pPIC9-Fc: A Vector System for the Production of Single-Chain Fv-Fc Fusions in Pichia pastoris as Detection Reagents in Vitro

Journal of Biochemistry

Volumn 134, Issue 6, 2003, Pages 911-917

  Liu, Jianglan ^a   Wei, Dongzhi ^a   Qian, Feng ^a   Zhou, Yuxun ^a   Wang, Jinzhi ^a   Ma, Yushu ^a   Han, Zeguang ^b  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b CHINESE NATIONAL HUMAN GENOME CENTER AT SHANGHAI   (China)

Author keywords

Antibody engineering; Detection reagent; Fc fragment; Pichia pastoris; Single chain Fv

Indexed keywords

DIMER; GLUTATHIONE TRANSFERASE; HISTIDINE; IMMUNOGLOBULIN G1; REAGENT; RECOMBINANT ANTIBODY; SINGLE CHAIN FC FRAGMENT ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG;

ANTIBODY ENGINEERING; ARTICLE; COLUMN CHROMATOGRAPHY; CULTURE MEDIUM; EXPRESSION VECTOR; FERMENTATION; FUNGUS CULTURE; FUSION GENE; GENE CASSETTE; IMMUNOASSAY; MOLECULAR CLONING; NONHUMAN; PICHIA PASTORIS; PPIC9 FC EXPRESSION VECTOR; PROTEIN BINDING; REACTOR;

ANIMALS; BINDING SITES, ANTIBODY; BLOTTING, WESTERN; CLONING, MOLECULAR; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, FUNGAL; GENETIC VECTORS; IMMUNOGLOBULIN FC FRAGMENTS; MICE; MUTAGENESIS, INSERTIONAL; PICHIA; RECOMBINANT FUSION PROTEINS;

FUNGI; PICHIA; PICHIA PASTORIS; SCHISTOSOMA JAPONICUM;

EID: 1342304265     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/jb/mvg222     Document Type: Article

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