Quantitative analysis of isoprenoid diphosphate intermediates in recombinant and wild-type Escherichia coli strains

Applied Microbiology and Biotechnology

Volumn 81, Issue 1, 2008, Pages 175-182

  Vallon, T ^a   Ghanegaonkar, S ^a   Vielhauer, O ^a   Muller A ^a   Albermann, C ^a   Sprenger, G ^a   Reuss, M ^a   Lemuth, K ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Chromosomal integration; Geranylgeranyl diphosphate synthase; Heterologous expression; Isoprenoid diphosphate; Quantification

Indexed keywords

BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; BIOTECHNOLOGY; CHROMATOGRAPHIC ANALYSIS; ENZYMES; GAS CHROMATOGRAPHY; GENE EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; SEPARATION; WATER POLLUTION;

CHROMOSOMAL INTEGRATION; GERANYLGERANYL DIPHOSPHATE SYNTHASE; HETEROLOGOUS EXPRESSION; ISOPRENOID DIPHOSPHATE; QUANTIFICATION;

ESCHERICHIA COLI;

FARNESYL DIPHOSPHATE; FARNESYL TRANS TRANSFERASE; GERANYLGERANYL PYROPHOSPHATE;

BACTERIUM; CHROMOSOME; EXTRACTION METHOD; QUANTITATIVE ANALYSIS; RECOMBINATION; WILD POPULATION;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CONTROLLED STUDY; DEPHOSPHORYLATION; ESCHERICHIA COLI; GAS CHROMATOGRAPHY; GENE EXPRESSION; MASS SPECTROMETRY; NONHUMAN;

ALCOHOLS; BIOMASS; BIOREACTORS; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GENETIC ENGINEERING; POLYISOPRENYL PHOSPHATES;

ESCHERICHIA COLI;

EID: 54349128025     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1707-8     Document Type: Article

References (33)

1. Albermann C, Ghanegaonkar S, Lemuth K, Vallon T, Reuss M, Armbruster W, Sprenger GA (2008) Biosynthesis of the Vitamin E compound delta-tocotrienol in recombinant Escherichia coli cells. ChemBioChem (in press)
2. T Baba J Muth CM Allen 1985 Photoaffinity labeling of undecaprenyl pyrophosphate synthetase with a farnesyl pyrophosphate analogue J Biol Chem 260 10467 10473
3. P Beyer K Kreuz H Kleinig 1985 Separation of mevalonate phosphates and isopentenyl pyrophosphate by thin-layer chromatography and of short-chain prenyl phosphates by ion-pair chromatography on a high-performance liquid chromatography column Methods Enzymol 111 248 252
4. E Bruenger HC Rilling 1988 Determination of isopentenyl diphosphate and farnesyl diphosphate in tissue samples with a comment on secondary regulation of polyisoprenoid biosynthesis Anal Biochem 173 321 327
5. MC Chang JD Keasling 2006 Production of isoprenoid pharmaceuticals by engineered microbes Nat Chem Biol 2 674 681
6. R cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant Gene 158 9 14
7. Chiang CJ, Chen PT, Chao YP (2008) Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli. Biotechnol Bioeng. doi: 10.1002/bit.21976
8. A Das SH Yoon SH Lee JY Kim DK Oh SW Kim 2007 An update on microbial carotenoid production: application of recent metabolic engineering tools Appl Microbiol Biotechnol 77 505 512
9. KA Datsenko BL Wanner 2000 One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products Proc Natl Acad Sci USA 97 6640 6645
10. WR Farmer JC Liao 2000 Improving lycopene production in Escherichia coli by engineering metabolic control Nat Biotechnol 18 533 537
11. S Fujisaki T Nishino H Katsuki 1986 Isoprenoid synthesis in Escherichia coli. Separation and partial purification of four enzymes involved in the synthesis J Biochem 99 1327 1337
12. WN Hunter 2007 The non-mevalonate pathway of isoprenoid precursor biosynthesis J Biol Chem 282 21573 21577
13. KL Jones SW Kim JD Keasling 2000 Low-copy plasmids can perform as well as or better than high-copy plasmids for metabolic engineering of bacteria Metab Eng 2 328 338
14. T Kainou K Okada K Suzuki T Nakagawa H Matsuda M Kawamukai 2001 Dimer formation of octaprenyl-diphosphate synthase (IspB) is essential for chain length determination of ubiquinone J Biol Chem 276 7876 7883
15. J Kato S Fujisaki K Nakajima Y Nishimura M Sato A Nakano 1999 The Escherichia coli homologue of yeast RER2, a key enzyme of dolichol synthesis, is essential for carrier lipid formation in bacterial cell wall synthesis J Bacteriol 181 2733 2738
16. RK Keller 1996 Squalene synthase inhibition alters metabolism of nonsterols in rat liver Biochim Biophys Acta 1303 169 179
17. T Kuzuyama 2002 Mevalonate and nonmevalonate pathways for the biosynthesis of isoprene units Biosci Biotechnol Biochem 66 1619 1627
18. PC Lee BN Mijts C Schmidt-Dannert 2004 Investigation of factors influencing production of the monocyclic carotenoid torulene in metabolically engineered Escherichia coli Appl Microbiol Biotechnol 65 538 546
19. VJ Martin DJ Pitera ST Withers JD Newman JD Keasling 2003 Engineering a mevalonate pathway in Escherichia coli for production of terpenoids Nat Biotechnol 21 796 802
20. BN Mijts C Schmidt-Dannert 2003 Engineering of secondary metabolite pathways Curr Opin Biotechnol 14 597 602
21. N Misawa M Nakagawa K Kobayashi S Yamano Y Izawa K Nakamura K Harashima 1990 Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli J Bacteriol 172 6704 6712
22. DJ Pitera CJ Paddon JD Newman JD Keasling 2007 Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli Metab Eng 9 193 207
23. M Rohmer M Knani P Simonin B Sutter H Sahm 1993 Isoprenoid biosynthesis in bacteria: a novel pathway for the early steps leading to isopentenyl diphosphate Biochem J 295 Pt 2 517 524
24. M Rohmer M Seemann S Horbach S Bringer-Meyer H Sahm 1996 Glyceraldehyde 3-phosphate and pyruvate as precursors of isoprenic units in an alternative non-mevalonate pathway for terpenoid biosynthesis J Am Chem Soc 118 2564 2566
25. A Ruther N Misawa P Boger G Sandmann 1997 Production of zeaxanthin in Escherichia coli transformed with different carotenogenic plasmids Appl Microbiol Biotechnol 48 162 167
26. Y Saisho A Morimoto T Umeda 1997 Determination of farnesyl pyrophosphate in dog and human plasma by high-performance liquid chromatography with fluorescence detection Anal Biochem 252 89 95
27. L Song 2003 Detection of farnesyl diphosphate accumulation in yeast ERG9 mutants Anal Biochem 317 180 185
28. U Theobald W Mailinger M Baltes M Rizzi M Reuss 1997 In vivo analysis of metabolic dynamics in Saccharomyces cerevisiae. 1. Experimental observations Biotechnol Bioeng 55 305 316
29. H Tong SA Holstein RJ Hohl 2005 Simultaneous determination of farnesyl and geranylgeranyl pyrophosphate levels in cultured cells Anal Biochem 336 51 59
30. SH Yoon JE Kim SH Lee HM Park MS Choi JY Kim SH Lee YC Shin JD Keasling SW Kim 2007 Engineering the lycopene synthetic pathway in E. coli by comparison of the carotenoid genes of Pantoea agglomerans and Pantoea ananatis Appl Microbiol Biotechnol 74 131 139
31. SH Yoon HM Park JE Kim SH Lee MS Choi JY Kim DK Oh JD Keasling SW Kim 2007 Increased beta-carotene production in recombinant Escherichia coli harboring an engineered isoprenoid precursor pathway with mevalonate addition Biotechnol Prog 23 599 605
32. LZ Yuan PE Rouviere RA Larossa W Suh 2006 Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli Metab Eng 8 79 90
33. T Zeppenfeld C Larisch JW Lengeler K Jahreis 2000 Glucose transporter mutants of Escherichia coli K-12 with changes in substrate recognition of IICB(Glc) and induction behavior of the ptsG gene J Bacteriol 182 4443 4452