Enzyme microarrays assembled by acoustic dispensing technology

Analytical Biochemistry

Volumn 381, Issue 1, 2008, Pages 101-106

  Wong, E Y ^a   Diamond, S L ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Acoustic dispense; Kinase; Microarray; Phosphocellulose paper; Phosphor storage imaging; Radioactive phosphorus; Screening assay

Indexed keywords

BIOASSAY; COST EFFECTIVENESS; LIGHT EMISSION; MICROARRAYS; PHOSPHORS; RUTHENIUM COMPOUNDS;

COEFFICIENTS OF VARIATIONS; CYCLOPENTADIENYL RUTHENIUMS; DISPENSING TECHNOLOGY; HIGH THROUGHPUT SCREENING; KINASE; PHOSPHORYLATED SUBSTRATES; SCREENING ASSAYS; SIGNAL-TO-BACKGROUND RATIO;

ENZYMES;

BUFFER; CELLULOSE PHOSPHATE; GLYCEROL; PEPTIDE; PROTEIN KINASE PIM 1; RUTHENIUM;

ARTICLE; BIOASSAY; ENZYME ASSAY; ENZYME PHOSPHORYLATION; HIGH THROUGHPUT SCREENING; INCUBATION TIME; MICROARRAY ANALYSIS; PRIORITY JOURNAL; PROTEIN DETERMINATION;

EID: 49049094185     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.06.024     Document Type: Article

References (24)

1. Vieth M., et al. Kinomics: characterizing the therapeutically validated kinase space. Drug Discov. Today 10 (2005) 839-846
2. Beasley J.R., et al. Miniaturized, ultra-high throughput screening of tyrosine kinases using homogeneous, competitive fluorescence immunoassays. Assay Drug Dev. Technol. 2 (2004) 141-151
3. Kupcho K., et al. A homogeneous, nonradioactive high-throughput fluorogenic protein kinase assay. Anal. Biochem. 317 (2003) 210-217
4. Tagliati F., et al. Utilization of luminescent technology to develop a kinase assay: Cdk4 as a model system. J. Pharm. Biomed. Anal. 39 (2005) 811-814
5. Lesaicherre M.L., et al. Antibody-based fluorescence detection of kinase activity on a peptide array. Bioorg. Med. Chem. Lett. 12 (2002) 2085-2088
6. Asensio C.J., and Garcia R.C. Determination of a large number of kinase activities using peptide substrates, P81 phosphocellulose paper arrays and phosphor imaging. Anal. Biochem. 319 (2003) 21-33
7. Hodder P., et al. Miniaturization of intracellular calcium functional assays to 1536-well plate format using a fluorometric imaging plate reader. J. Biomol. Screen. 9 (2004) 417-426
8. Lavery P., Brown M.J., and Pope A.J. Simple absorbance-based assays for ultra-high throughput screening. J. Biomol. Screen. 6 (2001) 3-9
9. Wolcke J., and Ullmann D. Miniaturized HTS technologies: uHTS. Drug Discov. Today 6 (2001) 637-646
10. Horiuchi K.Y., et al. Microarrays for the functional analysis of the chemical-kinase interactome. J. Biomol. Screen. 11 (2006) 48-56
11. MacBeath G. Protein microarrays and proteomics. Nat. Genet. 32 Suppl. (2002) 526-532
12. MacBeath G., and Schreiber S.L. Printing proteins as microarrays for high-throughput function determination. Science 289 (2000) 1760-1763
13. Gosalia D.N., et al. Profiling serine protease substrate specificity with solution phase fluorogenic peptide microarrays. Proteomics 5 (2005) 1292-1298
14. Horiuchi K.Y., et al. Microarrays for the functional analysis of the chemical-kinase interactome. J Biomol Screen 11 1 (2006) 48-56
15. Pease A.C., et al. Light-generated oligonucleotide arrays for rapid DNA sequence analysis. Proc. Natl. Acad. Sci. USA 91 (1994) 5022-5026
16. Situma C., Hashimoto M., and Soper S.A. Merging microfluidics with microarray-based bioassays. Biomol. Eng. 23 (2006) 213-231
17. Gosalia D.N., and Diamond S.L. Printing chemical libraries on microarrays for fluid phase nanoliter reactions. Proc. Natl. Acad. Sci. USA 100 (2003) 8721-8726
18. Bachmann M., and Moroy T. The serine/threonine kinase Pim1. Int. J. Biochem. Cell Biol. 37 (2005) 726-730
19. Bregman H., Carroll P.J., and Meggers E. Rapid access to unexplored chemical space by ligand scanning around a ruthenium center: discovery of potent and selective protein kinase inhibitors. J. Am. Chem. Soc. 128 (2006) 877-884
20. J.B. Segur, in: Physical Properties of Glycerol and Its Solutions, Glycerine Producers' Assoc., New York, 1963, p. 269.
21. Zhang L., Carroll P., and Meggers E. Ruthenium complexes as protein kinase inhibitors. Org. Lett. 6 (2004) 521-523
22. Bregman H., and Meggers E. Ruthenium half-sandwich complexes as protein kinase inhibitors: an N-succinimidyl ester for rapid derivatizations of the cyclopentadienyl moiety. Org. Lett. 8 (2006) 5465-5468
23. Myers M.C., et al. Identification and synthesis of a unique thiocarbazate cathepsin L inhibitor. Bioorg. Med. Chem. Lett. 18 (2008) 210-214
24. Zhang J.H., Chung T.D., et al. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4 (1999) 67-73