Increased β-carotene production in recombinant Escherichia coli harboring an engineered isoprenoid precursor pathway with mevalonate addition

Biotechnology Progress

Volumn 23, Issue 3, 2007, Pages 599-605

  Yoon, Sang Hwal ^a   Park, Hye Min ^a   Kim, Ju Eun ^a   Lee, Sook Hee ^a   Choi, Myung Suk ^a   Kim, Jae Yean ^a   Oh, Deok Kun ^b   Keasling, Jay D ^c   Kim, Seon Won ^a  

^a Gyeongsang National University   (South Korea)

^b Konkuk University   (South Korea)

^c University of California   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CAROTENE; ISOPENTENYL DIPHOSPHATE; MEVALONATE;

ESCHERICHIA COLI; GENES; GLYCEROL; SYNTHESIS (CHEMICAL);

PIGMENTS;

BACTERIAL PROTEIN; BETA CAROTENE; GLYCEROL; HEMITERPENE; ISOMERASE; ISOPENTENYL DIPHOSPHATE; LYCOPENE CYCLASE ISOMERASE; LYCOPENE CYCLASE-ISOMERASE; MEVALONIC ACID; ORGANOPHOSPHORUS COMPOUND; TERPENE; UNCLASSIFIED DRUG;

ARTICLE; BIOLOGICAL MODEL; DRUG EFFECT; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; PANTOEA;

BACTERIAL PROTEINS; BETA CAROTENE; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; GLYCEROL; HEMITERPENES; INTRAMOLECULAR LYASES; MEVALONIC ACID; MODELS, BIOLOGICAL; ORGANOPHOSPHORUS COMPOUNDS; PANTOEA; TERPENES;

ESCHERICHIA COLI; PANTOEA; PANTOEA AGGLOMERANS; PANTOEA ANANATIS;

EID: 34250306816     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp070012p     Document Type: Article

References (40)

1. Armstrong, G. A. Eubacteria show their true colors: Genetics of carotenoid pigment biosynthesis from microbes to plants. J. Bacteriol. 1994, 176, 4795-4802.
2. Armstrong, G. A. Genetics of eubacterial carotenoid biosynthesis: A colorful tale. Annu. Rev. Microbiol. 1997, 51, 629-659.
3. Sandmann, G. Carotenoid biosynthesis and biotechnological application. Arch. Biochem. Biophys. 2001, 385, 4-12.
4. Dufosse, L.; Galaup, P.; Yaron, A.; Arad, S. M.; Blanc, P.; Murthy, K. N. C.; Ravishankar, G. A. Microorganisms and microalgae as sources of pigments for food use: A scientific oddity or an industrial reality? Trends Food Sci. Technol. 2005, 16, 389-406.
5. Sandmann, G. Carotenoid biosynthesis in microorganisms and plants. Eur. J. Biochem. 1994, 223, 7-24.
6. Alper, H.; Miyaoku, K.; Stephanopoulos, G. Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets. Nat. Biotechnol. 2005, 23, 612-616.
7. Ruther, A.; Misawa, N.; Boger, P.; Sandmann, G. Production of zeaxanthin in Escherichia coli transformed with different carotenogenic plasmids. Appl. Microbiol. Biotechnol. 1997, 48, 162-167.
8. Wang, C. W.; Oh, M. K.; Liao, J. C. Engineered isoprenoid pathway enhances astaxanthin production in Escherichia coli. Biotechnol. Bioeng. 1999, 62, 235-241.
9. Dowling, J. E.; Wald, G. The biological function of vitamin a acid. Proc. Natl. Acad. Sci. U.S.A. 1960, 46, 587-608.
10. Napoli, J. L. Biochemical pathways of retinoid transport, metabolism, and signal transduction. Clin. Immunol. Immunopathol. 1996, 80, S52-S62.
11. Lotan, T.; Hirschberg, J. Cloning and expression in Escherichia coli of the gene encoding beta-C-4-oxygenase, that converts beta-carotene to the ketocarotenoid canthaxanthin in Haematococcus pluvialis. FEBS Lett. 1995, 364, 125-128.
12. Rohmer, M.; Knani, M.; Simonin, P.; Sutter, B.; Sahm, H. Isoprenoid biosynthesis in bacteria: A novel pathway for the early steps leading to isopentenyl diphosphate. Biochem. J. 1993, 295 (Pt 2), 517-524.
13. Boucher, Y.; Doolittle, W. F. The role of lateral gene transfer in the evolution of isoprenoid biosynthesis pathways. Mol. Microbiol. 2000, 37, 703-716.
14. Rohdich, F.; Hecht, S.; Gartner, K.; Adam, P.; Krieger, C.; Amslinger, S.; Arigoni, D.; Bacher, A.; Eisenreich, W. Studies on the nonmevalonate terpene biosynthetic pathway: metabolic role of IspH (LytB) protein. Proc. Natl. Acad. Sci. U.S.A. 2002, 99, 1158-1163.
15. Lichtenthaler, H. K.; Rohmer, M.; Schwender, J. Two independent biochemical pathways for isopentenyl diphosphate and isoprenoid biosynthesis in higher plants. Physiol. Plant 1997, 101, 643-651.
16. Hamano, Y.; Dairi, T.; Yamamoto, M.; Kuzuyama, T.; Itoh, N.; Seto, H. Growth-phase dependent expression of the mevalonate pathway in a terpenoid antibiotic-producing Streptomyces strain. Biosci. Biotechnol. Biochem. 2002, 66, 808-819.
17. Farmer, W. R.; Liao, J. C. Precursor balancing for metabolic engineering of lycopene production in Escherichia coli. Biotechnol. Prog. 2001, 17, 57-61.
18. Kajiwara, S.; Fraser, P. D.; Kondo, K.; Misawa, N. Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli. Biochem. J. 1997, 324 (Pt 2), 421-426.
19. Kim, S. W.; Keasling, J. D. Metabolic engineering of the nonmevalonate isopentenyl diphosphate synthesis pathway in Escherichia coli enhances lycopene production. Biotechnol. Bioeng. 2001, 72, 408-415.
20. Martin, V. J.; Pitera, D. J.; Withers, S. T.; Newman, J. D.; Keasling, J. D. Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Nat. Biotechnol. 2003, 21, 796-802.
21. Vadali, R. V.; Fu, Y.; Bennett, G. N.; San, K. Y. Enhanced lycopene productivity by manipulation of carbon flow to isopentenyl diphosphate in Escherichia coli. Biotechnol. Prog. 2005, 21, 1558-1561.
22. Yoon, S. H.; Lee, Y. M.; Kim, J. E.; Lee, S. H.; Lee, J. H.; Kim, J. Y.; Jung, K. H.; Shin, Y. C.; Keasling, J. D.; Kim, S. W. Enhanced lycopene production in Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate. Biotechnol. Bioeng. 2006, 94, 1025-1032.
23. Misawa, N.; Nakagawa, M.; Kobayashi, K.; Yamano, S.; Izawa, Y.; Nakamura, K.; Harashima, K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 1990, 172, 6704-6712.
24. Hundle, B.; Alberti, M.; Nievelstein, V.; Beyer, P.; Kleinig, H.; Armstrong, G. A.; Burke, D. H.; Hearst, J. E. Functional assignment of Erwinia herbicola Eho10 carotenoid genes expressed in Escherichia coli. Mol. Gen. Genet. 1994, 245, 406-416.
25. Schnurr, G.; Schmidt, A.; Sandmann, G. Mapping of a carotenogenic gene cluster from Erwinia herbicola and functional identification of six genes. FEMS Microbiol. Lett. 1991, 62, 157-161.
26. Cunningham, F. X., Jr.; Chamovitz, D.; Misawa, N.; Gantt, E.; Hirschberg, J. Cloning and functional expression in Escherichia coli of a cyanobacterial gene for lycopene cyclase, the enzyme that catalyzes the biosynthesis of beta-carotene. FEBS Lett. 1993, 328, 130-138.
27. Kang, M. J.; Yoon, S. H.; Lee, Y. M.; Lee, S. H.; Kim, J. E.; Jung, K. H.; Shin, Y. C.; Kim, S. W. Enhancing lycopene production in Escherichia coli by optimization of lycopene synthetic pathway. J. Microbiol. Biotechnol. 2005, 15, 880-886.
28. Kim, C. M.; Goldstein, J. L.; Brown, M. S. cDNA cloning of MEV, a mutant protein that facilitates cellular uptake of mevalonate, and identification of the point mutation responsible for its gain of function. J. Biol. Chem. 1992, 267, 23113-23121.
29. Sambrook, J.; Russell, D. W. Molecular Cloning, 3rd ed.; Cold Spring Harbor Laboratory Press,: New York, 2001.
30. Otsuka, K.; Itoh, M.; Yoshizawa, K. On the determination of mevalonic acid by thin-layer and gas chromatography. Agric. Biol. Chem. 1913, 37, 1013-1017.
31. Tabata, K.; Hashimoto, S. Production of mevalonate by a metabolically-engineered Escherichia coli. Biotechnol. Lett. 2004, 26, 1487-1491.
32. Yoon, S. H.; Kim, J. E.; Lee, S. H.; Park, H. M.; Choi, M. S.; Kim, J. Y.; Lee, S. H.; Shin, Y. C.; Keasling, J. D.; Kim, S. W. Engineering the lycopene synthetic pathway in E. coli by comparison of the carotenoid genes of Pantoea agglomerans and Pantoea ananatis. Appl. Microbiol. Biotechnol. 2007, 74, 131-139.
33. Lee, P. C.; Mijts, B. N.; Schmidt-Dannert, C. Investigation of factors influencing production of the monocyclic carotenoid torulene in metabolically engineered Escherichia coli. Appl. Microbiol. Biotechnol. 2004, 65, 538-546.
34. Tao, L.; Jackson, R. E.; Rouviere, P. E.; Cheng, Q. Isolation of chromosomal mutations that affect carotenoid production in Escherichia coli: Mutations alter copy number of ColE1-type plasmids. FEMS Microbiol. Lett. 2005, 243, 227-233.
35. Harker, M.; Bramley, P. M. Expression of prokaryotic 1-deoxy-D- xylulose-5-phosphatases in Escherichia coli increases carotenoid and ubiquinone biosynthesis. FEBS Lett. 1999, 448, 115-119.
36. Yuan, L. Z.; Rouviere, P. E.; Larossa, R. A.; Suh, W. Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli. Metab. Eng. 2006, 8, 79-90.
37. Lee, P. C.; Schmidt-Dannert, C. Metabolic engineering towards biotechnological production of carotenoids in microorganisms. Appl. Microbiol. Biotechnol. 2002, 60, 1-11.
38. Kim, S. W.; Kim, J. B.; Jung, W. H.; Kim, J. H.; Jung, J. K. Over-production of beta-carotene from metabolically engineered Escherichia coli. Biotechnol. Lett. 2006, 28, 897-904.
39. Alper, H.; Miyaoku, K.; Stephanopoulos, G. Characterization of lycopene-overproducing E. coli strains in high cell density fermentations. Appl. Microbiol. Biotechnol. 2006, DOI 10.1007/s00253-006-0357-y.
40. Cheng, Q. Structural diversity and functional novelty of new carotenoid biosynthesis genes. J. Ind. Microbiol. Biotechnol. 2006, 33, 552-559.