Directed evolution of a Baeyer-Villiger monooxygenase to enhance enantioselectivity

Applied Microbiology and Biotechnology

Volumn 81, Issue 3, 2008, Pages 465-472

  Kirschner, Anett ^a,b   Bornscheuer, Uwe T ^a  

^a UNIVERSITY OF GREIFSWALD   (Germany)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

hydroxyketones; Baeyer Villiger monooxygenase; Directed evolution; Enantioselectivity; Enzyme catalysis

Indexed keywords

ABS RESINS; BOTTLES; ELECTRON TRANSITIONS; ENZYMES; KETONES; ORGANIC COMPOUNDS;

ACYCLIC KETONES; BAEYER-VILLIGER MONOOXYGENASE; CONSECUTIVE CYCLES; DIRECTED EVOLUTION; DIRECTED EVOLUTIONS; DOUBLE MUTANTS; ENANTIOSELECTIVELY; ENZYME CATALYSIS; KINETIC RESOLUTIONS; MONOOXYGENASE; POLYMERASE CHAIN REACTIONS; PSEUDOMONAS FLUORESCENS; RANDOM MUTATIONS; REACTION CONDITIONS; SCREENING METHODS; SHAKE FLASKS; WILD TYPES;

ENANTIOSELECTIVITY;

4 HYDROXY 2 KETONE; ACETIC ACID DERIVATIVE; HYDROXYALKYL ACETATE; KETONE DERIVATIVE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

BIOTRANSFORMATION; ENZYME ACTIVITY; EVOLUTION; KETONE; MUTATION; OPTIMIZATION; OXIDATION;

ARTICLE; BAEYER VILLIGER REACTION; BIOTRANSFORMATION; ENANTIOSELECTIVITY; GENE MUTATION; KINETICS; MOLECULAR CLONING; OXIDATION; POLYMERASE CHAIN REACTION; PSEUDOMONAS FLUORESCENS; SCREENING; WILD TYPE;

BACTERIAL PROTEINS; DIRECTED MOLECULAR EVOLUTION; KETONES; KINETICS; MIXED FUNCTION OXYGENASES; MUTAGENESIS, SITE-DIRECTED; PSEUDOMONAS FLUORESCENS; SUBSTRATE SPECIFICITY;

PSEUDOMONAS FLUORESCENS;

EID: 56349141488     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1646-4     Document Type: Article

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