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We did not observe any changes in chemical shifts over the 10-0.1 mM concentration range. This rules out the possibility of a fast monomer-dimer exchange as the cause of a single set of resonances
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We did not observe any changes in chemical shifts over the 10-0.1 mM concentration range. This rules out the possibility of a fast monomer-dimer exchange as the cause of a single set of resonances.
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Interestingly, examination of the X-ray crystal structures of 1 and 2 shows that the two internal amide protons remain free in the dimeric aggregate. Upon careful inspection, we conclude that these amide protons are actually involved in weak electrostatic interactions with the ureidyl C=O group and also that they are not accessible to any external H-bond acceptors because they are buried inside the molecular architecture.
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Interestingly, examination of the X-ray crystal structures of 1 and 2 shows that the two internal amide protons remain free in the dimeric aggregate. Upon careful inspection, we conclude that these amide protons are actually involved in weak electrostatic interactions with the ureidyl C=O group and also that they are not accessible to any external H-bond acceptors because they are buried inside the molecular architecture.
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