High cell density and productivity culture of Chinese hamster ovary cells in a fluidized bed bioreactor

Cytotechnology

Volumn 29, Issue 3, 1999, Pages 215-220

  Kong, D ^a   Cardak, S ^a   Chen, M ^a   Gentz, R ^a   Zhang, Junli ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

CHO; Cytoline microcarrier; Fluidized bed bioreactor (FBR); Stirred tank bioreactor (STR)

Indexed keywords

CELL CULTURE; CHO CELL; CYTODEX 1; CYTOLINE 1; FLUIDIZED BED REACTOR;

EID: 0032892998     PISSN: 09209069     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008064217040     Document Type: Article

References (10)

1. Griffiths JB, Looby D and Racher AJ (1992) Maximisation of perfusion systems and process comparison with batch- type cultures. Maximisation of perfusion cultures. Cytotechnology 9(1-3): 3-9.
2. Kratje RB, Reimann A, Hammer J and Wagner R (1994) Cultivation of recombinant baby hamster kidney cells in a fluidized bed bioreactor system with porous borosilicate glass. Biotechnol Prog 10(4): 410-420.
3. Lazar A, Reuveny S, Kronman C, Velan B and Shafferman A (1993) Evaluation of anchorage-dependent cell propagation systems for production of human acetylcholinesterase by recombinant 293 cells. Cytotechnology 13(2): 115-123.
4. Mignot G, Faure T, Ganne, Arbeille B, Pavirani A and Romet-Lemonne JL (1990) Production of recombinant Von Willebrand factor by CHO cells cultured in macroporous microcarriers. Cytotechnology 4(2): 163-171.
5. Nikolai TJ and Hu WS (1992) Cultivation of mammalian cells on macroporous microcarriers. Enzyme Microb Technol 14(3): 203-208.
6. Reiter M, Bluml G, Gaida T, Zach N, Unterluggauer F, Doblhoff-Dier O, Noe M, Plail R, Huss S and Katinger H (1991) Modular integrated fluidized bed bioreactor technology. Bio/Technology 9: 1100-1102.
7. Reiter M, Hohenwarter O, Gaida T, Zach N, Schmatz C, Bluml G, Weigang F, Nilsson K and Katinger H (1990) The use of macroporous gelatin carriers for the cultivation of mammalian cells in fluidised bed reactors. Cytotechnology 3(3): 271-277.
8. Reiter M, Weigang F, Ernst W and Katinger HWD (1990) High density microcarrier culture with a new device which allows oxygenation and perfusion of microcarrier cultures. Cytotechnology 3(1): 39-42.
9. Smits P, Ni J, Feng P, Wauters J, Van Hul W, Boutaibi ME, Dillon PJ and Merregaert J (1997) The human extracellular matrix gene 1 (ECM1): genomic structure, cDNA cloning, expression pattern, and chromosomal localization. Genomics 45(3): 487-495.
10. Vournakis JN and Runstadler PW, Jr. (1991) Optimization of the microenvironment for mammalian cell culture in flexible collagen microspheres in a fluidized-bed bioreactor. Bio/Technology 17: 305-326.