Pattern technologies for structuring neuronal networks on MEAs

Advances in Network Electrophysiology: Using Multi-Electrode Arrays

Volumn , Issue , 2006, Pages 153-189

  Chang, John C ^a   Wheeler, Bruce C ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 57349144456     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1007/0-387-25858-2_7     Document Type: Chapter

References (131)

1. Allara, D. L., Parikh, A. N., and Rondelez, F. (1995). Evidence for a unique chain organization in long chain silane monolayers deposted on two widely different solid substrates. Langmuir 11:2357-2360.
2. Allen, G. C., Tucker, P. M., Capon, A., and Parsons, R. (1974). X-ray photoelectron spectroscopy of adsorbed oxygen and carbonaceous species on platinum electrodes. J. Electroanal. Chem. 50:335-343.
3. Anderson, J. R., Chiu, D. T., Jackman, R. J., Cherniavskaya, O., McDonald, J. C., Wu, H., Whitesides, S. H., and Whitesides, G. M. (2000). Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping. Anal. Chem. 72:3158-3164.
4. Augustynski, J., Koudelka, M., and Sanchez, J. (1984). ESCA study of the state of iridium and oxygen in electrochemically and thermally formed iridium oxide films. J. Electroanal. Chem. 160:233-248.
5. Banker, G., and Goslin, K. (eds.). (1998). Culturing Nerve Cells. MIT Press, Cambridge.
6. Bekos, E. J., Ranieri, J. P., Aebischer, P., Gardella, J. A., Jr., and Bright, F. V. (1995). Structural changes of bovine serum albumin upon adsorption to modified fluoropolymer substrates used for neural cell attachment studies. Langmuir 11:984-989.
7. Bernard, A., Delamarche, E., Schmid, H., Michel, B., Bosshard, H. R., and Biebuyck, H. (1998). Printing patterns of proteins. Langmuir 14:2225-2229.
8. Bernard, A., Fitzli, D., Sonderegger, P., Delamarche, E., Michel, B., Bosshard, H. R., and Biebuyck, H. (2001). Affinity capture of proteins from solution and their dissociation by contact printing. Nat. Biotech. 19:866-869.
9. Bhatia, S. K., Shriver-Lake, L. C., Prior, K. J., Georger, J. H., Calvert, J. M., Bredehorst, R., and Ligler, F. S. (1989). Use of Thiol-Terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces. Anal. Biochem. 178:408-413.
10. Boppart, S. A., Wheeler, B. C., and Wallace, C. S. (1992). A flexible perforated microelectrode array for extended neural recordings. IEEE Trans. Biomed. Eng. 39:37-42.
11. Branch, D. (2000). Elastomeric stamping: Design considerations for long-term maintenance of neuronal networks. Doctoral Dissertation. Department of Biophysics and Computational Biology. University of Illinois, Urbana, IL.
12. Branch, D. W., Corey, J. M., Weyhenmeyer, J. A., Brewer, G. J., and Wheeler, B. C. (1998). Microstamp patterns of biomolecules for high-resolution neuronal networks. Med. Biol. Eng. Comp. 36:135-141.
13. Branch, D. W., Wheeler, B. C., Brewer, G. J., and Leckband, D. E. (2000). Long-term maintenance of patterns of hippocampal pyramidal cells on substrates of polyethylene glycol and microstamped polylysine. IEEE Trans. Biomed. Eng. 47:290-300.
14. Branch, D. W., Wheeler, B. C., Brewer, G. J., and Leckband, D. E. (2001). Long-term stability of grafted polyethylene glycol surfaces for use with microstamped substrates in neuronal cell culture. Biomaterials 22:1035-1047.
15. Brummer, S. B., McHardy, J., and Turner, M. J. (1977). Electrical stimulation with pt electrodes: Trace analysis for dissolved platinum and other dissolved electrochemical products. Brain Behav. Evol. 14:10-22.
16. Carmena, J. M., Lebedev, M. A., Crist, R. E., O'Doherty, J. E., Santucci, D. M., Dimitrov, D., Patil, P. G., Henriquez, C. S., and Nicolelis, M. A. (2003). Learning to control a brainmachine interface for reaching and grasping by primates. PLos Biol. 1:E42.
17. Caroni, P. (1998). Driving the growth cone. Science 281:1465-1466.
18. Cash, S., Zucker, R. S., and Poo, M.-M. (1996). Spread of synaptic depression mediated by presynaptic cytoplasmic signaling. Science 272:998-1001.
19. Chang, J. (2002). Technologies for and electrophysiological studies of structured, living, neuronal networks on microelectrode arrays. Doctoral Dissertation. Department of Electrical Engineering. University of Illinois, Urbana.
20. Chang, J., Brewer, G. J., and Wheeler, B. C. (2000). Microelectrode array recordings of patterned hippocampal neurons for four weeks. Biomed. Microdev. 2:245-253.
21. Chang, J. C., Brewer, G. J., and Wheeler, B. C. (2001a). Modulation of neural network activity by patterning. Biosens. Bioelectron. 17:527-533.
22. Chang, J. C., Brewer, G. J., and Wheeler, B. C. (2001b). Spatial patterningofneuronal cultures increases apparent network activity. 31st Annual Meeting. Society for Neuroscience, San Diego, CA.
23. Chang, J. C., Brewer, G. J., and Wheeler, B. C. (2003a). A modified microstamping technique enhances polylysine transfer and neuronal cell patterning. Biomaterials 24:2863-2870.
24. Chang, J. C., Brewer, G. J., and Wheeler, B. C. (2003b). A patterned substrate modulates glial development and synaptic density in a serum-free hippocampal culture. 33rd Annual Meeting. Society for Neuroscience, New Orleans.
25. Chaudhury, M. (1995). Self-assembled monolayers on polymer surfaces. Biosens. Bioelectron. 10:785-788.
26. Cheng, S.-S., Chittur, K. K., Sukenik, C. N., Culp, L. A., and Lewandowska, K. (1994). The conformation of fibronectin on self-assembled monolayers with different surface composition: An FTIR/ATR study. J. Coll. Int. Sci. 162:135-143.
27. Chiu, D. T., Jeon, N. L., Huang, S., Kane, R. S., Wargo, C. H., Choi, I. S., Ingber, D. E., and Whitesides, G. M. (2000). Patterned deposition of cells and proteins onto surfaces by using three-dimentional microfluidic systems. Proc. Nat. Acad. Sci. 97:2408-2413.
28. Chou, N. J., Paraszczak, J., Babich, E., Heidenreich, J., Chaug, Y. S., and Goldblatt, R. D. (1987). X-ray photoelectron and infrared spectroscopy of microwave plasma etched polyimide surfaces. J. Vac. Sci. Technol. A 5:1321-1326.
29. Clark, E. A. and Brugge, J. S. (1995). Integrins and signal transduction pathways: The road taken. Science 268:233-239.
30. Corey, J. M. (1997). Cell patterning: Building living neural networks. Doctoral Dissertation, Neuroscience Program. University of Illinois, Urbana.
31. Corey, J. M. and Feldman, E. L. (2003). Substrate patterning: An emerging technology for the study of neuronal behavior. Exp. Neurol. 184:S89-S96.
32. Corey, J. M., Wheeler, B. C., and Brewer, G. J. (1991). Compliance of hippocampal neurons to patterned substrate networks. J. Neurosci. Res. 30:300-307.
33. Corey, J. M., Wheeler, B. C., and Brewer, G. J. (1996). Micrometer resolution silane-based patterning of hippocampal neurons: Critical variables in photoresist ad laser ablation processes for substrate fabrication. IEEE Trans. Biomed. Eng. 43:944-955.
34. Cyster, L. A., Grant, D. M., Parker, K. G., and Parker, T. L. (2002). The effect of surface chemistry and structure of titanium nitride (TiN) films on primary hippocampal cells. Biomol. Eng. 19:171-175.
35. Dubois, L. and Nuzzo, R. (1992). Synthesis, structure, and properties of model organic surfaces. Annu. Rev. Phys. Chem. 43:437-463.
36. Dunn, D. S., Grant, J. L., and McClure, D. J. (1989). Texturing of polyimide films during O2/CF4 sputter etching. J. Vac. Sci. Technol. A 7:712-1718.
37. Egert, U., Schlosshauer, B., Fennrich, S., Nisch, W., Fejtl, M., Knott, T., Mueller, T., and Haemmerle, H. (1998). A novel organotypic long-term culture of the rat hippocampus on substrate-integrated multielectrode arrays. Brain Res. Prot. 2:229-242.
38. Engert, F. and Bonhoeffer, T. (1997). Synapse specificity of long-term potentiation breaks down at short distances. Nature 388:279-284.
39. Esch, T., Lemmon, V., and Banker, G. (1999). Local presentation of substrate molecules directs axon specification by cultured hippocampal neurons. J. Neurosci. 19:6417-6426.
40. Facci, J. and Murray, R. W. (1980). Silanization and non-aqueous electrochemistry of two oxide states on platinum electrodes. J. Electroanal. Chem. 112:221-229.
41. Ferguson, G. S., Chaudhury, M. K., Biebuyck, H. A., and Whitesides, G. M. (1993). Monolayers on disordered substrates: Self-assembly of alkyltrichlorosilanes on surface-modified polyethylene and poly (dimethylsiloxane). Macromolecules 26:5870-5875.
42. Firestone, M. A., Shank, M. L., Sligar, S. G., and Bohn, P. W. (1996). Film architecture in biomolecular assemblies. Effect of linker on the orientation of genetically engineered surface-bound proteins. J. Am. Chem. Soc. 118:9033-9041.
43. Freed, C. R., Greene, P. E., Breeze, R. E., Tsai, W.-Y., Du Mouchel, W., Kao, R., Dillon, S., Winfield, H., Culver, S., Trojanowski, J. Q., Eidelberg, D., and Fahn, S. (2001). Transplantation of embryonic dopamine neurons for severe Parkinson's disease. N. Engl. J. Med. 344:710-719.
44. Fromherz, P. and Schaden, H. (1994). Defined neuronal arborizations by guided outgrowth of leech neurons in culture. Eur. J. Neurosci. 6:1500-1504.
45. Gaehwiler, B. H., Thompson, S. M., McKinney, R. A., Debanne, D., and Robertson, R. T. (1998). Organotypic slice cultures of neural tissue. In: Banker, G. and Goslin, K., eds., Culturing Nerve Cells. The MIT Press, Cambridge.
46. Garbassi, F., Morra, M., and Occhiello, E. (1998). Polymer Surfaces: From Physics to Technology. John Wiley & Sons, New York.
47. Gesteland, R. C., Howland, B., Lettvin, J. Y., and Pitts, W. H. (1959). Comments on microelectrodes. Proc. IRE 47:1856-1862.
48. Gross, G. W. (1979). Simultaneous single unit recording in vitro with a photoetched laser deinsulated gold multimicroelectrode surface. IEEE Trans. Biomed. Eng. 26:273-279.
49. Gross, G. W. (1994). Internal dynamics of randomized mammalian neuronal networks in culture. In: Stenger, D. A. and McKenna, T. M., eds., Enabling Technologies for Cultured Neural Networks. Academic Press, San Diego, pp. 277-317.
50. Gross, G. W., Harsch, A., Rhoades, B. K., and Gopel, W. (1997). Odor, drug and toxinanalysis with neuronal networks in vitro: Extracellular array recording of network responses. Biosens. Bioelectron. 12:373-393.
51. Hammond, J. S. and Winograd, N. (1977). XPS spectroscopic study of potentiostatic and galvanostatic oxidation of Pt electrodes in H2SO4 and HClO4. J. Electroanal. Chem. 78:55-69.
52. Hari, A., Djohar, B., Skutella, T., and Montazeri, S. (2004). Neurotrophins and extracellular matrix molecules modulate sensory axon outgrowth. Int. J. Devl. Neurosci. 22:113-117.
53. Hermanson, G. T. (1996). Bioconjugate Techniques. Academic Press, Inc., San Diego.
54. Hickman, J. J. and Stenger, D. A. (1994). Interactions of cultured neurons with defined surfaces. In: Stenger, D. A. and McKenna, T. M., eds., Enabling Technologies for Cultured Neural Networks. Academic Press, San Diego, pp. 51-76.
55. Hoepker, V., Shewan, D., Tessier-Lavigne, M., Poo, M.-M., and Holt, C. (1999). Growthcone attraction to netrin-1 is converted to repulsion by laminin-1. Nature 401:69-73.
56. Hoffman, A. S. (1996). Surface modification of polymers: Physical, chemical, mechanical and biological methods. Macromolec. Symp. 101:443-454.
57. Hook, D. J., Vargo, T. G., Gardella, J. A., Jr., Litwiler, K. S., and Bright, F. V. (1991). Silanization of radio frequency glow discharge modified expanded poly (tetrafluoroethylene) using (aminopropyl) triethoxysilane. Langmuir 7:142-151.
58. Ingber, D. E. (1997). Tensegrity: The architectural basis of cellular mechanotransduction. Ann. Rev. Physiol. 59:575-599.
59. Israelachvili, J. (1997). The different faces of poly (ethylene glycol). Proc. Nat. Acad. Sci. 94:8378-8379.
60. James, C. D., Davis, R., Meyer, M., Turner, A., Turner, S., Withers, G., Kam, L., Banker, G., Craighead, H., Isaacson, M., Turner, J., and Shain, W. (2000). Aligned microcontact printing of micrometer-scale poly-L-lysine for controlled growth of cultured neurons on planar microelectrode arrays. IEEE Trans. Biomed. Eng. 47:17-21.
61. Jimbo, Y., Kawana, A., Parodi, P., and Torre, V. (2000). The dynamics of a neuronal culture of dissociated cortical neurons of neonatal rats. Biol. Cybern. 83:1-20.
62. Jimbo, Y., Robinson, H. P. C., and Kawana, A. (1993). Simultaneous measurement of intracellular calcium and electrical activity from patterned neural networks in culture. IEEE Trans. Biomed. Eng. 40:804-810.
63. Jimbo, Y., Robinson, H. P. C., and Kawana, A. (1998). Strengthening of synchronized activity by tetanic stimulation in cortical cultures: Application of planar electrode arrays. IEEE Trans. Biomed. Eng. 45:1297-1304.
64. Jimbo, Y., Tateno, T., and Robinson, H. P. C. (1999). Simultaneous induction of pathwayspecific potentiation and depression in networks of cortical neurons. Biophys. J. 76:670-678.
65. Jo, B.-H., Van Lerberghe, L. M, Motsegood, K. M., and Beebe, D. J. (2000). Three-dimensional micro-channel fabrication in polydimethylsiloxane (PDMS) elastomer. J. Microelectromech. Syst. 9:76-81.
66. Jones, L. L., Sajed, D., and Tuszynski, MH. (2003). Axonal regeneration through regions of chondroitin sulfate proteoglycan deposition after spinal cord injury: A balance of permissiveness and inhibition. J. Neurosci. 23:9276-9288.
67. Jung, D. R., Cuttino, D. S., Pancrazio, J. J., Manos, P., Cluster, T., Sathanoori, R. S., Aloi, L. E., Coulombe, M. G., Czarnaski, M. A., Borkholder, D. A, Kovacs, G. T. A., Bey, P., Stenger, D. A., and Hickman, J. J. (1998). Cell-based sensor microelectrode array characterized by imaging x-ray photoelectron spectroscopy, scanning electron microscopy, impedance measurements, and extracellular recordings. J. Vac. Sci. Technol. A 16:1183-1188.
68. Kandel, E. R., Schwartz, J. H., and Jessell, T. M. (eds.) (2000). Principles of Neural Science. McGraw-Hill, New York.
69. Kane, R. S., Takayama, S., Ostuni, E., Ingber, D. E., and Whitesides, G. M. (1999). Patterning proteins and cells using soft lithography. Biomaterials 20:2363-2376.
70. Kleinfeld, D., Kahler, K. H., and Hockberger, P. E. (1988). Controlled outgrowth of dissociated neurons on patterned substrates. J. Neurosci. 8:4098-4120.
71. Kumar, A., Biebuyck, H. A., and Whitesides, G. M. (1994). Patterning self-assembled monolayers: Applications in materials science. Langmuir 10:1498-1511.
72. Laibinis, P. E., Hickman, J. J., Wrighton, M. S., and Whitesides, G. M. (1989). Orthogonal self-assembled monolayers: Alkanethiols on gold and alkane carboxylic acids on alumina. Science 245:845-847.
73. Larson, G. (1991). An introduction to organosilicon chemistry, Hul's Silicon Compounds-Register And Review (1991), pp. 8-23.
74. Lauer, L., Vogt, A., Yeung, C. K., Knoll, W., and Offenhaeusser, A. (2002). Electrophysiological recordings of patterned rat brain stem slice neurons. Biomaterials 23:3123-3130.
75. Lee, K.-W. and Viehbeck, A. (1994). Wet-process surface modification of dielectric polymers: adhesion enhancement and metallization. IBM J. Res. Dev. 38:457-474.
76. Lenhard, J. and Murray, R. (1977). Chemically modified electrodes part vii. Covalent bonding of a reversible electrode reactant to pt electrodes using an organosilane reagent. J. Electroanal. Chem. 78:195-201.
77. Letourneau, P. C. (1975). Cell-to-substratum adhesion and guidance of axonal elongation. Dev. Biol. 44:92-101.
78. Liu, Q.-Y., Coulombe, M., Dumm, J., Shaffer, K. M., Schaffner, A. E., Barker, J. L., Pancrazio, J. J., Stenger, D. A., and Ma, W. (2000). Synaptic connectivity in hippocampal neuronal networks cultured on micropatterned surfaces. Dev. Brain Res. 120:223-231.
79. Lochter, A., Taylor, J., Braunewell, K.-H., Holm, J., and Schachner, M. (1995). Control of neuronal morphology in vitro: Interplay between adhesive substrate forces and molecular instruction. J. Neurosci. Res. 42:145-158.
80. Lom, B., Healy, K. E., and Hockberger, P. E. (1993). A versatile technique for patterning biomolecules onto glass coverslips. J. Neurosci. Meth. 50:385-397.
81. Lopez-Garcia, J. C., Arancio, O., Kandel, E. R., and Baranes, D. (1996). A presynaptic locus for long-term potentiation of elementary synaptic transmission at mossy fiber synapse in culture. Proc. Nat. Acad. Sci. 93:4712-4717.
82. Ma, W., Liu, Q.-Y., Jung, D., Manos, P., Pancrazio, J. J., Schaffner, A. E., Barker, J. L., and Stenger, D. A. (1998). Central neuronal synapse formation on micropatterned surfaces. Dev. Brain Res. 111:231-243.
83. McDonald, J. C., Duffy, D. C., Anderson, J R., Chiu, D. T., Wu, H., Schueller, O. J. A., and Whitesides, G. M. (2000). Fabrication of microfluidic systems in poly (dimethylsiloxane). Electrophoresis 21:27-40.
84. Ming, G.-I., Wong, S. T., Henley, J., Yuan, X.-B., Song, H.-J., Spitzer, N. C., and Poo, M.-M. (2002). Adaptation in the chemotactic guidance of nerve growth cones. Nature 417:411-418.
85. Morefield, S. I., Keefer, E. W., Chapman, K. D., and Gross, G. W. (2000). Drug evaluations using neuronal networks cultured on microelectrode arrays. Biosens. Bioelectron. 15:383-396.
86. Moses, P. R., Wier, L. M., Lennox, J. C., Finklea, H. O., Lenhard, J. R., and Murray, R. W. (1978). X-ray photoelectron spectroscopy of alkylamine-silanes bound to metal oxide electrodes. Anal. Chem. 50:576-585.
87. Mrksich, M., Chen, C. S., Xia, Y., Dike, L. E., Ingber, D. E., and Whitesides, G. M. (1996). Controlling cell attachment on contoured surfaces with self-assembled monolayers of alkanethiolates on gold. Proc. Nat. Acad. Sci. 93:10775-10778.
88. Nam, Y., Chang, J. C., Wheeler, B. C., and Brewer, G. J. (2004). Gold-coated microelectrode array with thiol linked self-assembled monolayers for engineering neuronal cultures. IEEE Trans. Biomed. Eng. 51:158-165.
89. Nanci, A., Wuest, J. D., Peru, L., Brunet, P., Sharma, V., Zalzal, S., and McKee, M. D. (1998). Chemical modification of titanium surfaces for covalent attachment of biological molecules. J. Biomed. Mater. Res. 40:324-335.
90. Nelson, K. E., Gamble, L., Jung, L. S., Boeckl, M. S., Naeemi, E., Golledge, S. L., Sasaki, T., Castner, D. G., Campbell, C. T., and Stayton, P. S. (2001). Surface characterization of mixed self-assembled monolayers designed for streptavidin immobilization. Langmuir 17:2807-2816.
91. Nicolelis, M. A. (2001). Actions from thoughts. Nature 409:403-407.
92. Nicolelis, M. A. and Fanselow, E. E. (2002). Dynamic shifting in thalamocortical processing during different behavioural states. Philos. Trans. R. Soc. Lond. B Biol. Sci. 357:1753-1758.
93. Novak, J. L. and Wheeler, B. C. (1986). Recording from the aplysia abdominal ganglion with a planar microelectrode array. IEEE Trans. Biomed. Eng. 33:196-202.
94. Plueddemann, E. P. (1991). Silane Coupling Agents. Plenum Press, New York.
95. Polleux, F., Morrow, T., and Ghosh, A. (2000). Semaphorin 3A is a chemoattractant for cortical apical dendrites. Nature 404:567-573.
96. Prinz, A. and Fromherz, P. (2000). Electrical synapses by guided growth of cultured neurons from the snail Lymnaea stagnalis. Biol. Cyb. 82:L1-L5.
97. Proctor, A., Castner, J. F., Wingard, L. B., Jr., and Hercules, D. M. (1985). Electron spectroscopic (ESCA) studies of platinum surfaces used for enzyme electrodes. Anal. Chem. 57:1644-1649.
98. Ravenscroft, M. S., Bateman, K. E., Shaffer, K. M., Schessler, H. M., Jung, D. R., Schneider, T. W., Montgomery, C. B., Custer, T. L., Schaffner, A. E., Liu, Q. Y., Li, Y. X., Barker, J. L., and Hickman, J. J. (1998). Developmental neurobiology implications from fabrication and analysis of hippocampal neuronal networks on patterned silane-modified surfaces. J. Am. Chem. Soc. 120:12169-12177.
99. Rutten, W. L. C., Smit, J. P. A., Frieswijk, T. A., Bielen, J. A., Brouwer, A. L. H., Buitenweg, J. R., and Heida, C. (1999). Neuro-electronic interfacing with multielectrode arrays. IEEE Eng. Med. Biol. 1999:47-55.
100. Sadana, A. (1992). Protein adsorption and inactivation on surfaces. influence of heterogeneities. Chem. Rev. 92:1799-1818.
101. Schwartz, M. A., Schaller, M. D., and Ginsberg, M. H. (1995). Integrins: Emerging paradigms of signal transduction. Ann. Rev. Cell Dev. Biol. 11:549-599.
102. Shahaf, G. and Marom, S. (2001). Learning in networks of cortical neurons. J. Neurosci. 21:8782-8788.
103. Sheth, S. R. and Leckband, D. (1997). Measurements of attractive forces between proteins and end-grafted poly (ethylene glycol) chains. Proc. Nat. Acad. Sci. 94:8399-8404.
104. Singhvi, R., Kumar, A., Lopez, G. P., Stephanopoulos, G. N., Wang, D. I. C., Whitesides, G. M., and Ingber, D. E. (1994). Engineering cell shape and function. Science 264:696-698.
105. Song, H.-J., Ming, G.-L., He, Z., Lehmann, M., McKerracher, L., Tessier-Lavigne, M., and Poo, M.-M. (1998). Conversion of neuronal growth cone responses from repulsion to attraction by cyclic nucleotides. Science 281:1515-1518.
106. Sorribas, H., Padeste, C., Tiefenauer, L. (2002). Photolithographic generation of protein micropatterns for neuron culture applications. Biomaterials 23:893-900.
107. Stayton, P. S., Olinger, J. M., Bohn, P. W., and Sligar, S. G. (1992). Genetic engineering of surface attachment sites yields oriented protein monolayers. J. Am. Chem. Soc. 114:9298-9299.
108. Stenger, D. A., Hickman, J. J., Bateman, K. E., Ravenscroft, M. S., Ma, W., Pancrazio, J. J., Shaffer, K., Schaffner, A. E., Cribbs, D. H., and Cotman, C. W. (1998). Microlithographic determination of axonal/dendritic polarity in cultured hippocampal neurons. J. Neurosci. Meth. 82:167-173.
109. Stoppini, L., Duport, S., and Correges, P. (1997). A new extracellular multirecording system for electrophysiological studies: Application to hippocampal organotypic cultures. J. Neurosci. Meth. 72:23-33.
110. Taborelli, M., Eng, L., Descouts, P., Ranieri, J. P., Bellamkonda, R., and Aebischer, P. (1995). Bovine serum albumin conformation on methyl and amine functionalized surfaces compared by scanning force microscopy. J. Biomed. Mat. Res. 29:707-714.
111. Takei, K., Shin, R.-M., Inoue, T., Kato, K., and Mikoshiba, K. (1998). Regulation of nerve growth mediated by inositol 1, 4, 5-Triphosphate receptors in growth cones. Science 282:1705-1708.
112. Tao, H. W., Zhang, L. I., Bi, G.-Q., and Poo, M.-M. (2000). Selective presynaptic propagation of long-term potentiation in defined neural networks. J. Neurosci. 20:3233-3243.
113. Thiebaud, P., de Rooij, N. F., Koudelka-Hep, M., and Stoppini, L. (1997). Microelectrode arrays for electrophysiological monitoring of hippocampal organotypic slice cultures. IEEE Trans. Biomed. Eng. 44:1159-1163.
114. Thiebaud, P., Lauer, L., Knoll, W., and Offenhaeusser, A. (2002). PDMS device for patterned application of microfluids to neuronal cells arranged by microcontact printing. Biosens. Bioelectron. 17:87-93.
115. Thomas, C. A., Jr., Springer, P. A., Loeb, G. E., Berwald-Netter, Y., and Okun, L. M. (1972). A miniature microelectrode array to monitor the bioelectric activity of cultured cells. Exp. Cell Res. 74:61-66.
116. Untereker, D. F., Lennox, J. C., Wier, L. M., Moses, P. R., and Murray, R. W. (1977). Chemically modified electrodes Part IV. Evidence for formation of monolayers of bonded organosilane reagents. J. Electroanal. Chem. 81:309-318.
117. Urban, N. and Barrionuevo, G. (1998). Active summation of excitatory postsynaptic potentials in hippocampal CA3 pyramidal neurons. Proc. Nat. Acad. Sci. 95:11450-11455.
118. Vargo, T. G., Thompson, P. M., Gerenser, L. J., Valentini, R. F., Aebischer, P., Hook, D. J., and Gardella, J. A., Jr. (1992). Monolayer chemical lithography and characterization of fluoropolymer films. Langmuir 8:130-134.
119. Venkateswar, R. A., Branch, D. W., and Wheeler, B. C. (2000). An electrophoretic method for microstamping biomolecule gradients. Biomed. Microdev. 2:255-264.
120. Vogt, A. K., Lauer, L., Knoll, W., and Offenhausser, A. (2003). Micropatterned substrates for the growth of functional neuronal networks of defined geometry. Biotechnol. Prog. 19:1562-1568.
121. Vogt, A. K., Stefani, F. D., Best, A., Nelles, G., Yasuda, A., Knoll, W., and Offenhausser, A. (2004). Impact of micropatterned surfaces on neuronal polarity. J. Neurosci. Meth. 134:191-198.
122. Ward, W. K., Jansen, L. B., Anderson, E., Reach, G., Klain, J.-C., and Wilson, G. S. (2002). A new amperometric glucose microsensor: In vitro and short-term in vivo evaluation. Biosens. Bioelectron. 17:181-189.
123. Weetall, H. H. (1976). Covalent coupling methods for inorganic support materials. Meth. Enzymol. 44:134-148.
124. Weiland, J. and Anderson, D. (2000). Chronic neural stimulation with thin-film, iridium oxide electrodes. IEEE Trans. Biomed. Eng. 47:911-918.
125. Wheeler, B. C., and Brewer, G. J. (1994). Multineuron patterning and recording. In: Stenger, D. A. and McKenna, T. M., eds., Enabling Technologies for Cultured Neural Networks. Academic Press, San Diego, pp. 167-185.
126. Wheeler, B. C., Corey, J. M., Brewer, G. J., and Branch, D. W. (1999). Microcontact printing for precise control of nerve cell growth in culture. J. Biomech. Eng. 121:73-78.
127. Wise, K. D. and Weissman, R. H. (1971). Thin films of glass and their application to biomedical sensors. Med. Biol. Eng. 9:339-350.
128. Xia, Y. and Whitesides, G. M. (1998). Soft lithography. Angew. Chem. Int. Ed. 37:550-575.
129. Xiao, Z.-C., Ragsdale, D. S., Malhotra, J. D., Mattei, L. N., Braun, P. E., Schachner, M., and Isom, L. L. (1999). Tenascin-R is a functional modulator of sodium channel β subunits. J. Biol. Chem. 274:26511-26517.
130. Zhang, M., Desai, T., and Ferrari, M. (1998). Proteins and cells on PEG immobilized silicon surfaces. Biomaterials 19:953-960.
131. Zhao, X.-M., Wilbur, J. L., and Whitesides, G. M. (1996). Using two-stage chemical amplification to determine the density of defects in self-assembled monolayersofalkanethiolates on gold. Langmuir 12:3257-3264.