DNA damage in workers occupationally exposed to pesticide mixtures

Journal of Applied Toxicology

Volumn 28, Issue 8, 2008, Pages 957-965

  Simoniello, M F ^a   Kleinsorge, E C ^a   Scagnetti, J A ^a   Grigolato, R A ^a   Poletta, G L ^a,b   Carballo, M A ^b  

^a UNIVERSIDAD NACIONAL DEL LITORAL   (Argentina)

^b UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

Comet assay; Farmworkers; Genotoxicity; Human biomonitoring; Occupational exposure; Pesticide applicators

Indexed keywords

AGRICULTURAL CHEMICAL; CARCINOGEN; PESTICIDE;

ADULT; ALCOHOL CONSUMPTION; ARGENTINA; ARTICLE; CONTROL GROUP; CONTROLLED STUDY; CULTIVATED LAND; DEVELOPING COUNTRY; DNA DAMAGE; ENVIRONMENTAL EXPOSURE; FEMALE; GENOTOXICITY; HEALTH HAZARD; HUMAN; INDUSTRIAL WORKER; MAJOR CLINICAL STUDY; MALE; NEOPLASM; OCCUPATIONAL EXPOSURE; PRIORITY JOURNAL; PROTECTIVE EQUIPMENT; SMOKING;

ADULT; AGRICULTURAL WORKERS' DISEASES; ARGENTINA; CELL SURVIVAL; COMET ASSAY; COMPLEX MIXTURES; DNA DAMAGE; FEMALE; FLUORESCENT DYES; HUMANS; LIFE STYLE; MALE; MIDDLE AGED; OCCUPATIONAL EXPOSURE; PESTICIDES; RISK;

EID: 55649111603     PISSN: 0260437X     EISSN: 10991263     Source Type: Journal    
DOI: 10.1002/jat.1361     Document Type: Article

References (76)

1. Acquavella J, Doe J, Tomenson J, Chester G, Cowell J, Bloemen L. 2003. Epidemiologic studies of occupational pesticide exposure and cancer: regulatory risk assessments and biologic plausibility. Ann. Epidemiol. 13: 1-7.
2. Albertini RJ, Anderson D, Douglas GR, Hagmar L, Hemminki K, Merlo F, Natarajan AT, Norppa H, Shuker DE, Tice R, Waters MD, Aitio A. 2000. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. International Programme on Chemical Safety. Mutat. Res. 463: 111-172.
3. Arbuckle TE, Sever LE. 1998. Pesticide exposures and fetal death: a review of the epidemiologic literature. Crit. Rev. Toxicol. 28: 229-270.
4. Barr DB, Needham L. 2002. Analytical methods for biological monitoring of exposure to pesticides: a review. J. Chromatogr. B 778: 5-29.
5. Bhalli J, Khan QM, Haq MA, Khalid AM, Nasim A. 2006. Cytogenetic analysis of Pakistani individuals occupationally exposed to pesticides in a pesticide production industry. Mutagenesis 21: 143-148. DOI: 10.1093/mutagen/ge1009.
6. Bolognesi C. 2003. Genotoxicity of pesticides: a review of human biomonitoring studies. Mutat. Res. 543: 251-272.
7. Bolognesi C, Parrini M, Merlo F, Bonassi S. 1993. Frequency of micronuclei in lymphocytes from a group of floriculturists exposed to pesticides. J. Toxicol. Environ. Hlth 40: 405-411.
8. Bolognesi C, Perrone E, Landini E. 2002. Micronucleus monitoring of a floriculturist population from western Liguria, Italy. Mutagenesis 17: 391-397.
9. Bolognesi C, Landini E, Perrone E, Roggieri P. 2004. Cytogenetic biomonitoring of a floriculturist population in Italy: micronucleus analysis by fluorescence in situ hybridization (FISH) with an all-chromosome centromeric probe. Mutat. Res. 557: 109-117.
10. Bowden RD, Buckwalter MR, McBride JF, Johnson DA, Murray BK, O'Neill KL. 2003. Tail profile: a more accurate system for analyzing DNA damage using the Comet assay. Mutat. Res. 537: 1-9.
11. Bull S, Fletcher K, Boobis AR, Battershill JM. 2006. Evidence for genotoxicity of pesticides in pesticide applicators: a review. Mutagenesis 21: 93-103. DOI: 10.1093/mutagen/gel011.
12. Burlinson B, Tice RR, Speit G, Agurell E, Brendler-Schwaab SY, Collins AR, Escobar P, Honma M, Kumaravel TS, Nakajima M, Sasaki YF, Thybaud V, Uno Y, Vasquez M, Hartmann A. 2007. Fourth International Workgroup on Genotoxicity testing: results of the In Vivo Comet Assay Workgroup. Mutat. Res. 627: 31-35. DOI: 10.1016/j.mrgentox.2006.08.011.
13. Carbonell E, Puig M, Xamena N, Creus A, Marcos R. 1990. Sister chromatid exchange in lymphocytes of agricultural workers exposed to pesticides. Mutagenesis 5: 403-405.
14. Carbonell E, Valbuena A, Xamena N, Creus A, Marcos R. 1995. Temporary variations in chromosomal aberrations in a group of agricultural workers exposed to pesticides. Mutat. Res. 344: 127-134.
15. Castillo-Cadena J, Tenorio-Vieyra LE, Quintana-Carabia AI, Garcia-Fabila MM, Ramirez-San Juan E, Madrigal-Bujaidar E. 2006. Determination of DNA Damage in Floriculturists Exposed to Mixtures of Pesticides. J. Biomed. Biotechnol. 2006: 1-12. DOI: 10.1155/JBB/2006/97896.
16. COM. 2000. United Kingdom Committee on Mutagenicity of Chemicals in Food, Consumer Products, and the Environment. Guidance on a Strategy for Testing of Chemicals for Mutagenicity. http://www.doh.gov.uk/com/guidance.pdf.
17. Coronado GD, Thompson B, Strong L, Griffith WC, Islas I. 2004. Agricultural task and exposure to organophosphate pesticides among farmworkers. Environ. Hlth Perspect. 112: 142-147. DOI: 10.1289/ehp.6412.
18. Costa C, Teixeira J, Silva S, Roma-Torres J, Coelho P, Gaspar J, Alves M, Laffon B, Rueff J, Mayan O. 2006. Cytogenetic and molecular biomonitoring of a Portuguese population exposed to pesticides. Mutagenesis 21: 343-350. DOI: 10.1093/mutagen/ge1039.
19. Curl C, Fenske R, Kissel J, Shirai J, Moate T, Griffith W, Coronado G, Thompson B. 2002. Evaluation of take-home organophosphorus pesticide exposure among agricultural workers and their children. Environ. Hlth Perspect. 110: A787-A792.
20. De Marco A, De Salvia R, Polani S, Ricordy R, Sorrenti F, Perticone P, Cozzi R, D'Ambrosio C, De Simone C, Guidotti M, Albanesi T, Duranti G, Festa F, Gensabella G, Owczarek M. 2000. Evaluation of genotoxic and cytotoxic properties of pesticides employed in italian agricultural practices. Environ. Res. A 83: 311-321.
21. De Roos A, Blair A, Rusiecki J, Hoppin J, Svec M, Dosemeci M, Sandler D, Alavanja M. 2005. Cancer Incidence among glyphosate-exposed pesticide applicators in the agricultural health study. Environ Hlth Perspect. 113: 49-54.
22. Dulout FN, Pastori MC, Olivero OA, Gonzalez Cid M, Loria D, Matos E, Sobel N, de Bujan EC, Albiano N. 1985. Sisterchromatid exchanges and chromosomal aberrations in a population exposed to pesticides. Mutat. Res. 143: 237-244.
23. Engel L, Checkoway H, Keifer M, Seixas N, Longstreth W, Scott K, Hudnell K, Anger W, Camicioli R. 2001. Parkinsonism and occupational exposure to pesticides. Occup. Environ. Med. 58: 581-589.
24. Eskenazi B, Harley K, Bradman A, Weltzien E, Jewell N, Barr D, Furlong C, Holland N. 2004. Association of in utero organophosphate pesticide exposure and fetal growth and length of gestation in an agricultural population. Environ. Hlth Perspect. 112(10): 1116-1124. DOI: 10.1289/ehp.6789.
25. Faust F, Kassie F, Knasmüller S, Kevekordes S, Mersch-Sundermann V. 2004. Use of primary blood cells for the assessment of exposure to occupational genotoxicants in human biomonitoring studies. Toxicology 198: 341-350.
26. FDA. Guidance for Industry and Review Staff. Recommended Approaches to Integration of Genetic Toxicology Study Results. http://www.fda.gov/cder/ guidance/index.htm [2006].
27. Garaj-VrhovacV, Zeljezic D. 2000 Evaluation of DNA damage in workers occupationally exposed to pesticides using single cell gel electrophoresis (SCGE) assay. Pesticide genotoxicity relevealed by Comet assay. Mutat. Res. 469: 279-285.
28. Garaj-Vrhovac V, Zeljezic D. 2001. Cytogenetic monitoring of croatian population occupationally exposed to a complex mixture of pesticides. Toxicology 165: 153-162.
29. Gauthier E, Forthier I, Gourchesne F, Pepin P, Mortimer J, Gauvreau D. 2001. Environmental pesticide exposure as a risk factor for Alzheimer's disease: a case control study. Environ. Res. 86: 37-45.
30. Gomez-Arroyo S, Noriega-Aldana N, Osorio A, Galicia F, Ling S, Villalobos-Pietrini R. 1992. Sister-chromatid exchange analysis in a rural population of Mexico exposed to pesticides. Mutat. Res. 281: 173-179.
31. Gomez-Arroyo S, Diaz-Sanchez Y, Meneses-Perez MA, Villalobos-Pietrini R, De Leon-Rodriguez J. 2000. Cytogenetic biomonitoring in a Mexican floriculture worker group exposed to pesticides. Mutat. Res. 466: 117-124.
32. Groten JP. 2000. Mixtures and interactions. Food Chem. Toxicol. 38(1 suppl.): S65-S71.
33. Grover P, Danadevi K, Mahbood M, Rozati R, Saleha Banu B, Rahman MF. 2003. Evaluation of genetic damage in workers employed in pesticide production utilizing the Comet assay. Mutagenesis 18: 201-205.
34. Hoyos LS, Carvajal S, Solano L, Rodríguez J, Orozco L, López Y, Au WW. 1996. Cytogenetic Monitoring of Farmers Exposed to Pesticides in Colombia. Environ. Hlth Perspect. 104: 535-538.
35. IRAC. International Agency for Research on Cancer. Agents reviewed by the IARC Monographs. Vols 1-88. IARC, Lyon. http://monographs.iarc.fr [2008].
36. Islas-Gonzalez K, Gonzalez-Horta C, Sánchez-Ramirez B, Reyes-Aragon E, Levario-Carrillo M. 2005. In vitro assessment of the genotoxicity of ethyl paraoxon in newborns and adults. Hum. Exp. Toxicology 24: 319-324.
37. Jaga K, Dharmani C. 2005. Epidemiology of pesticide exposure and cancer: a review. Rev. Environ. Hlth 20: 15-38.
38. Jakubowski M, Trzcinka-Ochocka M. 2005. Biological Monitoring of exposure: trends and key developments. J. Occup. Hlth 47: 22-48.
39. Jenner P. 2001. Parkinson's disease, pesticides and mitochondrial dysfunction. Trends Neurosci. 24: 245-247.
40. Ji BT, Silverman DT, Steward PA, Blair A. 2001. Occupational exposure to pesticides and pancreatic cancer. Am. J. Indust. Med. 39: 92-99.
41. Karalliedde LD, Edwards P, Marrs TC. 2003.Variables influencing the toxic response to organophosphates in humans. Food Chem. Toxicol. 41: 1-13.
42. Klein SM, Cohen G, Cederbaum A. 1981. Production of formaldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical generating system. Biochemistry 20: 6006-6012.
43. Kourakis A, Mouratidou M, Kokkinos G, Barbouti A, Kotsis A, Mourelatos D, Dozi-Vassiliades J. 1992. Frequencies of chromosomal aberrations in pesticide sprayers working in plastic green houses. Mutat. Res. 279: 145-148.
44. La Vechia C, Negri E, d'Evanzo B, Francheschi S. 1989 Occupation and lymphoid neoplasms. Br. J. Cancer 60: 385-388.
45. Lander BF, Knudsen LE, Gamborg MO, Jarventaus H, Norppa H. 2000. Chromosome aberrations in pesticide-exposed greenhouse workers. Scand. J. Work Environ. Hlth 26: 436-442.
46. Lebailly P, Vigreux C, Godard E, Deslandes F, Sichel JY, Le Talaër M, Henry-Amar, Gauduchon P. 1997. Use of alkaline Comet assay to detect DNA damages in mononuclear leukocytes of farmers occupationally exposed to pesticides. Fundam. Mol. Mech. Mutagen. 379(1, suppl. 1): S157.
47. Lebailly P, Vigreux C, Lechevrel C, Ledemeney D, Godard T, Sichel F, Le Talaër JY, Henry-Amar M, Gauduchon P. 1998a. Damage in mononuclear leukocytes of farmers measured using the alkaline comet assay: modifications of DNA Damage levels after a one-day field spraying period with selected pesticides. Cancer Epidemiol. Biomarkers Prev. 7: 929-940.
48. Lebailly P, Vigreux C, Lechevrel C, Ledemeney D, Godard T, Sichel F, Le Talaër JY, Henry-Amar M, Gauduchon P. 1998b. DNA damage in mononuclear leukocytes of farmers measured using the alkaline comet assay: discussion of critical parameters and evaluation of seasonal variations in relation to pesticide exposure. Cancer Epidemiol. Biomarkers Prev. 7: 917-927.
49. Liu YJ, Huang PL, Chang YF, Chen YH, Chiou YH, Xu ZL, Wong RH. 2006. GSTP1 genetic polymorphism is associated with a higher risk of DNA damage in pesticide-exposed fruit growers. Cancer Epidemiol. Biomarkers Prev. 15(4): 659-666. DOI: 10.1158/1055-9965.EPI-05-0617.
50. Lucero L, Pastor L, Suarez S, Durbán R, Gómez C, Parrón T, Creus A, Marcos R. 2000. Cytogenetic biomonitoring of Spanish greenhouse workers exposed to pesticides: micronuclei analysis in peripheral blood lymphocytes and buccal epithelial cells. Mutat. Res. 464: 255-262.
51. Mercille S, Massie B. 1994. Induction of apoptosis in nutrient-deprived cultures of hybridoma and myeloma cells. Biotechnol. Bioengng 44: 1140-1154.
52. Moller P, Knudsen LE, Loft S, Wallin H. 2000. The Comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors. Cancer Epidemiol. Biomarkers Prev. 9: 1005-1015.
53. Olive PL. 1989. Cell proliferation as a requirements for development of the contact effect in Chinese hamster V79 spheroids. Radiat. Res. 117: 79-92.
54. Palacios Nava ME. 2003. Aplicación de un instrumento para evaluar exposición a plaguicidas órganofosforados, efectos agudos y subagudos en la salud de trabajadores agrícolas. Rev. Fac. Med. UNAM. 46(1): 22-27.
55. Palus J, Dziubaltowska E, Rydzynski K. 1999. DNA damage detected by the Comet assay in the white blood cells of workers furniture plant. Mutat. Res. 444: 61-74.
56. Pastor S, Gutierrez S, Creus A, Cebulska-Wasilewska A, Marcos R. 2001a. Micronuclei in peripheral blood lymphocytes and buccal epithelial cells of Polish farmers exposed to pesticides. Mutat. Res. 495: 147-156.
57. Pastor S, Gutierrez S, Creus A, Xamena N, Piperakis S, Marcos R. 2001b. Cytogenetic analysis of Greek farmers using the micronucleus assay in peripheral lymphocytes and buccal cells. Mutagenesis 16: 539-545.
58. Pastor S, Creus A, Xamena N, Siffel C, Marcos R. 2002. Occupational exposure to pesticides and cytogenetic damage: results of a Hungarian population study using the micronucleus assay in lymphocytes and buccal cells. Environ. Mol. Mutagen. 40: 101-109.
59. Paz-y-Miño C, Bustamante G, Sánchez ME, Leone PE. 2002. Cytogenetic monitoring in a population occupationally exposed to pesticides in Ecuador. Environ. Hlth Perspect. 110: 1077-1080.
60. Peluso M, Merlo F, Munnia A, Bolognesi C, Puntoni R, Parodi S. 1996. (32)P-postlabeling detection of DNA adducts in peripheral white blood cells of greenhouse floriculturists from western Liguria, Italy. Cancer Epidemiol. Biomarkers Prev. 5: 361-369.
61. Petrelli G, Figa-Talamanca I, Tropeano R, Tangucci M, Cini C, Aquilini S, Gasperini L, Meli P. 2000. Reproductive male mediated risk: spontaneous abortion among wives of pesticide applicators. Eur. J. Epidemiol. 16: 391-393.
62. Piperakis SM, Petrakou E, Tsilimigaki S, Sagnou M, Monogiudis E, Haniotakis G, Karkaseli H, Sarikaki E. 2003. Biomonitoring with the Comet assay of Greek greenhouse workers exposed to pesticides. Environ. Mol. Mutagen. 41: 104-110.
63. Piperakis SM, Kontogianni K, Piperakis MM, Tsilimigaki S. 2006. Effects of pesticides on occupationally exposed humans. Sci. World J. 6: 1211-1220. DOI: 10.1100/tsw.2006.207.
64. Preston J. 2003. Molecular epidemiology: potential impacts on the assessment of public health. Mutat. Res. 543: 121-124.
65. Rodríguez Ferreiro G, Cancino Badias L, López-Nigro M, Palermo A, Mudry M, Gonzalez Elio P, Carballo MA. 2002. DNA single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivates. Toxicol. Lett. 132: 109-115.
66. Rojas E, Lopez MC, Valverde M. 1999. Review. Single cell gel electrophoresis assay: methodology and applications. J. Chromatogr. B 722: 225-254.
67. Sailaja N, Chandrasekhar M, Rekhadevi PV, Mahbood M, Rahman MF, Vuyyuri SB, Danadevi K, Hussain SA, Grover P. 2006. Genotoxic evaluation of workers employed in pesticide production. Mutat. Res. 609: 74-80. DOI: 10.1016/j.mrgentox.2006.06.022.
68. Scarpato R, Migliore L, Angotzi G, Fedi A, Miligi L, Loprieno N. 1996. Cytogenetic monitoring of a group of Italian floriculturists: no evidence of DNA damage related to pesticide exposure. Mutat. Res. 367: 73-82.
69. Singh NP, Khan A. 1995. Acetaldehyde: genotoxicity and citotoxicity in human lymphocytes. Mutat. Res. 337: 9-17.
70. Singh NP, McCoy MT, Tice RR, Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individuals cells. Exp. Cell. Res. 175(1): 184-191.
71. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35: 206-221.
72. Titenko-Holland N, Windham G, Kolachana P, Reinisch F, Parvatham S, Osorio AM, Smith MT. 1997. Genotoxicity of malathion in human lymphocytes assessed using the micronucleus assay in vitro and in vivo: A study of malathion-exposed workers. Mutat. Res. 338: 85-95.
73. USEPA. 1998. Research Plan for Endocrine Disruptors. Office of Research and Development. Washington, DC. EPA/600/R-98/087.
74. WHO. Recommended Classification of Pesticides Bay Hazard and Guidelines to Classification. http://www.int/ipcs/publications/ pesticides_hazards/en [2004].
75. Woodward G. 2001. Autism and Parkinson's diseases. Med. Hypotheses 56: 246-249.
76. Zeljezic D, Garaj-Vrhovac V. 2001.Chromosomal aberration and single cell gel electrophoresis (Comet) assay in the longitudinal risk assessment of occupational exposure to pesticides. Mutagenesis 16: 359-363.