Pilot production of u-PA with porous microcarrier cell culture

Cytotechnology

Volumn 33, Issue 1-3, 2000, Pages 13-19

  Hu, Xianwen ^a   Xiao, Chengzu ^a   Huang, Zicai ^a   Guo, Zhixia ^a   Zhang, Zhengguang ^a   Li, Zuohu ^b  

^a Beijing Institute of Cell Biotechnology   (China)

^b INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

Cell culture; Porous microcarrier; Prourokinase

Indexed keywords

CELL ACTIVATION; CELL CULTURE; CELL DENSITY; CELL LINE; CELLULOSE; CHO CELL; CULTURE MEDIUM; ENZYME ACTIVITY; PLASMINOGEN; PROCESS OPTIMIZATION; STIRRED REACTOR; TEMPERATURE; UROKINASE;

BOVINAE; VISCUM ALBUM;

EID: 0033791647     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/a:1008127310890     Document Type: Conference Paper

References (14)

1. Cell death (apoptosis) in cell culture systems
2. Immobilization of animal cells in porous carriers culture
3. The construction and expression of human prourokinase cDNA coding deleted mutant at 150~156 amino acids
4. Large scale culture of rCHO cells using porous microcarriers
5. Long-term culture of CHO cells on porous microcarriers in a stirred tank equipped with a modified cell retention system
6. Production of u-PA with rCHO cell culture on porous microcarriers in serum-free growth medium
7. The activation of prourokinase by plasma kallikrein and its inactivation by thrombin
8. Subculture method for large scale cell culture using macroporous microcarrier
9. Clinical studies with the new glycosylated recombinant prourokinase
10. High density culture using macroporous microcarrier
11. An autocontroller system for cell perfusion cultivation on microcarrier
12. High density cultivation of genetically-engineered CHO cell lines with microcarrier culture systems
13. High density cultivation of recombinant CHO cell line and two strains of hybridomas with porous microcarrier Cytopore
14. High density and scale-up cultivation of recombinant CHO cell line and hybridomas with porous microcarrier Cytopore