Cloning, expression, and characterization of Babesia gibsoni dihydrofolate reductase-thymidylate synthase: Inhibitory effect of antifolates on its catalytic activity and parasite proliferation

Antimicrobial Agents and Chemotherapy

Volumn 52, Issue 11, 2008, Pages 4072-4080

  Aboge, Gabriel O ^a   Jia, Honglin ^a   Terkawi, Mohamad A ^a   Goo, Youn Kyoung ^a   Nishikawa, Yoshifumi ^a   Sunaga, Fujiko ^b   Namikawa, Kuzuhiko ^b   Tsuji, Naotoshi ^c   Igarashi, Ikuo ^a   Suzuki, Hiroshi ^a   Fujisaki, Kozo ^a,d   Xuan, Xuenan ^a  

^a OBIHIRO UNIVERSITY OF AGRICULTURE AND VETERINARY MEDICINE   (Japan)

^b AZABU UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

^d KAGOSHIMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DIHYDROFOLATE REDUCTASE; DIHYDROFOLIC ACID; FOLIC ACID ANTAGONIST; GLUTATHIONE TRANSFERASE; METHOTREXATE; POLYPEPTIDE; PYRIMETHAMINE; RECOMBINANT ENZYME; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; THYMIDYLATE SYNTHASE; TRIMETHOPRIM;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BABESIA; BABESIA GIBSONI; CONTROLLED STUDY; DHFR TS GENE; DOG; DRUG POTENCY; DRUG TARGETING; ENZYME ACTIVE SITE; ENZYME INHIBITION; ENZYME MECHANISM; ESCHERICHIA COLI; FEMALE; GENE; GENE EXPRESSION; GENE ISOLATION; IC 50; IN VITRO STUDY; MALE; MEROZOITE; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; WILD TYPE;

AMINO ACID SEQUENCE; ANIMALS; ANTIPROTOZOAL AGENTS; BABESIA; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; DNA, PROTOZOAN; ESCHERICHIA COLI; FOLIC ACID ANTAGONISTS; GENE EXPRESSION; GENES, PROTOZOAN; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; TETRAHYDROFOLATE DEHYDROGENASE; THYMIDYLATE SYNTHASE;

EID: 55849120501     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.00384-08     Document Type: Article

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