A homogeneous, nonradioactive high-throughput fluorogenic protein kinase assay

Analytical Biochemistry

Volumn 317, Issue 2, 2003, Pages 210-217

  Kupcho, Kevin ^a   Somberg, Richard ^a   Bulleit, Bob ^a   Goueli, Said A ^a,b  

^a PROMEGA CORPORATION   (United States)

^b UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

Author keywords

Enzyme assay; High throughput screening; Protein kinases; Protein phosphorylation

Indexed keywords

AMIDES; AMINO ACIDS; COST EFFECTIVENESS; DRUG PRODUCTS; ENZYME ACTIVITY; PEPTIDES; PHOSPHORYLATION;

CELLULAR RESPONSE; ENZYME ASSAYS; EXTRACELLULAR STIMULI; HIGH THROUGHPUT SCREENING; LOW CONCENTRATIONS; PEPTIDE SUBSTRATES; PROTEIN KINASE; PROTEIN PHOSPHORYLATION;

SIGNAL TO NOISE RATIO;

AMIDE; PROTEIN KINASE; PROTEIN SERINE KINASE; PROTEIN TYROSINE KINASE; RHODAMINE 110; THREONINE;

ANALYTIC METHOD; ARTICLE; CONCENTRATION (PARAMETERS); CONJUGATION; DRUG SCREENING; ENZYME ACTIVITY; MEASUREMENT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN PHOSPHORYLATION; PROTEIN TARGETING; ROBOTICS; SIGNAL NOISE RATIO; SIGNAL PROCESSING; SYSTEM ANALYSIS; TIME;

EID: 0037620455     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(03)00094-0     Document Type: Article

References (19)

1. Cohen P. The role of protein phosphorylation in human health and disease. Eur. J. Biochem. 268:2001;5001-5010.
2. Cohen P. Protein kinases - The major drug targets of the twenty-first century. Nat. Rev. Drug Discovery. 1:2002;309-315.
3. Trauger J.W., Lin F.-F., Turner M.S., Stephens J., LoGrasso P.V. Kinetic mechanism for human Rho-kinase II (ROCK-II). Biochemistry. 41:2002;8948-8953.
4. Goueli B.S., Hsiao K., Goueli S.A. Assaying activity of individual protein kinases in crude tissue or cellular extracts. Methods Enzymol. 333:2001;16-27.
5. Toomik R., Kman P., Engstrom L. A potential pitfall in protein kinase assay: phosphocellulose paper as an unreliable adsorbent of produced phosphopeptides. Anal. Biochem. 204:1992;311-314.
6. Cook N.D. Scintillation proximity assay - A versatile high throughput screening technology. Drug Discovery Today. 1:1996;287-294.
7. Braunwalder A.F., Yarwood D.R., Hall T., Missbach M., Lipson K.E., Sills M.A. A solid-phase assay for the determination of protein tyrosine kinase activity of the c-src using scintillating microtitration plates. Anal. Biochem. 234:1996;23-26.
8. Kolb A.J., Kaplita P.V., Hayes D.J., Park Y.-W., Pernell C., Major J.S., Mathis G. Tyrosine kinase assays adapted to homogenous time-resolved fluorescence. Drug Discovery Today. 3:1998;333-342.
9. Braunwalder A.F., Yarwood D.R., Sills M.A., Lipson K.E. Measurement of the protein tyrosine kinase activity of c-src using time-resolved fluorimetry of europium chelates. Anal. Biochem. 238:1996;159-164.
10. Seethala R., Menzel R. A fluorescence polarization competition immunoassay for tyrosine kinases. Anal. Biochem. 255:1998;257-262.
11. Turek T.C., Small E.C., Bryant R.W., Hill W.A.G. Development and validation of a competitive AKT serine/threonine kinase fluorescence polarization assay using a product-specific anti-phospho-serine antibody. Anal. Biochem. 299:2001;45-53.
12. Cohen, Chin-Dixon E., Jeong S., Nikiforov T.T. A microchip-based enzyme assay for protein kinase A. Anal. Biochem. 273:1999;89-97.
13. Murray P.F., Hammerschmidt P., Samela A., Passeron S. Peptide degradation: effect of substrate phosphorylation on aminopeptidasic hydrolysis. Int. J. Biochem. Cell Biol. 28:1996;451-456.
14. Dass C., Mahalakshmi P. Phosphorylation of enkephalins enhances their proteolytic stability. Life Sci. 58:1996;1039-1045.
15. Leytus S.P., Patterson W.L., Mangel W.F. New class of sensitive and selective fluorogenic substrates for serine proteinases. Amino acid and dipeptide derivatives of rhodamine. Biochem. J. 215:1983;253-260.
16. Yonemoto W.M., McGlone M.L., Slice L.W., Taylor S.S. Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase. Methods Enzymol. 200:1991;581-596.
17. Kemp B.E., Pearson R.B. Design and use of peptide substrates for protein kinases. Methods Enzymol. 200:1991;121-134.
18. Zhang J.-H., Chung T.D.Y., Oldenburg K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screening. 4:1999;67-73.
19. Tamaoki T. Use and specificity of staurosporine, UCN-01, and calphostin C as protein kinase inhibitors. Methods Enzymol. 201:1991;340-347.