Role of methoxypolyethylene glycol on the hydration, activity, conformation and dynamic properties of a lipase in a dry film

Biotechnology and Bioengineering

Volumn 101, Issue 2, 2008, Pages 255-262

  Secundo, Francesco ^a   Barletta, Gabriel ^b   Mazzola, Giorgio ^c  

^a CNR   (Italy)

^b UNIVERSITY OF PUERTO RICO   (Puerto Rico)

^c Jasco Europe Srl ^*  (Italy)

Author keywords

Fluorescence anisotropy; Hydrogen bonds; Infrared spectroscopy; Protein dehydration; Secondary structure; Tertiary structure

Indexed keywords

ADDITIVES; BIOTECHNOLOGY; CONFORMATIONS; CURING; DOPING (ADDITIVES); ENZYME ACTIVITY; ENZYMES; ETHYLENE GLYCOL; FLUORESCENCE; GLYCOLS; HYDRATION; LIGHT EMISSION; LUMINESCENCE; ORGANIC COMPOUNDS; ORGANIC SOLVENTS; PROTEINS; SPECTROSCOPIC ANALYSIS; WATER CONTENT;

FLUORESCENCE ANISOTROPY; HYDROGEN BONDS; PROTEIN DEHYDRATION; SECONDARY STRUCTURE; TERTIARY STRUCTURE;

INFRARED SPECTROSCOPY;

MACROGOL; METHOXYPOLYETHYLENE GLYCOL; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; MACROGOL DERIVATIVE; MONOMETHOXYPOLYETHYLENE GLYCOL; SOLVENT; WATER;

ARTICLE; BURKHOLDERIA CEPACIA; CATALYSIS; CONFORMATIONAL TRANSITION; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME KINETICS; FILM; FLUORESCENCE SPECTROSCOPY; HYDRATION; HYDROGEN BOND; INFRARED SPECTROSCOPY; NONHUMAN; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; CHEMISTRY; DESICCATION; ENZYMOLOGY; FLUORESCENCE POLARIZATION; KINETICS; PROTEIN CONFORMATION;

BURKHOLDERIA CEPACIA;

BACTERIAL PROTEINS; BURKHOLDERIA CEPACIA; CATALYSIS; DESICCATION; FLUORESCENCE POLARIZATION; KINETICS; LIPASE; POLYETHYLENE GLYCOLS; PROTEIN CONFORMATION; SOLVENTS; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; WATER;

EID: 51649101649     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.21888     Document Type: Article

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