Characterization and enantioselectivity of a recombinant esterase from Pseudomonas fluorescens

Enzyme and Microbial Technology

Volumn 22, Issue 7, 1998, Pages 641-646

  Krebsfanger N ^a   Zocher, F ^a   Altenbuchner, J ^a   Bornscheuer, U T ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Enantioselectivity; Esterase; Pseudomonas fluorescens; Purification

Indexed keywords

AROMATIC COMPOUNDS; CATALYST ACTIVITY; CELL CULTURE; COLIFORM BACTERIA; ESTERS; ETHANOL; HYDROLYSIS; PH EFFECTS; PURIFICATION; THERMAL EFFECTS;

ACETATE; ENANTIOSELECTIVITY; ESTERASE; PROPANOL;

ENZYMES;

ACETIC ACID; ALCOHOL; ESTER; ESTERASE; LONG CHAIN FATTY ACID; RECOMBINANT ENZYME;

ARTICLE; ENANTIOMER; ESCHERICHIA COLI; NONHUMAN; PH; PSEUDOMONAS FLUORESCENS; TEMPERATURE;

EID: 0032524178     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(98)00004-0     Document Type: Article

References (17)

1. Wong C.H., Whitesides G.M. Enzymes in Synthetic Organic Chemistry. 1994;Pergamon Press, Oxford.
2. Zhu L.M., Tedford M.C. Applications of pig liver esterases (PLE) in asymmetric synthesis. Tetrahedron. 46:1990;6587-6611.
3. Toone E.J., Werth M.J., Jones J.B. Active-site model for interpreting and predicting the specificity of pig liver esterase. J. Am. Chem. Soc. 112:1990;4946-4952.
4. Choi K.D., Jeohn G.H., Rhee J.S., Yoo O.J. Cloning and nucleotide sequence of an esterase gene from Pseudomonas fluorescens and expression of the gene in Escherichia coli. Agric. Biol. Chem. 54:1990;2039-2045.
5. Nishizawa M., Shimizu M., Ohkawa H., Kanaoka M. Stereoselective production of (+)-trans-chrysanthemic acid by a microbial esterase Cloning, nucleotide sequence, and overexpression of the esterase gene of Arthrobacter globiformis in Escherichia coli . Appl. Environ. Microbiol. 61:1995;3208-3215.
6. Molinari F., Brenna O., Valenti M., Aragozzini F. Isolation of a novel carboxylesterase from Bacillus coagulans with high enantioselectivity toward racemic esters of 1,2-O-isopropylideneglycerol. Enzyme Microb. Technol. 19:1996;551-556.
7. Nishizawa M., Gomi H., Kishimoto F. Purification and some properties of carboxylesterase from Arthrobacter globiformis Stereoselective hydrolysis of ethyl chrysanthemate . Biosci. Biotechnol. Biochem. 57:1993;594-598.
8. Azzolina O., Collina S., Vercesi D. Stereoselective hydrolysis by esterase A strategy for resolving 2-(R,R′-phenoxy)propionyl ester racemates . II Farmaco. 50:1995;725-733.
9. Pelletier I., Altenbuchner J. A bacterial esterase is homologous with non-haem haloperoxidases and displays brominating activity. Microbiology. 141:1995;459-468.
10. Volff J.N., Eichenseer C., Viell P., Piendl W., Altenbuchner J. Nucleotide sequence and role in DNA amplification of the direct repeats composing the amplifiable element AUD1 of Streptomyces lividans 66. Mol. Microbiol. 21:1996;1037-1047.
11. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein assay utilizing the principle of protein dye-binding. Anal. Biochem. 72:1976;248-254.
12. Bornscheuer U.T., Altenbuchner J., Meyer H.H. Directed evolution of an esterase for the stereoselective resolution of a key intermediate in the synthesis of epothilones. Biotechnol. Bioeng. 58:1998;554-559.
13. Enzelberger M.M., Bornscheuer U.T., Gatfield I., Schmid R.D. Lipase-catalysed resolution of γ- and δ-lactones. J. Biotechnol. 56:1997;129-133.
14. Chen C.S., Fujimoto Y., Girdaukas G., Sih C.J. Quantitative analyses of biochemical kinetic resolutions of enantiomers. J. Am. Chem. Soc. 104:1982;7294-7299.
15. Ley S.V., Parra M., Redgrave A.J., Sternfeld F. Microbial oxidation in synthesis Preparation of myo-inositol phosphates and related cyclitol derivatives from benzene . Tetrahedron. 46:1990;4995-5026.
16. Zadel G., Rieger R., Breitmaier E. Enantioselective terpene syntheses by Diels-Alder reaction of 1-(1-arylalkoxy)-2-methyl-1-1,3-butadiene with isoprene. Liebigs Ann. Chem. 1991;1343-1346.
17. Bornscheuer U., Reif O.-W., Lausch R., Freitag R., Scheper R., Kolisis F.N., Menge U. Lipase of Pseudomonas cepacia for biotechnological purposes Purification, crystallization, and characterization . Biochim. Biophys. Acta. 1201:1994;55-60.