Colorimetric enzymatic assay of L-malic acid using dehydrogenase from baker's yeast

Food Technology and Biotechnology

Volumn 46, Issue 2, 2008, Pages 229-233

  Peres, Maristela F S ^a   Laluce, Cecilia ^a   Gattás, Edwil A L ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

Baker's yeast; Colorimetric assay of L malic acid; Fruit juices; Malate dehydrogenase (MDH); Wine

Indexed keywords

BAKER'S YEAST; COLORIMETRIC METHODS; ENZYMATIC ASSAY; ENZYMATIC PREPARATION; L-MALIC ACID; LOWER DETECTION LIMIT; MALATE DEHYDROGENASE; STANDARD DEVIATION;

COLOR; FRUIT JUICES; OPTIMIZATION; WINE;

COLORIMETRY;

SACCHAROMYCES CEREVISIAE;

EID: 46349111918     PISSN: 13309862     EISSN: 13342606     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (31)

1. C. Mongay, A. Pastor, C. Olmos, Determination of carbo-xylic acids and inorganic anions in wines by ion-exchange chromatography, J. Chromatogr. A, 736 (1996) 351-357.
2. O.E.S. Godinho, N.E. De Souza, L.M. Aleixo, A.V. Ivaska, Determination of tartaric acid and the sum of malic and citric acids in grape juices by potentiometric titration, J. AOAC Int. 71 (1988) 1028-1032.
3. J.L.F.C. Lima, T.I.M.S. Lopes, A.O.S.S. Rangel, Enzymatic determination of 1(+) lactic and 1(-) malic acids in wines by flow-injection spectrophotometry, Anal. Chim. Acta, 366 (1998) 187-191.
4. L. Saavedra, A. García, C. Barbas, Development and validation of a capillary electrophoresis method for direct measurement of isocitric, citric, tartaric and malic acids as adulteration markers in orange juice, J. Chromatogr. A, 881 (2000) 395-401.
5. +-dependent isocitrate dehydrogenase gene (IDH1, IDH2) disruption of sake yeast on organic acid composition in sake mash, J. Biosci Bioeng. 88 (1999) 258-263.
6. H.D. Belitz, W. Grosch: Sugars, Sugar Alcohols and Honey. In: Food Chemistry, Springer-Verlag, Heidelberg, Germany (1987) pp. 592-638.
7. M.A. Segundo, A.O.S.S. Rangel, Kinetic determination of 1(-)malic acid in wines using sequential injection analysis, Anal Chim. Acta, 499 (2003) 99-106.
8. N. Kiba, J. Inagaki, M. Furusawa, Chemiluminometric flow-injection method for determination of free L-malate in wine with co-immobilized malate dehydrogenase/NADH oxidase, Talanta, 42 (1995) 1751-1755.
9. M.L. Morales, A.G. Gonzales, A.M. Troncoso, Ion-exclusion chromatographic determination of organic acids in vinegars, J. Chromatogr. A, 822 (1998) 45-51.
10. V. Galli, C. Barbas, Capillary electrophoresis for the analysis of short-chain organic acids in coffee, J. Chromatogr. A, 1032 (2004) 299-304.
11. E. Finotti, A. Bertone, V. Vivanti, Balance between nutrients and anti-nutrients in nine Italian potato cultivars, Food Chem. 99 (2006) 698-701.
12. K. Haila, J. Ktimpulainen, U. Häkkinen, R. Tahvonen, Sugar and organic acid contents of vegetables consumed in Finland during 1988-1989, J. Food Comp. Anal. 5 (1992) 100-107.
13. S. Roth, Cat8 and Sip4 mediate regulated transcriptional action of the yeast malate dehydrogenase gene MDH2 by three carbon source-responsive promoter elements, Yeast, 18 (2001) 151-162.
14. Y. Peleg, J.S. Rokem, I. Goldberg, A simple plate-assay for the screening of L-malic acid producing microorganisms, FEMS Microbiol. Lett. 67 (1990) 233-236.
15. L. Rotariu, C. Bala, V. Magearu, New potentiometric mi-crobial biosensor for ethanol determination in alcoholic beverages, Anal. Chim. Acta, 513 (2004) 119-123.
16. H. Léric, J.C. Kaplan, G. Broun, A colorimetric micromethod for the enzymatic assay of ethanol in blood, Clin. Chim. Acta, 29 (1970) 523-528.
17. H.H. Lim, J.E. Buttery, Determination of ethanol in serum by an enzymatic pms-int colorimetric method, Clin. Chim. Acta, 75 (1977) 9-12.
18. F.P. Altman, Tetrazolium salts and formazans, Progr. Histochem. Cytochem. 9 (1976) 1-56.
19. T. Mosmann, Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays, J. Immunol. Methods, 65 (1983) 55-63.
20. D. Schiff, G. Chan, M.J. Poznansky, Bilirubin toxicity in neural cell lines N115 and NBR10A, Pediatr. Res. 19 (1985) 908-911.
21. Z. Datki, A. Juhász, M. Gálfi, K. Soós, R. Papp, D. Zádori, B. Penke, Method for measuring neurotoxicity of aggregating polypeptides with the MTT assay on differentiated neuroblastoma cells, Brain Res. Bull. 62 (2003) 223-229.
22. Y. Liu, D.A. Peterson, H. Kimura, D. Schubert, Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction, J. Neurochem. 69 (1997) 581-593.
23. E.A.L. Gattás, M.F.S. Peres, Production of glycerol-3-phos-phate dehydrogenase from baker's yeast, Braz. J. Food Nutr. 14 (2003) 205-209 (in Portuguese).
24. N. Matsuki, K. Abe, Measurement of cellular 3-(4,5-dime-thylthiazol-2-yl) -2,5-diphenyltetrazoliurn bromide (MTT) reduction activity and lactate dehydrogenase release using MTT, Neurosci. Res. 38 (2000) 325-329.
25. G.F. Sprague, J.E. Cronan, Isolation and characterization of Saccharomyces cerevisiae mutants defective in glycerol cata-bolism, J. Bacteriol. 129 (1977) 1335-1342.
26. E. Layne: Spectrophotometric and Turbidimetric Methods. In: Methods in Enzymology, Vol. 3, S.P. Colowick, N.O. Kaplan (Eds.), Elsevier Academic Press, New York, USA (1957) pp. 447-450.
27. W. Atzpodien, J.M. Gancedo, W. Duntze, H. Holzer, Iso-enzymes of malate dehydrogenase in Saccharomyces cerevisiae, Eur. J. Biochem. 7 (1968) 58-62.
28. A.F. Smith: Malate to Oxaloacetate Reaction. In: Methods of Enzymatic Analysis, Vol. 3, H.U. Bergmeyer (Ed.), VCH Ver-lagsgesellschaft, Weinheim, Germany (1983) pp. 166-171.
29. N. Kiba, M. Oguchi, M. Furusawa, Determination of L-malate in wine by flow-injection with co-immobilized malate dehydrogenase/oxaloacetate decarboxylase, Talanta, 40 (1993) 1163-1165.
30. C. Luo, X. Wang, J. Long, J. Liu, An NADH-tetrazolium-coupled sensitive assay for malate dehydrogenase in mitochondria and crude tissue homogenates, J. Biochem. Biophys. Methods, 68 (2006) 101-111.
31. E.A.L. Gattás, C.R.S. Tininis, C. Laluce, Enzymatic assays using preparations containing dehydrogenases obtained from commercial active dry yeast. Brazilian national patent PI0205552-0 (2002).