Replicon-free and markerless methods for genomic insertion of DNAs in phage attachment sites and controlled expression of chromosomal genes in Escherichia coli

Biotechnology and Bioengineering

Volumn 101, Issue 5, 2008, Pages 985-995

  Chiang, Chung Jen ^a   Po, Ting Chen ^b   Chao, Yun Peng ^c  

^a CHINA MEDICAL UNIVERSITY   (Taiwan)

^b ACADEMIA SINICA   (Taiwan)

^c FENG CHIA UNIVERSITY   (Taiwan)

Author keywords

Genome engineering; Lycopene production; Metabolic engineering; Phage attachment site

Indexed keywords

ABS RESINS; ESCHERICHIA COLI; NUCLEIC ACIDS; ORGANIC ACIDS; PHOTOLITHOGRAPHY; RNA; VECTORS; WATER POLLUTION;

ATTACHMENT SITES; E. COLI; GENETIC MANIPULATIONS; GENOME ENGINEERING; GENOME ENGINEERINGS; GENOMIC GENES; HETEROLOGOUS GENES; INTEGRATION VECTORS; LYCOPENE; LYCOPENE PRODUCTION; MARKERLESS; METABOLIC ENGINEERING; PHAGE ATTACHMENT SITE; PLASMID INSTABILITIES; POTENTIAL PROBLEMS; PROPHAGE; RESPONSE REGULATORS; RNA POLYMERASES; STABLE PRODUCTIONS; TARGET GENES; TEMPLATE VECTORS;

GENES;

BACTERIAL DNA; LYCOPENE; RECOMBINASE; RNA POLYMERASE;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL GENOME; BACTERIOPHAGE; BIOENGINEERING; CRTB GENE; CRTI GENE; DXS GENE; ESCHERICHIA COLI; GENE CASSETTE; GENE EXPRESSION REGULATION; GENE FUSION; GENE INSERTION; GENE TARGETING; GENETIC ENGINEERING; GENETIC MANIPULATION; GPS GENE; MARKER GENE; NONHUMAN; PCKA GENE; PLASMID; PROMOTER REGION; PROPHAGE; REGULON; REPLICON;

ATTACHMENT SITES, MICROBIOLOGICAL; CAROTENOIDS; CHROMOSOMES; CLONING, MOLECULAR; DNA; DNA NUCLEOTIDYLTRANSFERASES; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; FARNESYL-DIPHOSPHATE FARNESYLTRANSFERASE; FARNESYLTRANSTRANSFERASE; GENE EXPRESSION REGULATION, BACTERIAL; GENE TARGETING; GENES, REGULATOR; GENETIC ENHANCEMENT; GENETIC MARKERS; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; OXIDOREDUCTASES; PHOSPHOENOLPYRUVATE CARBOXYKINASE (ATP); PLASMIDS; RECOMBINATION, GENETIC; REPLICON; TRANSFERASES; VIRAL PROTEINS;

ESCHERICHIA COLI;

EID: 54349126905     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21976     Document Type: Article

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