Purification and some properties of a keratinolytic enzyme from an alkaliphilic nocardiopsis sp. TOA-1

Bioscience, Biotechnology and Biochemistry

Volumn 66, Issue 1, 2002, Pages 164-167

  Mitsuiki, Shinji ^a,c   Sakai, Masashi ^a   Moriyama, Yasushi ^b   Goto, Masatoshi ^c   Furukawa, Kensuke ^c  

^a KYUSHU SANGYO UNIVERSITY   (Japan)

^b TOTO LTD   (Japan)

^c KYUSHU UNIVERSITY   (Japan)

Author keywords

Alkaline protease; Alkaliphilic actinomycetes; Keratinolytic enzyme; Nocardiopsis sp

Indexed keywords

ACTINOBACTERIA; ACTINOBACTERIA (CLASS); NOCARDIOPSIS; NOCARDIOPSIS SP. TOA-1;

4 CHLOROMERCURIBENZOIC ACID; BACTERIAL DNA; BENZYLSULFONYL FLUORIDE; CASEIN; DODECYL SULFATE SODIUM; DYFLOS; EDETIC ACID; KERATIN; PROTEINASE INHIBITOR; SERINE PROTEINASE;

ACTINOMYCETALES; ARTICLE; CLASSIFICATION; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

ACTINOMYCETALES; BASE SEQUENCE; CASEINS; DNA, BACTERIAL; EDETIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; ISOFLUROPHATE; KERATINS; MOLECULAR SEQUENCE DATA; P-CHLOROMERCURIBENZOIC ACID; PHENYLMETHYLSULFONYL FLUORIDE; PROTEASE INHIBITORS; SERINE ENDOPEPTIDASES; SODIUM DODECYL SULFATE; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 0036370885     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.66.164     Document Type: Article

References (13)

1. Moriyama, Y., Nawata, N., Tsuda, T., and Nitta, M., Occurrence of Moulds in Japanese Bathroom. Int. Biodeter. Biodegr., 30, 47-55 (1992).
2. Kieser, T., Bibb, M. J., Butter, B. J., Chater, K. F., and Hoopwood, D. A., Practical Streptomyces genetics. John Innes Centre, Nowich, UK, pp.161-171 (2000).
3. Mehling, A., Wehmeier, U. F., and Piepersberg, W., Nucleotide sequence of Streptomycete 16S ribosomal DNA: towards a specific identification system for streptomycetes using PCR. Microbiology, 141, 2139-2147 (1995).
4. Tsujibo, H., Miyamoto, K., Hasegawa, T., and Inamori, Y., Purification and characterization of two types of alkaline serine proteases produced by an alkalophilic actinomycete. J. Appl. Bacterol., 69, 520-529 (1990).
5. Nakai, R., Horinouchi, S., and Beppu, T., Cloning and nucleotide sequence of a cellulase gene, casaA, from an alkalophilic Streptomyces strain. Gene, 65, 229-238 (1988).
6. Moriyama, Y., Matsushita, H., and Kawae, Y., Some degradative enzymatic activities associated with fungi isolated from bathroom. J. Antibact. Antifung. Agents., 24, 407-413 (1996).
7. Takami, H., Akiba, T., and Horikoshi, K., Characterization of an alkaline protease from Bacillus sp. AH-101. Appl. Microbiol. Biotechnol., 33, 519-523 (1990).
8. Takami, H., Akiba, T., and Horikoshi, K., Production of extremely thermostable alkaline protease from Bacillus sp. AH-101. Appl. Microbiol. Biotechnol., 30, 120-124 (1989).
9. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., Protein mesurement with the Folin phenol regent. J. Biol. Chem., 193, 265-275 (1951).
10. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).
11. Henderson, G., Krygsman, P., Liu, C.-J., Davey, C. C., and Maker, L. T., Characterization and Structure of genes for protease A and B from Streptomyces griseus. J. Bacteriol., 169, 3778-3784 (1987).
12. Kitadokoro, K., Tsuzuki, H., Nakamura, E., Sato, T., and Teraoka, H., Purification, characterization, primary structure, crystallization and preliminary crystallographic study of a serine protease from Streptomyces fradiae ATCC 14544. Eur. J. Biochem., 220, 55-61 (1994).
13. Takami, H., Nogi, Y., and Horikoshi, K., Reidentification of keratinase-producing facultatively alkaliphilic Bacillus sp. AH-101 as Bacillus halodu-rans. Extremophiles., 3, 293-296 (1999).