Evaluation of DNA damage in workers occupationally exposed to pesticides using single-cell gel electrophoresis (SCGE) assay. Pesticide genotoxicity revealed by comet assay

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 469, Issue 2, 2000, Pages 279-285

  Garaj Vrhovac, Vera ^a   Zeljezic, Davor ^a  

^a INSTITUTE FOR MEDICAL RESEARCH AND OCCUPATIONAL HEALTH   (Croatia)

Author keywords

Comet assay; DNA damage; Human biomonitoring; Pesticides

Indexed keywords

PESTICIDE;

ADULT; ARTICLE; BIOLOGICAL MONITORING; CLINICAL ARTICLE; COMET ASSAY; CONTROLLED STUDY; DNA DAMAGE; DNA REPAIR; GENOTOXICITY; HUMAN; HUMAN CELL; OCCUPATIONAL EXPOSURE; PRIORITY JOURNAL;

ADULT; CASE-CONTROL STUDIES; COMET ASSAY; DNA DAMAGE; DNA REPAIR; EVALUATION STUDIES; FEMALE; HUMANS; LYMPHOCYTES; MALE; MIDDLE AGED; MUTAGENS; OCCUPATIONAL EXPOSURE; PESTICIDES;

EID: 0034692624     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1383-5718(00)00092-9     Document Type: Article

References (34)

1. Östling O., Johanson K.J. Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 123:1984;291-298.
2. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175:1988;184-191.
3. Olive P.L. Cell proliferation as a requirement for development of the contact effect in Chinese hamster V79 spheroids. Radiat. Res. 117:1989;79-92.
4. Collins A., Dušinska M., Franklin M., Samorovska M., Petrovska H., Duthie S., Fillion L., Panayiotidis M., Rašlova K., Vaughan N. Comet assay in human biomonitoring studies: reliability validation, and applications. Environ. Mol. Mutagen. 30:1997;139-146.
5. Betti C., Davini T., Giannessi L., Loprieno N., Barale R. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat. Res. 307:1994;323-333.
6. Betti C., Davini T., Giannessi L., Loprieno N., Barale R. Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects. Mutat. Res. 343:1995;201-207.
7. Ribas G., Frenzilli G., Barale R., Marcos R. Herbicide-induced DNA damage in human lymphocytes evaluated by the single-cell gel electrophoresis (SCGE) assay. Mutat. Res. 344:1995;41-54.
8. R.R. Tice, The single-cell gel/comet assay: A microgel electrophoretic technique for the detection of DNA damage and repair in individual cells, in: D.H. Phillips, S. Venitt (Eds.), Environmental Mutagenesis, BIOS Scientific Publishing, Oxford, UK, 1995, pp. 315-319.
9. Clements C., Ralph S., Petras M. Genotoxicity of selected herbicides in Rana catesbeiana tadpoles using alkaline single-cell gel DNA electrophoresis (comet) assay. Environ. Mol. Mutagen. 29:1997;277-288.
10. Frenzilli G., Betti C., Davini T., Desideri M., Fornai E., Giannessi L., Maggiorelli F., Paoletti P., Barale R. Evaluation of DNA damage in leukocytes of ex-smokers by single-cell gel electrophoresis. Mutat. Res. 375:1997;117-123.
11. Sasaki Y.F., Izumiyama F., Nishidate E., Matsusaka N., Tsuda S. Detection of rodent liver carcinogen genotoxicity by alkaline single-cell gel electrophoresis (comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow). Mutat. Res. 391:1997;201-214.
12. Sasaki Y.F., Nishidate E., Izumiyama F., Matsusaka N., Tsuda S. Simple detection of chemical mutagens by alkaline single-cell gel electrophoresis (comet) assay in multiple mouse organs (liver, lung, spleen, kidney, and bone marrow). Mutat. Res. 391:1997;215-231.
13. Garaj-Vrhovac V., Zeljezic D. Chromosomal aberrations and frequency of micronuclei in workers employed in pesticide production. Biologia. 54(6):1999;707-712.
14. Šram R.J., Podrazilova K., Dejmek J., Mračkova G., Pilčik T. Single-cell gel electrophoresis assay: sensitivity of peripheral white blood cells in human population studies. Mutagenesis. 13:1998;99-103.
15. King J.S., Valgargel E.R., Rufer J.T., Phillips J.W., Moran W.F. Noncomplementary DNA double strand break rejoining in bacterial and human cells. Nucleic Acids Res. 21:1993;1055-1059.
16. Fairbairn D.W., Olive P.L., O'Neill K.L. The comet assay: a comprehensive review. Mutat. Res. 339:1995;37-59.
17. 32P -postlabeling Mol. Cell Biochem. 169:1997;177-184.
18. Ribas G., Surralles J., Carbonell E., Xamena N., Creus A., Marcos R. Genotoxicity of the herbicides alachlor and maleic hydrazide in cultured human lymphocytes. Mutagenesis. 11(3):1996;221-227.
19. Kevekordes S., Gebel T., Pav K., Edenharder R., Dunkelberg H. Genotoxicicty of selected pesticides in the mouse bone-marrow micronucleus test and in the sister-chromatid exchange test with human lymphocytes in vitro. Toxicol. Lett. 89:1996;35-42.
20. Gebel T., Kevekordes S., Pav K., Edenharder R., Dunkelberg H. In vivo genotoxicity of selected herbicides in the mouse bone-marrow micronucleus test. Arch. Toxicol. 71:1997;193-197.
21. Dunkelberg H., Fuchs J., Hengstler J.G., Klein E., Oesch F., Struder K. Genotoxic effects of the herbicides alachlor, atrazine, pendimethaline, and simazine in mammalian cells. Bull. Environ. Contam. Toxicol. 52:1994;498-504.
22. Carbonell E., Puig M., Xamena N., Creus A., Marcos R. Sister chromatid exchange in lymphocytes of agricultural workers exposed to pesticides. Mutagenesis. 5(4):1990;403-405.
23. Carbonell E., Valbuena A., Xamena N., Creus A., Marcos R. Temporary variations in chromosomal aberrations in a group of agricultural workers exposed to pesticides. Mutat. Res. 344:1995;127-134.
24. Kourakis A., Mouratidou M., Barbouti A., Dimikiotou M. Cytogenetic effects of occupational exposure in the peripheral blood lymphocytes of pesticide sprayers. Carcinogenesis. 17(1):1996;99-101.
25. Scarpato R., Migliore L., Hirvonen A., Falck G., Norppa H. Cytogenetic monitoring of occupational exposure to pesticides: characterization of GSTM1, GSTT1, and NAT2 genotypes. Environ. Mol. Mutagen. 27:1996;263-269.
26. Windham G.C., Titenko-Holland N., Ososrio A.M., Gettner S., Reinish F., Haas R., Smith M. Genetic monitoring of malathion-exposed agricultural workers. Am. J. Ind. Med. 33:1998;164-174.
27. Plappert U.G., Stocker B., Fender H., Fliender T.M. Changes in the repair capacity of blood cells as a biomarker for chronic low-dose exposure to ionizing radiation. Environ. Mol. Mutagen. 30:1997;153-160.
28. Kreja L., Selig C., Nothdurft W. Assessment of DNA damage in canine peripheral blood and bone marrow after total body irradiation using the single-cell gel electrophoresis technique. Mutat. Res. 359:1996;63-70.
29. Hellman B., Vaghef H., Friis L., Edling C. Alkaline single cell gel electrophoresis of DNA fragments in biomonitoring for genotoxicity: an introductory study on healthy human volunteers. Int. Arch. Occup. Environ. Hlth. 69:1997;185-192.
30. Hellman B., Friis L., Vaghef H., Edling C. Alkaline single cell gel electrophoresis and human biomonitoring for genotoxicity: a study on subjects with residential exposure to radon. Mutat. Res. 442:1999;121-132.
31. Undeger U., Basaran N., Kars A., Guc D. Assessment of DNA damage in nurses handling antineoplastic drugs by alkaline comet assay. Mutat. Res. 439(2):1999;277-285.
32. Wojewódzka M., Kruszewski M., Iwanenko T., Collins A.R., Szumiel I. Lack of adverse effect of smoking habit on DNA strand breakage and base damage, as revealed by the alkaline comet assay. Mutat. Res. 440:1999;19-25.
33. Dich J., Zahm S.H., Hanberg A., Adami H.-O. Pesticides and cancer. Cancer Causes and Control. 8:1997;420-443.
34. Dich J., Wiklund K. Prostate cancer in pesticide applicators in Swedish agriculture. The Prostate. 34:1998;100-112.