Controlled growth of Chinese hamster ovary cells and high expression of antibody-IL-2 fusion proteins by temperature manipulation

Biotechnology Letters

Volumn 27, Issue 23-24, 2005, Pages 1879-1884

  Shi, Ming ^a   Xie, Zhigang ^a   Yu, Ming ^a   Shen, Beifen ^a   Guo, Ning ^a  

^a INSTITUTE OF BASIC MEDICAL SCIENCES   (China)

Author keywords

Antibody; Cell cycle; Fusion protein; Gene expression; Interleukin 2; Low temperature

Indexed keywords

ANTIBODIES; CELL CULTURE; GROWTH KINETICS;

CELL CYCLE; FUSION PROTEINS; GENE EXPRESSION; INTERLEUKIN 2; LOW TEMPERATURE;

PROTEINS;

ANTIBODY; DIHYDROFOLATE REDUCTASE; EPIDERMAL GROWTH FACTOR RECEPTOR 2; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN FRAGMENT; IMMUNOGLOBULIN G; INTERLEUKIN 2; METHOTREXATE;

ANIMAL; APOPTOSIS; ARTICLE; BIOSYNTHESIS; CELL CYCLE; CELL PROLIFERATION; CELL SURVIVAL; CHO CELL; CRICETULUS; DRUG EFFECT; FLOW CYTOMETRY; GENE AMPLIFICATION; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; PHYSIOLOGY; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; TEMPERATURE;

ANIMALS; ANTIBODIES; APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; CRICETINAE; CRICETULUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLOW CYTOMETRY; GENE AMPLIFICATION; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN G; IMMUNOGLOBULIN VARIABLE REGION; INTERLEUKIN-2; METHOTREXATE; PLASMIDS; RECEPTOR, ERBB-2; RECOMBINANT FUSION PROTEINS; TEMPERATURE; TETRAHYDROFOLATE DEHYDROGENASE; TRANSFECTION;

CRICETULUS GRISEUS;

EID: 28444432735     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-005-3897-9     Document Type: Article

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