Overexpression of the replication licensing regulators hCdt1 and hCdc6 characterizes a subset of non-small-cell lung carcinomas: Synergistic effect with mutant p53 on tumor growth and chromosomal instability - Evidence of E2F-1 transcriptional control over hCdt1

American Journal of Pathology

Volumn 165, Issue 4, 2004, Pages 1351-1365

  Karakaidos, Panagiotis ^a   Taraviras, Stavros ^b   Vassiliou, Leandros V ^a   Zacharatos, Panayotis ^a   Kastrinakis, Nikolaos G ^a   Kougiou, Dionysia ^b   Kouloukoussa, Mirsini ^a   Nishitani, Hideo ^c   Papavassiliou, Athanasios G ^b   Lygerou, Zoi ^b   Gorgoulis, Vassilis G ^a,d  

^a UNIVERSITY OF ATHENS MEDICAL SCHOOL   (Greece)

^b UNIVERSITY OF PATRAS   (Greece)

^c KYUSHU UNIVERSITY   (Japan)

^d NONE   (Greece)

Author keywords

[No Author keywords available]

Indexed keywords

GEMININ; PROTEIN HCDC6; PROTEIN HCDT1; PROTEIN P53; REGULATOR PROTEIN; TRANSCRIPTION FACTOR E2F1; UNCLASSIFIED DRUG;

ARTICLE; CLINICAL ARTICLE; GENE OVEREXPRESSION; HUMAN; HUMAN TISSUE; LUNG CARCINOGENESIS; LUNG NON SMALL CELL CANCER; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 4644292460     PISSN: 00029440     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0002-9440(10)63393-7     Document Type: Article

References (55)

1. Paulovich AG, Toczyski DP, Hartwell LH: When checkpoints fail. Cell 1997, 88:315-321
2. Lengauer C, Kinzler KW, Vogelstein B: Genetic instabilities in human cancers. Nature 1998, 396:643-649
3. Nyberg KA, Michelson RJ, Putnam CW, Weinert TA: Toward maintaining the genome: DNA damage and replication checkpoints. Annu Rev Genet 2002, 36:617-656
4. Shreeram S, Blow JJ: The role of the replication licensing system in cell proliferation and cancer. Prog Cell Cycle Res 2003, 5:287-293
5. Blow JJ, Laskey RA: A role for the nuclear envelope in controlling DNA replication within the cell cycle. Nature 1988, 332:546-548
6. Diffley JF: DNA replication: building the perfect switch. Curr Biol 2001, 11:R367-R370
7. Lei M, Tye BK: Initiating DNA synthesis: from recruiting to activating the MCM complex. J Cell Sci 2001, 114:1447-1454
8. Bell SP, Dutta A: DNA replication in eukaryotic cells. Annu Rev Biochem 2002, 71:333-374
9. Nishitani H, Lygerou Z: Control of DNA replication licensing in a cell cycle. Genes Cells 2002, 7:523-534
10. Cocker JH, Piatti S, Santocanale C, Nasmyth K, Diffley JF: An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast. Nature 1996, 379:180-182
11. Coleman TR, Carpenter PB, Dunphy WG: The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts. Cell 1996, 87:53-63
12. Nishitani H, Lygerou Z, Nishimoto T, Nurse P: The Cdt1 protein is required to license DNA for replication in fission yeast. Nature 2000, 404:625-628
13. Maiorano D, Moreau J, Mechali M: XCDT1 is required for the assembly of pre-replicative complexes in Xenopus laevis. Nature 2000, 404:622-625
14. Tanaka T, Knapp D, Nasmyth K: Loading of an Mcm protein onto DNA replication origins is regulated by Cdc6p and CDKs. Cell 1997, 90:649-660
15. Nishitani H, Nurse P: p65cdc18 plays a major role controlling the initiation of DNA replication in fission yeast. Cell 1995, 83:397-405
16. Muzi Falconi M, Brown GW, Kelly TJ: cdc18+ regulates initiation of DNA replication in Schizosaccharomyces pombe. Proc Natl Acad Sci USA 1996, 93:1566-1570
17. Yanow SK, Lygerou Z, Nurse P: Expression of Cdc 18/Cdc6 and Cdt1 during G2 phase induces initiation of DNA replication. EMBO J 2001, 20:4648-4656
18. Vaziri C, Saxena S, Jeon Y, Lee C, Murata K, Machida Y, Wagie N, Hwang DS, Dutta A: A p53-dependent checkpoint pathway prevents rereplication. Mol Cell 2003, 11:997-1008
19. Arentson E, Faloon P, Seo J, Moon E, Studts JM, Fremont DH, Choi K: Oncogenic potential of the DNA replication licensing protein CDT1. Oncogene 2002, 21:1150-1158
20. Williams RS, Shohet RV, Stillman B: A human protein related to yeast Cdc6p. Proc Natl Acad Sci USA 1997, 94:142-147
21. Saha P, Chen J, Thome KC, Lawlis SJ, Hou ZH, Hendricks M, Parvin JD, Dutta A: Human CDC6/Cdc18 associates with Orc1 and cyclin-cdk and is selectively eliminated from the nucleus at the onset of S phase. Mol Cell Biol 1998, 18:2758-2767
22. Fujita M, Yamada C, Goto H, Yokoyama N, Kuzushima K, Inagaki M, Tsurumi T: Cell cycle regulation of human CDC6 protein. Intracellular localization, interaction with the human mcm complex, and CDC2 kinase-mediated hyperphosphorylation. J Biol Chem 1999, 274:25927-25932
23. Petersen BO, Lukas J, Sorensen CS, Bartek J, Helin K: Phosphorylation of mammalian CDC6 by cyclin A/CDK2 regulates its subcellular localization. EMBO J 1999, 18:396-410
24. Coverley D, Pelizon C, Trewick S, Laskey RA: Chromatin-bound Cdc6 persists in S and G2 phases in human cells, while soluble Cdc6 is destroyed in a cyclin A-cdk2 dependent process. J Cell Sci 2000, 113:1929-1938
25. Petersen BO, Wagener C, Marinoni F, Kramer ER, Melixetian M, Denchi EL, Gieffers C, Matteucci C, Peters JM, Helin K: Cell cycle- and cell growth-regulated proteolysis of mammalian CDC6 is dependent on APC-CDH1. Genes Dev 2000, 14:2330-2343
26. Wohlschlegel JA, Dwyer BT, Dhar SK, Cvetic C, Walter JC, Dutta A: Inhibition of eukaryotic DNA replication by geminin binding to Cdt1 Science 2000, 290:2309-2312
27. Nishitani H, Taraviras S, Lygerou Z, Nishimoto T: The human licensing factor for DNA replication Cdt1 accumulates in G1 and is destabilized after initiation of S-phase. J Biol Chem 2001, 276:44905-44911
28. Li X, Zhao Q, Liao R, Sun P, Wu X: The SCF(Skp2) ubiquitin ligase complex interacts with the human replication licensing factor Cdt1 and regulates Cdt1 degradation. J Biol Chem 2003, 278:30854-30858
29. McGarry TJ, Kirschner MW: Geminin, an inhibitor of DNA replication, is degraded during mitosis. Cell 1998, 93:1043-1053
30. Tada S, Li A, Maiorano D, Mechali M, Blow JJ: Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin. Nat Cell Biol 2001, 3:107-113
31. Gorgoulis VG, Zacharatos P, Kotsinas A, Lilogiou T, Kyroudi A, Veslemes M, Rassidakis A, Halazonetis TD, Field JK, Kittas C: Alterations of the p16-pRb pathway and the chromosome locus 9p21-22 in non-small-cell lung carcinomas: relationship with p53 and MDM2 protein expression. Am J Pathol 1998, 153:1749-1765
32. Gorgoulis VG, Zacharatos P, Kotsinas A, Mariatos G, Lilogiou T, Vogiatzi T, Foukas P, Rassidakis G, Garinis G, Ioannides T, Zoumpourlis V, Bramis J, Michail PO, Asimacopouios PJ, Field JK, Kittas C: Altered expression of the cell cycle regulatory molecules pRb, p53 and MDM2 exert a synergetic effect on tumor growth and chromosomal instability in non-small cell lung carcinomas (NSCLCs). Mol Med 2000, 6:208-237
33. Mariatos G, Gorgoulis VG, Zacharatos P, Kotsinas A, Vogiatzi T, Rassidakis G, Foukas P, Liloglou T, Tiniakos D, Angelou N, Manolis EN, Veslemes M, Field JK, Kittas C: Expression of p16(INK4A) and alterations of the 9p21-23 chromosome region in non-small-cell lung carcinomas: relationship with tumor growth parameters and ploidy status. Int J Cancer 2000, 89:133-141
34. Tsoli E, Gorgoulis VG, Zacharatos P, Kotsinas A, Mariatos G, Kastrinakis NG, Kokotas S, Kanavaros P, Asimacopoulos P, Bramis J, Kletsas D, Papavassiliou AG, Kittas C: Low levels of p27 in association with deregulated p53-pRb protein status enhance tumor proliferation and chromosomal instability in non-small cell lung carcinomas. Mol Med 2001, 7:418-429
35. Gorgoulis VG, Zacharatos P, Mariatos G, Kotsinas A, Bouda M, Kletsas D, Asimacopoulos PJ, Agnantis N, Kittas C, Papavassiliou AG: Transcription factor E2F-1 acts as a growth-promoting factor and is associated with adverse prognosis in non-small cell lung carcinomas. J Pathol 2002, 198:142-156
36. Kotsinas A, Evangelou K, Zacharatos P, Kittas C, Gorgoulis VG: Proliferation, but not apoptosis, is associated with distinct beta-catenin expression patterns in non-small-cell lung carcinomas: relationship with adenomatous polyposis coli and G(1)-to S-phase cell-cycle regulators. Am J Pathol 2002, 161:1619-1634
37. Stevaux O, Dyson NJ: A revised picture of the E2F transcriptional network and RB function. Curr Opin Cell Biol 2002, 14:684-691
38. Bermejo R, Vilaboa N, Cales C: Regulation of CDC6, geminin, and CDT1 in human cells that undergo polyploidization. Mol Biol Cell 2002, 13:3989-4000
39. Taraviras S, Schutz G, Kelsey G: Generation of inhibitory mutants of hepatocyte nuclear factor 4. Eur J Biochem 1997, 244:883-889
40. Mundle SD, Saberwal G: Evolving intricacies and implications of E2F-1 regulation. EMBO J 2003, 17:569-574
41. Van Z, DeGregori J, Shohet R, Leone G, Stillman B, Nevins JR, Williams RS: Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells. Proc Natl Acad Sci USA 1998, 95:3603-3608
42. Hateboer G, Wobst A, Petersen BO, Le Cam L, Vigo E, Sardet C, Helin K: Cell cycle-regulated expression of mammalian CDC6 is dependent on E2F. Mol Cell Biol 1998, 18:6679-6697
43. Greenblatt MS, Bennett WP, Hollstein M, Harris CC: Mutations in the p53 tumor suppressor gene: clues to cancer etiology and molecular pathogenesis. Cancer Res 1994, 54:4855-4878
44. Mihaylov IS, Kondo T, Jones L, Ryzhikov S, Tanaka J, Zheng J, Higa LA, Minamino N, Cooley L, Zhang H: Control of DNA replication and chromosome ploidy by geminin and cyclin A. Mol Cell Biol 2002, 22:1868-1880
45. Williams GH, Romanowski P, Morris L, Madine M, Mills AD, Stoeber K, Marr J, Laskey RA, Coleman N: Improved cervical smear assessment using antibodies against proteins that regulate DNA replication. Proc Natl Acad Sci USA 1998, 95:14932-14937
46. Bonds L, Baker P, Gup C, Shroyer KR: Immunohistochemical localization of cdc6 in squamous and glandular neoplasia of the uterine cervix. Arch Pathol Lab Med 2002, 26:1164-1168
47. Robles LD, Frost AR, Davila M, Hutson AD, Grizzle WE, Chakrabarti R: Down-regulation of Cdc6, a cell cycle regulatory gene, in prostate cancer. J Biol Chem 2002, 277:25431-25438
48. Yoshida K, Inoue I: Regulation of Geminin and Cdt1 expression by E2F transcription factors. Oncogene 2004, 23:3802-3812
49. Whittaker AJ, Royzman I, Orr-Weaver TL: Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts. Genes Dev 2000, 14:1765-1776
50. Humbert PO, Verona R, Trimarchi JM, Rogers C, Dandapani S, Lees JA: E2f3 is critical for normal cellular proliferation. Genes Dev 2000, 14:690-703
51. Schlisio S, Halperin T, Vidal M, Nevins JR: Interaction of YY1 with E2Fs, mediated by RYBP, provides a mechanism for specificity of E2F function. EMBO J 2002, 21:5775-5786
52. Prives C, Hall PA: The p53 pathway. J Pathol 1999, 187:112-126
53. Ren B, Cam H, Takahashi Y, Volkert T, Terragni J, Young RA, Dynlacht BD: E2F integrates cell cycle progression with DNA repair, replication, and G(2)/M checkpoints. Genes Dev 2002, 16:245-256
54. Shreeram S, Sparks A, Lane DP, Blow JJ: Cell type-specific responses of human cells to inhibition of replication licensing. Oncogene 2002, 21:6624-6632
55. Wohlschlegel JA, Kutok JL, Weng AP, Dutta A: Expression of geminin as a marker of cell proliferation in normal tissues and malignancies. Am J Pathol 2002, 161:267-273