21. Letourneau, F., V. Soussotte, P. Bressollier, P. Branland, and B. Verneuil. 1998. Keratinolytic activity of Streptomyces sp. S.K1-02: a new isolated strain. Lett. Appl. Microbiol. 26:77-80.
22. Lin, X., C. G. Lee, E. S. Casale, and J. C. H. Shih. 1992. Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl. Environ. Microbiol. 58:327-3275.
23. Lin, X., J. Tang, G. Koelsch, M. Monod, and S. Foundling. 1993. Recombinant Canditropsin, an extracellular aspartic protease from yeast Candida tropicalis. J. Biol. Chem. 268:20143-20147.
24. Lin, X., D. W. Kelemen, E. S. Miller, and J. C. H. Shih. 1995. Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD-1. Appl. Environ. Microbiol. 61:1469-1474.
25. Malviya, H. K., R. C. Rajak, and S. K. Hasija. 1992. Purification and partial characterization of two extracellular keratinases of Scopulariopsis brevicaulis. Mycopathologia 119:161-165.
26. Mignon, B., M. Swinnen, J. P. Bouchara, M. Hofinger, A. Nikkles, G. Pierard, C. Gerday, and B. Losson. 1998. Purification and characterization of a 31.5 kDa keratinolytic subtilizin-like serine protease from Microsporum canis and evidence of its secretion in naturally infected cats. Med. Mycol. 36:395-404.
27. Mukhopadhyay, R. P., and A. L. Chandra. 1990. Keratinase of a streptomycete. Indian J. Exp. Biol. 28:575-577.
28. Negi, M., R. Tsuboi, T. Matsui, and H. Ogawa. 1984. Isolation and characterization of proteinase from Candida albicans: substrate specificity. J. Investig. Dermatol. 83:32-36.
29. Onifade, A. A. 1998. A review: potentials for biotechnological applications of keratin-degrading microorganisms and their enzymes for nutritional improvement of feathers and other keratins as livestock feed resources. Bioresour. Technol. 66:1-11.
30. Qin, L. M., S. Dekio, and J. Jidoi. 1992. Some biochemical characteristics of a partially purified extracellular keratinase from Trichophyton schoenleinii. Zentralbl. Bakteriol. 277:236-244.
31. Riessen, S., and G. Antranikian. 2001. Isolation of Thermoanaerobacter keratinophilus sp. nov., a novel thermophilic, anaerobic bacterium with keratinolytic activity. Extremopbiles 5:399-408.
32. Rojanavanich, V., T. Yoshiike, R. Tsuboi, K. Takamori, and H. Ogawa. 1990. Purification and characterization of an extracellular proteinase from Hendersonula toruloidea. Infect. Immun. 58:2856-2861.
33. Santos, R. M. D. B., A. A. P. Firmino, C.M. de Sá, and C. R. Felix. 1996. Keratinolytic activity of Aspergillus fumigatus fresenius. Curr. Microbiol. 33:364-370.
34. Suh, H. J., and H. K. Lee. 2001. Characterization of a keratinolytic serine protease from Bacillus subtilis KS-1. J. Protein Chem. 20:165-169.
35. Suntornsuk, W., and L. Suntornsuk. 2003. Feather degradation by Bacillus sp. FK 46 in submerged cultivation. Bioresour. Technol. 86:293-243.
36. Takami, H., F. Nakamura, R. Aono, and K. Hirokoshi. 1992. Degradation of human hair by a thermostable alkaline proteinase from Bacillus sp. no. AH 101. Biosci. Biotechnol. Biochem. 56:1667-1669.
37. Takiuchi, I., Y. Sei, H. Takagi, and M. Negi. 1984. Partial characterization of the extracellular keratinase from Microsporum canis. Sabouraudia: J. Med. Vet. Mycol. 22:219-224.
38. Tsuboi, R., I. J. Ko, K. Takamori, and H. Ogawa. 1989. Isolation of a keratinolytic proteinase from Trichophyton mentagrophytes with enzymatic activity at acidic pH. Infect. Immun. 57:3479-3483.
39. Yamamura, S., Y. Morita, Q. Hasan, K. Yokoyama, and E. Tamiya. 2002. Keratin degradation. A cooperative action of two enzymes from Stenotrophomonas sp. Biochem. Biophys. Res. Commun. 294:1138-1143.