메뉴 건너뛰기




Volumn 18, Issue 20, 2008, Pages 3148-3157

Self-assembly of protein nanoarrays on block copolymer templates

Author keywords

[No Author keywords available]

Indexed keywords

ABS RESINS; ADSORPTION; BIOMOLECULES; BLOCK COPOLYMERS; BODY FLUIDS; COPOLYMERIZATION; ESTERS; LITHOGRAPHY; NANOSTRUCTURED MATERIALS; NANOTECHNOLOGY; NUCLEIC ACIDS; ORGANIC ACIDS; PLASTIC PRODUCTS; POLYSTYRENES; PROTEINS; SELF ASSEMBLY;

EID: 55349142167     PISSN: 1616301X     EISSN: 16163028     Source Type: Journal    
DOI: 10.1002/adfm.200800487     Document Type: Article
Times cited : (62)

References (83)
  • 11
    • 84890662084 scopus 로고    scopus 로고
    • Eds: C. A. Mirkin, C. M. Niemeyer, Wiley-VCH, Weinheim
    • C. A. Mirkin, in Nanobiotechnology II, (Eds: C. A. Mirkin, C. M. Niemeyer), Wiley-VCH, Weinheim 2007, pp. 242-248.
    • (2007) Nanobiotechnology II , pp. 242-248
    • Mirkin, C.A.1
  • 34
    • 1842289819 scopus 로고    scopus 로고
    • M. Park, C. Harrison, P. M. Chaikin, R. A. Register, D. H. Adamson, Science 1997, 276, 1401.
    • M. Park, C. Harrison, P. M. Chaikin, R. A. Register, D. H. Adamson, Science 1997, 276, 1401.
  • 60
    • 55349119090 scopus 로고    scopus 로고
    • -1). Repeated scanning of an area using these tips or using cantilevers of higher spring constants induced deformation and nanoscale height differences. See also [39].
    • -1). Repeated scanning of an area using these tips or using cantilevers of higher spring constants induced deformation and nanoscale height differences. See also [39].
  • 64
    • 55349115324 scopus 로고    scopus 로고
    • The number of IgG to each cluster could not be directly counted because AFM only provides a top view and, although both BSA and IgG only form monolayers when adsorbed under our conditions [7,45,47, tip convolution also limits the spatial definition of the measured features. By considering the PS nanodomain diameter, and assuming that each IgG occupied an area equivalent to a circle with the IgG's longest axis as the diameter, we arrive at a lower bound estimate of five IgOs per nanoclusters
    • The number of IgG to each cluster could not be directly counted because AFM only provides a top view and, although both BSA and IgG only form monolayers when adsorbed under our conditions [7,45,47], tip convolution also limits the spatial definition of the measured features. By considering the PS nanodomain diameter, and assuming that each IgG occupied an area equivalent to a circle with the IgG's longest axis as the diameter, we arrive at a lower bound estimate of five IgOs per nanoclusters.
  • 73
    • 55349131950 scopus 로고    scopus 로고
    • We also measured static water contact angles of 88+/-1°, and 60.4+/-2.1°, on our PS and PMMA surfaces, respectively.
    • We also measured static water contact angles of 88+/-1°, and 60.4+/-2.1°, on our PS and PMMA surfaces, respectively.
  • 75
    • 55349107497 scopus 로고    scopus 로고
    • Although Figure 3B, D, and F on their own cannot prove that IgG had selectively adsorbed on PS, a series of samples prepared with shorter immersion times than shown in Figure 3 showed the segregation of proteins on PS domains by revealing at the same time the phase contrast pattern between PS and PMMA see Supporting Information
    • Although Figure 3B, D, and F on their own cannot prove that IgG had selectively adsorbed on PS, a series of samples prepared with shorter immersion times than shown in Figure 3 showed the segregation of proteins on PS domains by revealing at the same time the phase contrast pattern between PS and PMMA (see Supporting Information.).
  • 82
    • 55349106835 scopus 로고    scopus 로고
    • Ethylene glycol bis(succinimidyl succinate) (EGS) purchased from Pierce Biotechnology, Rockford, USA, was dissolved, at a concentration of 2mM, in a sulfoNHS-1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) amine coupling solution mixture purchased from Biacore (GE Healthcare Europe, Freiburg, Germany). The as purchased amine coupling mixture consisted of 750 mg EDC, 115 mg N-hydroxysuccinimide (NHS), 10.5 mL 1.0 M ethanolamine-HCl pH 8.5. To perform the crosslinking, the sample was immersed in 500 μL of the mixture for 90 min then rinsed in deionzed water.
    • Ethylene glycol bis(succinimidyl succinate) (EGS) purchased from Pierce Biotechnology, Rockford, USA, was dissolved, at a concentration of 2mM, in a sulfoNHS-1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) amine coupling solution mixture purchased from Biacore (GE Healthcare Europe, Freiburg, Germany). The as purchased amine coupling mixture consisted of 750 mg EDC, 115 mg N-hydroxysuccinimide (NHS), 10.5 mL 1.0 M ethanolamine-HCl pH 8.5. To perform the crosslinking, the sample was immersed in 500 μL of the mixture for 90 min then rinsed in deionzed water.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.