메뉴 건너뛰기




Volumn 69, Issue 2, 1997, Pages 581-593

Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) reduction

Author keywords

3 (4,5 Dimethylthiazol 2 yl) 2,5 diphenyltetrazolium bromide; Endocytosis; Exocytosis; Oxidoreductase; Viability assay

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; OXIDOREDUCTASE;

EID: 0030852948     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1997.69020581.x     Document Type: Article
Times cited : (889)

References (38)
  • 1
    • 0021318441 scopus 로고
    • Catalase in vitro
    • Aebi H. (1984) Catalase in vitro. Methods Enzymol. 105, 121-126.
    • (1984) Methods Enzymol. , vol.105 , pp. 121-126
    • Aebi, H.1
  • 2
    • 0017247658 scopus 로고
    • Tetrazolium salts and formazans
    • Altman F. P. (1976) Tetrazolium salts and formazans. Prog. Histochem. Cytochem. 9, 1-56.
    • (1976) Prog. Histochem. Cytochem. , vol.9 , pp. 1-56
    • Altman, F.P.1
  • 5
    • 0028233494 scopus 로고
    • Hydrogen peroxide mediates amyloid β protein toxicity
    • Behl C., Davis J. B., Lesley R., and Schubert D. (1994) Hydrogen peroxide mediates amyloid β protein toxicity. Cell 77, 817-827.
    • (1994) Cell , vol.77 , pp. 817-827
    • Behl, C.1    Davis, J.B.2    Lesley, R.3    Schubert, D.4
  • 6
    • 0027270211 scopus 로고
    • Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT): Subcellular localization, substrate dependence and involvement of mitochondrial electron transport in MTT reduction
    • Berridge M. V. and Tan A. N. (1993) Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular localization, substrate dependence and involvement of mitochondrial electron transport in MTT reduction. Arch. Biochem. Biophys. 303, 474-482.
    • (1993) Arch. Biochem. Biophys. , vol.303 , pp. 474-482
    • Berridge, M.V.1    Tan, A.N.2
  • 7
    • 0017185162 scopus 로고
    • The removal of leukocytes and platelets from whole blood
    • Beutler E. and West C. (1976) The removal of leukocytes and platelets from whole blood. J. Clin. Lab. Res. 88, 328-333.
    • (1976) J. Clin. Lab. Res. , vol.88 , pp. 328-333
    • Beutler, E.1    West, C.2
  • 8
    • 0011913143 scopus 로고
    • Bafilomycins: A class of inhibitors of membrane ATPases from microorganism, animal cells, and plant cells
    • Bowman E. J., Siebers A., and Altendorf K. (1988) Bafilomycins: a class of inhibitors of membrane ATPases from microorganism, animal cells, and plant cells. Proc. Natl. Acad. Sci. USA 85, 7972-7976.
    • (1988) Proc. Natl. Acad. Sci. USA , vol.85 , pp. 7972-7976
    • Bowman, E.J.1    Siebers, A.2    Altendorf, K.3
  • 9
    • 0027351844 scopus 로고
    • Reduction of a tetrazolium salt and superoxide generation in human tumor cells (HeLa)
    • Burdon R. H., Gill V., and Rice-Evens C. (1993) Reduction of a tetrazolium salt and superoxide generation in human tumor cells (HeLa). Free Radic. Res. Commun. 18, 369-380.
    • (1993) Free Radic. Res. Commun. , vol.18 , pp. 369-380
    • Burdon, R.H.1    Gill, V.2    Rice-Evens, C.3
  • 11
    • 0029134736 scopus 로고
    • Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin
    • Damke H., Baba T., van der Bliek A. M., and Schmid S. L. (1995) Clathrin-independent pinocytosis is induced in cells overexpressing a temperature-sensitive mutant of dynamin. J. Cell Biol. 131, 69-80.
    • (1995) J. Cell Biol. , vol.131 , pp. 69-80
    • Damke, H.1    Baba, T.2    Van Der Bliek, A.M.3    Schmid, S.L.4
  • 12
    • 0025363276 scopus 로고
    • Studies on the mechanisms of autophagy
    • Dunn W. A. Jr. (1990) Studies on the mechanisms of autophagy. J. Cell Biol. 110, 1923-1945.
    • (1990) J. Cell Biol. , vol.110 , pp. 1923-1945
    • Dunn Jr., W.A.1
  • 13
    • 0026559695 scopus 로고
    • Prelysosomal and lysosomal connections between autophagy and endocytosis
    • Gordon P. B., Høyvik H., and Seglen P.O. (1992) Prelysosomal and lysosomal connections between autophagy and endocytosis. Biochem. J. 283, 361-369.
    • (1992) Biochem. J. , vol.283 , pp. 361-369
    • Gordon, P.B.1    Høyvik, H.2    Seglen, P.O.3
  • 14
    • 0024426375 scopus 로고
    • The MTT assay underestimates the growth inhibitory effects of interferon
    • Jabber S. A. B., Twentyman P. R., and Watson J. V. (1989) The MTT assay underestimates the growth inhibitory effects of interferon. Br. J. Cancer 60, 523-528.
    • (1989) Br. J. Cancer , vol.60 , pp. 523-528
    • Jabber, S.A.B.1    Twentyman, P.R.2    Watson, J.V.3
  • 15
    • 0007574262 scopus 로고
    • Localization of mitochondria in living cells with rhodamine 123
    • Johnson L. V., Walsh M. L., and Chen L. B. (1980) Localization of mitochondria in living cells with rhodamine 123. Proc. Natl. Acad. Sci. USA 77, 990-994.
    • (1980) Proc. Natl. Acad. Sci. USA , vol.77 , pp. 990-994
    • Johnson, L.V.1    Walsh, M.L.2    Chen, L.B.3
  • 16
    • 0024448458 scopus 로고
    • Human cells lacking mtDNA: Repopulation with exogenous mitochondria by complementation
    • King M. P. and Attardi G. (1989) Human cells lacking mtDNA: repopulation with exogenous mitochondria by complementation. Science 246, 500-503.
    • (1989) Science , vol.246 , pp. 500-503
    • King, M.P.1    Attardi, G.2
  • 17
    • 0025051251 scopus 로고
    • Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes
    • Kopitz J., Kisen G. Ø., Gorden P. B., Bohley P., and Seglen P. O. (1990) Nonselective autophagy of cytosolic enzymes by isolated rat hepatocytes. J. Cell Biol. 111, 941-953.
    • (1990) J. Cell Biol. , vol.111 , pp. 941-953
    • Kopitz, J.1    Kisen, G.Ø.2    Gorden, P.B.3    Bohley, P.4    Seglen, P.O.5
  • 19
    • 0022555867 scopus 로고
    • Acidification of the endocytic and exocytic pathways
    • Mellman I., Fuchs R., and Helenius A. (1986) Acidification of the endocytic and exocytic pathways. Annu. Rev. Biochem. 55, 663-700.
    • (1986) Annu. Rev. Biochem. , vol.55 , pp. 663-700
    • Mellman, I.1    Fuchs, R.2    Helenius, A.3
  • 20
    • 0021152538 scopus 로고
    • Isolation of mitochondria from ascites tumor cells permeabilized with digitonin
    • Moreadith R. W. and Fiskum G. (1984) Isolation of mitochondria from ascites tumor cells permeabilized with digitonin. Anal. Biochem. 137, 360-367.
    • (1984) Anal. Biochem. , vol.137 , pp. 360-367
    • Moreadith, R.W.1    Fiskum, G.2
  • 21
    • 0021061819 scopus 로고
    • Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity
    • Mosmann T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity. J. Immunol. Methods 65, 55-63.
    • (1983) J. Immunol. Methods , vol.65 , pp. 55-63
    • Mosmann, T.1
  • 22
    • 0028787189 scopus 로고
    • Bcl-2 protects neural cells from cyanide/ aglycemia-induced lipid oxidation, mitochondrial injury, and loss of viability
    • Myers M. K., Fiskum G., Liu Y., Simmens S. J., Bredesen D. E., and Murphy A. N. (1995) Bcl-2 protects neural cells from cyanide/ aglycemia-induced lipid oxidation, mitochondrial injury, and loss of viability. J. Neurochem. 65, 2432-2440.
    • (1995) J. Neurochem. , vol.65 , pp. 2432-2440
    • Myers, M.K.1    Fiskum, G.2    Liu, Y.3    Simmens, S.J.4    Bredesen, D.E.5    Murphy, A.N.6
  • 25
    • 0025744372 scopus 로고
    • A novel fluorescent ceramide analogue for studying membrane traffic in animal cells: Accumulation at the Golgi apparatus results in altered spectral properties of the sphingolipid precursor
    • Pagano R. E., Martin O. C., Kang H. C., and Haugland R. P. (1991) A novel fluorescent ceramide analogue for studying membrane traffic in animal cells: accumulation at the Golgi apparatus results in altered spectral properties of the sphingolipid precursor. J. Cell Biol. 113, 1267-1279.
    • (1991) J. Cell Biol. , vol.113 , pp. 1267-1279
    • Pagano, R.E.1    Martin, O.C.2    Kang, H.C.3    Haugland, R.P.4
  • 26
    • 0029873038 scopus 로고    scopus 로고
    • Fibroblast growth factor-2 protects entorhinal layer II glutamatergic neurons from axotomy-induced cell death
    • Peterson D. A., Lucidi-Phillipi C. A., Murphy D. P., Ray J., and Gage F. H. (1996) Fibroblast growth factor-2 protects entorhinal layer II glutamatergic neurons from axotomy-induced cell death. J. Neurosci. 16, 886-898.
    • (1996) J. Neurosci. , vol.16 , pp. 886-898
    • Peterson, D.A.1    Lucidi-Phillipi, C.A.2    Murphy, D.P.3    Ray, J.4    Gage, F.H.5
  • 27
    • 0026627966 scopus 로고
    • Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: Effects of brefeldin A and G protein activators
    • Robinson M. S. and Kreis T. E. (1992) Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: effects of brefeldin A and G protein activators. Cell 69, 129-138.
    • (1992) Cell , vol.69 , pp. 129-138
    • Robinson, M.S.1    Kreis, T.E.2
  • 28
    • 0030053909 scopus 로고    scopus 로고
    • Membrane dynamics in endocytosis
    • Robinson M. S., Watts C., and Zerial M. (1996) Membrane dynamics in endocytosis. Cell 84, 13-21.
    • (1996) Cell , vol.84 , pp. 13-21
    • Robinson, M.S.1    Watts, C.2    Zerial, M.3
  • 29
    • 0023597602 scopus 로고
    • Cerebral ischemia and reperfusion: Prevention of brain mitochondrial injury by lidoflazize
    • Rosenthal R. E., Hamud F., Fiskum G., Varghese P. J., and Sharpe S. (1987) Cerebral ischemia and reperfusion: prevention of brain mitochondrial injury by lidoflazize. J. Cereb. Blood Flow Metab. 7, 752-758.
    • (1987) J. Cereb. Blood Flow Metab. , vol.7 , pp. 752-758
    • Rosenthal, R.E.1    Hamud, F.2    Fiskum, G.3    Varghese, P.J.4    Sharpe, S.5
  • 31
    • 0029040824 scopus 로고
    • The intracellular component of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction is specifically inhibited by β-amyloid peptides
    • Shearman M. S., Hawtin S. R., and Tailor V. J. (1995) The intracellular component of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction is specifically inhibited by β-amyloid peptides. J. Neurochem. 65, 218-227.
    • (1995) J. Neurochem. , vol.65 , pp. 218-227
    • Shearman, M.S.1    Hawtin, S.R.2    Tailor, V.J.3
  • 32
    • 50549158051 scopus 로고
    • Studies on succinatetetrazolium reductase system. III. Points of coupling of four different tetrazolium salts
    • Slater T. F., Sawyer B., and Sträuli U. (1963) Studies on succinatetetrazolium reductase system. III. Points of coupling of four different tetrazolium salts. Biochim. Biophys. Acta 77, 383-393.
    • (1963) Biochim. Biophys. Acta , vol.77 , pp. 383-393
    • Slater, T.F.1    Sawyer, B.2    Sträuli, U.3
  • 34
    • 0021923854 scopus 로고
    • Phorbol esters and horseradish peroxidase stimulate pinocytosis and redirect the flow of pinocytosed fluid in macrophages
    • Swanson J. A., Yirinec B. D., and Silverstein S. C. (1985) Phorbol esters and horseradish peroxidase stimulate pinocytosis and redirect the flow of pinocytosed fluid in macrophages. J. Cell Biol. 100, 851-859.
    • (1985) J. Cell Biol. , vol.100 , pp. 851-859
    • Swanson, J.A.1    Yirinec, B.D.2    Silverstein, S.C.3
  • 35
    • 0006847485 scopus 로고
    • Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation
    • Szoka F. and Papahadjopoulos D. (1978) Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation. Proc. Natl. Acad. Sci. USA 75, 4194-4198.
    • (1978) Proc. Natl. Acad. Sci. USA , vol.75 , pp. 4194-4198
    • Szoka, F.1    Papahadjopoulos, D.2
  • 36
    • 0021748350 scopus 로고
    • Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes
    • Terasaki M., Song J., Wong J. R., Weiss M. J., and Chen L. B. (1984) Localization of endoplasmic reticulum in living and glutaraldehyde-fixed cells with fluorescent dyes. Cell 38, 101-108.
    • (1984) Cell , vol.38 , pp. 101-108
    • Terasaki, M.1    Song, J.2    Wong, J.R.3    Weiss, M.J.4    Chen, L.B.5
  • 37
    • 0025738516 scopus 로고
    • Tetrazolium-based assays for cellular viability: A critical examination of selected parameters affecting formazan production
    • Vistica D. T., Skehan P., Scudiero D., Monks A., and Boyd M. R. (1991) Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production. Cancer Res. 51, 2515-2520.
    • (1991) Cancer Res. , vol.51 , pp. 2515-2520
    • Vistica, D.T.1    Skehan, P.2    Scudiero, D.3    Monks, A.4    Boyd, M.R.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.